Synchronization of plant cells in culture and in meristems by aphidicolin

Author(s):  
Francesco Sala ◽  
Maria Grazia Galli ◽  
Guido Pedrali-Noy ◽  
Silvio Spadari
Keyword(s):  
2007 ◽  
Vol 7 (3) ◽  
pp. 599-605 ◽  
Author(s):  
Cornelia Schürch ◽  
Peter Blum ◽  
Fred Zülli
Keyword(s):  

1988 ◽  
Vol 66 (9) ◽  
pp. 1707-1716 ◽  
Author(s):  
Carol Weerdenburg ◽  
Robert W. Seagull

Treatment with 10 μM taxol or 3 mM colchicine results in the development of similar aberrant morphotypes in Vicia hajastana (vetch) suspension cultures. After 2 days exposure to either drug, cells exhibit a disruption in polarity (i.e., swelling or spherical morphology). In taxol-treated cells, microtubules are abundant and brightly stained. In the presence of colchicine, microtubules are absent. Calcofluor staining illustrates that in the presence of taxol the microfibrils appear as thick ordered bundles, while in the presence of colchicine, microfibril bundles are smaller and exhibit less detectable order. The presence of thick bundles of microfibrils is confirmed in taxol-treated cells by the analysis of birefringence patterns. Walls of colchicine-treated cells exhibit a patchy birefringence pattern in polarized light, with adjacent patches exhibiting different order. Although taxol-treated cells contain abundant microtubules, their morphology and wall deposition resemble those of cells that lack microtubules. These observations indicate that the dynamics of microtubules may be important to their proper functioning in cell wall deposition. The differences in wall morphology between taxol- and colchicine-treated cells may reflect the different mechanisms of action of these two drugs.


Plant Science ◽  
1987 ◽  
Vol 51 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Daniela Bellincampi ◽  
Giorgio Morpurgo

Coincident with the more spectacular developments in biology of the last two decades there has quietly emerged a new technology with far-reaching potential, namely the growth and manipulation of animal and plant cells in culture. It has naturally led to an emphasis on homogeneous populations of isolated cells as a step towards simplifying the formidable complexities of even the simplest multicellular tissues. In this way cell culture tends to make microbiologists of us all, followers in the footsteps of Anthony van Leeuwenhoek himself. The microbiological approach is highly productive in the present limited state of knowledge, but will be even more productive if the special properties of metazoan as opposed to protozoan cells can be preserved and analysed. Metazoan cells, in particular, are highly social, and many properties, which may be missed in cultures, are dependent on interactions between the cells. These may be either long range, mediated for example by hormones circulating widely in body fluids, but with high specificity for certain target cells, or short range, at nerve synapses for example, or in growth following wounds.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sinilal Bhaskaran ◽  
Nilesh Sharma ◽  
Pooja Tiwari ◽  
Shree R. Singh ◽  
Shivendra V. Sahi

Author(s):  
Tetsuro Mimura ◽  
Hiroshi Ashihara
Keyword(s):  

Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.


Author(s):  
G. M. Hutchins ◽  
J. S. Gardner

Cytokinins are plant hormones that play a large and incompletely understood role in the life-cycle of plants. The goal of this study was to determine what roles cytokinins play in the morphological development of wheat. To achieve any real success in altering the development and growth of wheat, the cytokinins must be applied directly to the apical meristem, or spike of the plant. It is in this region that the plant cells are actively undergoing mitosis. Kinetin and Zeatin were the two cytokinins chosen for this experiment. Kinetin is an artificial hormone that was originally extracted from old or heated DNA. Kinetin is easily made from the reaction of adenine and furfuryl alcohol. Zeatin is a naturally occurring hormone found in corn, wheat, and many other plants.Chinese Spring Wheat (Triticum aestivum L.) was used for this experiment. Prior to planting, the seeds were germinated in a moist environment for 72 hours.


Author(s):  
Ann Cleary

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.


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