AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH PEPAYA CALIFORNIA (Carica papaya L) TERHADAP BAKTERI Escherchia coli

Infectious diseases caused by bacteria, require antibiotics for treatment. Incorrect use of antibiotics causes. the occurrence of resistance. From the research, it was found that many medicinal plants can be used to cure infectious diseases. One of them is California Papaya Fruit Skin (Carica papaya L). The purpose of this study was to determine the active substance in the skin of the Carica Papaya Fruit as an antibacterial agent and to determine the activity of KBPC extract against E. coli bacteria. This research method is a laboratory experiment where the sample in this study is 3 kg. Phytochemical test results with positive ethanol extraction containing flavonoids, saponins, steroids. Where favonoids can be potential as antibacterial through 3 mechanisms, namely by inhibiting nucleic acid synthesis, inhibiting cell membrane function and inhibiting energy metabolism. The results of the antibacterial activity test against E. coli bacteria were carried out with several concentrations, namely 20%, 40%, 60%, 80%, 100%, the test results obtained from the concentration of 20% of 8.76 mm the highest inhibitory power at the concentration 100% of 19.33 mm. Conclusion KBPC extract has antibacterial activity against E. Coli bacteria.

EFEKTIVITAS SALEP KOMBINASI EKSTRAK DAUN BANGUN – BANGUN (Coleus amboinicus Lour.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) SEBAGAI OBAT LUKA SAYAT

Bangun-bangun leaves and palm leaves contain various secondary metabolites such as tannins, alkaloids and flavonoids which can healing wound. The aim of this study was to determine the effect of ointment combination of ethanol extract of leaves of bangun- bangun (Coleus amboinicus Lour.) And ethanol extract of palm leaves (Elaeis guineensis Jacq.) In healing wound. The bangun- bangun leaves and the palm leaves are separated from the petiole and then dried in a drying cupboard so that it becomes a simplisia. Simplisia is extracted by maceration method then evaporated with a rotary evaporator and evaporated again on a water bath to produce a thick extract. Thick extracts from the leaves of bangun- bangun and palm leaves are then formulated into ointment preparations with varying concentrations. Each rabbit was shaved on its back then cleaned with 70% alcohol. Furthermore, rabbits were anesthetized using 0.5 ml Lidocain HCL as much as 0.5 ml subcutaneously. Next mark the part that will be injured with a diameter of 2 cm, by lifting the rabbit skin using tweezers and then made a wound using surgical scissors that have been sterilized first with 70% alcohol. Wound diameter measurements showed that all treatment groups from day 1 to day 23 experienced changes in wound diameter. The combination ointment of ethanol extract of leaves wake-up (EEDB) 10% and ethanol extract of palm oil leaves (EEDKS) 10% have a more effective effect in wound healing than single dose.

PERBANDINGAN EFEK PENYEMBUHAN LUKA BAKAR ANTARA GEL EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.)DENGAN GEL EKSTRAK ETANOL DAUN KELAPA SAWIT ( Elaeis guineensis Jacq.) SERTA KOMBINASINYA PADA KELINCI TAHUN 2019

African leaves (Gymnanthemum amygdalinum Del.) And palm leaves (Elaeis guineensis Jacq.) Have secondary metabolites, namely alkaloids, steroids / terpenoids, flavonoids, tannins, and saponins in this study aimed to determine the effectiveness of healing burns. The extract was made by maceration method using 96% ethanol. This test used 3 rabbits, each of which had been burnt into 6 wound areas on the back of the rabbit. First group (positive control), second group (without treatment), third group gel with Arfika leaf ethanol extract concentration of 5%, fourth group gel with palm ethanol extract concentration of 7.5%, fifth group of groups gel with a combination of Arfika leaf ethanol extract concentration of 5% and 7.5% palm oil leaf ethanol extract The burns that have been made are smeared with as much test preparation as possible once a day. The next day the diameter of the wound was measured, then smeared with an adequate amount of gel. This is done until the diameter of the wound is equal to zero or the wound has been covered by new tissue. The results of testing the effectiveness of healing burns showed that all treatment groups had a faster recovery day compared to negative controls. The conclusion of this study is the combination of 5% arfika ethanol extract gel and 7.5% oil palm leaf ethanol extract is the group with the fastest fastest day on the 19th day.

