189 Cellular cooperation in the expression of murine delayed-type hypersensitivity (DTH): Augmenting accessory cells bear I-A and appear MHC-restricted

1985 ◽  
Vol 75 (1) ◽  
pp. 152
Author(s):  
H SCOVERN ◽  
C REINPRECHT
Keyword(s):  
Delayed Type Hypersensitivity ◽  
Accessory Cells ◽  
Cellular Cooperation

scholarly journals Site of action of serum factors that block delayed-type hypersensitivity in mice.

1978 ◽  
Vol 148 (1) ◽  
pp. 235-245 ◽  
Author(s):  
P H Lagrange ◽  
G B Mackaness
Keyword(s):  
Antibody Formation ◽  
Intravenous Dose ◽  
Delayed Type Hypersensitivity ◽  
Reaction Site ◽  
Cell Transfer ◽  
Serum Factors ◽  
Accessory Cells ◽  
Site Of Action ◽  
Blocking Factors

When mice have been rendered anergic by a large intravenous dose of sheep erythrocytes, their inability to mount a delayed-type hypersensitivity (DTH) reaction is not due to an absence of mediator cells, for these can be detected in the spleen by cell transfer. Nor is it due to disappearance of accessory cells (monocytes) from circulation. The serum of anergic mice contains blocking factors which are more abundant after absorption with antigen. Such factors are unable to inactivate the mediators of DTH in vitro, nor do they suppress a DTH reaction when introduced locally into the reaction site. They are active, however, when given intravenously to systemically sensitized mice, provided that the sensitized animal has an intact spleen. If the spleen has been removed or the recipients of sensitized cells have been treated with cyclophosphamide before cell transfer, blocking factors are no longer able to suppress a DTH reaction. Reasons are given for the belief that suppression of DTH in animals undergoing a vigorous antibody response is due to the diversion of reactive cells from circulation to undertake an alternative role in antibody formation in the spleen.

scholarly journals Genetic regulation of delayed-type hypersensitivity responses to poly(LTyr,LGlu)-poly(DLAla)--poly(LLys). II. Evidence for a T-T-cell collaboration in delayed-type hypersensitivity responses and for a T-cell defect at the efferent phase in nonresponder H-2k mice.

1980 ◽  
Vol 151 (3) ◽  
pp. 628-636 ◽  
Author(s):  
G Strassmann ◽  
Z Eshhar ◽  
E Mozes
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cellular Response ◽  
Bone Marrow Cells ◽  
Genetic Regulation ◽  
Genetic Defect ◽  
Delayed Type Hypersensitivity ◽  
Accessory Cells ◽  
Cell Defect ◽  
Thymectomized Mice

The intercellular interactions and the site of the genetic defect in delayed-type hypersensitivity (DTH) response to poly(LTyr,LGlu)-poly(DLAla)--poly(LLys) [(T,G)-A--L] has been studied in a system where the T-cell education phase was separated from the efferent phase. In the cellular response, T-T-cell collaboration is required, because T cell-depleted mice were unable to manifest DTH responses after they were transferred with educated and irradiated T cells. Reconstitution of adult thymectomized mice that were irradiated and supplemented with bone marrow cells after treatment with anti-Thy-1.2 serum and complement, with T cells but not with accessory cells gave rise to significant responses. Educated, radioresistant cells required the presence of normal radiosensitive T cells for successful DTH responses to (T,G)-A--L. The genetic defect of nonresponder H-2k and H-2a mice has been located in the above-mentioned, second T-cell population that participates in the efferent phase of this immune reaction. Further characterization revealed that the educated cells are of the Lyt1+ phenotype and that the second normal T cells are expressing the Lyt 1+,2+,3+ phenotype. Thus, the genetic defect of H-2k and H-2a mice in the DTH response to (T,G)-A--L is expressed on the non-antigen-stimulated Lyt 1+,2+,3+ T cells.

scholarly journals Biological functions of t cell lines with specificity for the intracellular bacterium Listeria monocytogenes in vitro and in vivo.

1982 ◽  
Vol 155 (6) ◽  
pp. 1754-1765 ◽  
Author(s):  
S H Kaufmann ◽  
H Hahn
Keyword(s):  
Listeria Monocytogenes ◽  
T Cell ◽  
Cell Lines ◽  
Lower Layer ◽  
Delayed Type Hypersensitivity ◽  
Biological Functions ◽  
Accessory Cells ◽  
T Cell Lines

Peritoneal exudate T lymphocytes from mice immunized with live Listeria monocytogenes were cloned in double-layer soft agar containing heat-killed L. monocytogenes (lower layer) and syngeneic accessory cells (upper layer). Colony-derived T cells were propagated in vitro in the presence of listerial antigen, syngeneic accessory cells, and T cell growth factor. In vitro proliferation, interleukin secretion, and bystander help for B cells of six such T cell lines and several sublines derived from them were found to be antigen dependent and restricted by the H-2IA locus of the major histocompatibility complex. In vivo, these T cell lines conferred delayed-type hypersensitivity to listerial antigen and protection to live L. monocytogenes. It is concluded that different biological functions of acquired antibacterial immunity can be mediated by a single T cell population.

scholarly journals T cells cooperating in the induction of delayed-type hypersensitivity act via the linked recognition of antigenic determinants.

1982 ◽  
Vol 155 (4) ◽  
pp. 1037-1049 ◽  
Author(s):  
M J Tucker ◽  
P A Bretscher
Keyword(s):  
T Cells ◽  
Cell Population ◽  
Gamma Globulin ◽  
Culture Conditions ◽  
Delayed Type Hypersensitivity ◽  
Antigenic Determinants ◽  
Spleen Cells ◽  
Physical Linkage ◽  
A Cell ◽  
Cellular Cooperation

Culture conditions have been established that allow the induction of delayed-type hypersensitivity (DTH) precursor cells present in a cell population derived from unsensitized spleen cells only when antigen-specific, radioresistant, Thy-1-bearing helper cells are added. This specific cellular cooperation acts via the linked recognition of two determinants on the antigen; thus, cells primed to the protein antigen fowl gamma globulin (FGG) will only allow the induction of DTH reactivity against the second antigen, burro erythrocyte (BRBC), when the conjugate FGG-BRBC is present in the cultures. The requirement for physical linkage between the two antigens has been demonstrated by the observation that DTH to BRBC is induced when the conjugate FGG-BRBC is present and not when BRBC and FGG are given an uncoupled molecules.

UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

2019 ◽  
Vol 1 (2) ◽  
pp. 11-16
Author(s):  
Yanna Rotua Sihombing ◽  
Debi Dinha Sitepu
Keyword(s):  
Treatment Group ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno®  32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno®  32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

Performance of Different Solid and Liquid Culture Media for the Improvement of Tuberculin Production

2020 ◽  
Keyword(s):  
Culture Media ◽  
Synthetic Medium ◽  
Chemical Properties ◽  
Economic Loss ◽  
Delayed Type Hypersensitivity ◽  
Basic Medium ◽  
Primary Methods ◽  
Lowenstein Jensen ◽  
Time Required ◽  
Modified Media

Tuberculosis (TB) is one of the most important zoonotic bacterial diseases. A huge economic loss which could be direct or indirect are associated with the disease. Currently, the primary methods used for detection of TB in humans and cattle include the measurement of a delayed type hypersensitivity to purified protein derivative (PPD). So, the need for preparation of purified PPD with adequate properties and increasing the final PPD yield with decreasing the time of tuberculin production has stimulated the interest in the development of its preparation. Our study was performed to compare between the standard and modified media for improving tuberculin production. Middle brook 7H10 agar medium was used as a modified basic medium for mycobacterial growth, followed by cultivation of mycobacteria on Middle brook 7H9 broth medium. For the production, strains were inoculated onto the culture medium (Dorest Henly synthetic medium). Other steps for tuberculin production was done according to standard Weighbridge protocol. The results demonstrated that the using of both Middle brook 7H10 agar and Middle brook 7H9 broth instead of Lowenstein-Jensen (LJ) and glycerin broth media which used in currently produced tuberculin, have better physical and chemical properties. In addition, reducing the time required for production by accelerating the time of microbial growth. Also, it was found that the tuberculin produced using modified media was slightly more potent or the same as currently tuberculin produced. So, both Middle brook 7H10 agar and Middle brook 7H9 broth media are recommended for production of tuberculin saving time and increasing potency of the product but more investigation was recommended for estimation types of protein present in both locally prepared and modified tuberculin.

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