Uji Efektivitas GELL Obat Jerawat Dari Ekstrak Etanol Buah Pare (Momordica charantia L.) Terhadap Pertumbuhan Bakteri Propionibacterium acnes

Bacterial infection is a major factor in causing acne. The causes of acne are many (multifactorial), including genetic, endocrine, dietary factors, the activity of the sebaceous glands themselves, psychological factors, mucin, infection by Propionibacterium acne, cosmetics, and other chemicals. Pare extract can be formulated as a gell pimple, which can inhibit the growth of propionibacterium acne. The samples in this study were gell preparations of the ethanol extract of bitter melon with a concentration of 5%, 7.5%, 10%. Based on the results obtained, the addition of the ethanol extract concentration of bitter melon fruit depends on the extract produced. The higher the concentration of gell preparations, the better it is to inhibit bacterial growth. The results showing that pare fruit extract with a concetration of 5%, 7,5%, 10%, and as gell verile 0,025% get a variety of results. And a good resistance zone on the three concentrations that are most close to verile effectiveness is 10% concentration. In conclusion, bitter melon extract can be formulated as an acne medication that can inhibit the growth of the Propionibacterium acnes bacteria.

PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

PENETAPAN KADAR KAFEIN PADA BUBUK TEH HITAM YANG BEREDAR DI PASAR DELI TUA MENGGUNAKAN SPEKTROFOTOMETRI UV

Tea is a drink that is very familiar in the world and is very common in everyday life. Tea is also the most consumed and favored beverage by the public after water. Besides being beneficial, tea also contains compounds that have a negative impact on the body, namely caffeine compounds. Caffeine is an alkaloid of the methylxanthine group which plays a role in increasing the work of psychomotor in the body, and side effects that can be caused are anxiety, irregular heartbeat, difficulty sleeping, tremors, diuresis and others. The purpose of this study was to determine the caffeine content in black tea powder circulating in the Old Deli market. This type of research is descriptive with a purposive sampling method. The analytical method used is qualitative with murexide and quantitative ultraviolet spectrophotometry with a wavelength of 267nm. The results of a qualitative analysis of all positive samples contained caffeine. The average quantitative yield of caffeine in brand A black tea powder was (4,82 ±0,0356)%, B brand black tea powder (4,89 ± 0,0173)% and C brand black tea powder (4,93 ± 0,0531)%. The validation test results obtained linearity r = 0,9984, LOD and LOQ is 0,8074 mcg/ml and 2,6914 mcg/ml ,% recovery = 100,07%, RSD = 0,33%. Based on the results of research conducted, it can be concluded that ultraviolet spectrophotometry can be used to determine caffeine levels in black tea powder.

EFEKTIVITAS EKSTRAK ETANOL DAUN EFEKTIVITAS EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS JANTAN GALUR WISTAR HIPERURISEMIA

Sidaguri leaves (Sida rhombifolia L.) Have been shown to contain flavonoid compounds, where they are efficacious as hyperuricemia. This study aims to determine effectiveness ethanol extract of sidaguri leaves as antihyperuricemia in white rats. The study was conducted using the method of potassium oxanate induction and measured using glucometers. 15 animals were divided into 5 groups, namely negative control, positive control, group I combination of ethanol extract of sidaguri leaves 50 mg / kg body weight, group II ethanol extract of sidaguri leaves 100 mg / kg body weight, group III ethanol extract of sidaguri leaves 200 mg / kg body weight for 5 hours. Examination of antihyperuricemia effects is done by comparing the uric acid levels value of the rats before and after treatment. The results of this study indicate that the three dose ethanol extract of sidaguri leaves provide an antihyperuricemia effect and based on one-way Anova, Tuckey and LSD data analysis, shows that the dose of 50 mg / kg body weight is effective as antihyperuricemia and does not have a significant difference (p> 0.05). The conclusion of this study is that the dose is the most effective in decreases uric acid levels in white rats, which is a dose of 200 mg / kg body weight because it mostly decreased uric acid levels.

FORMULASI OBAT KUMUR GAMBIR DAN UJI EFEKTIVITAS TERHADAP BAKTERI Staphylococcus aureus

Oral hygiene and dental health are very important, there are various kinds of patogen bacteria in the oral cavity, including the bacteria that cause thrush, Staphylococcus aureus. Gambir is the result of extraction containing polyphenol compounds. The main components contained in gambir consist of alkaloids, flavonoids, tannins and saponins. The use of gambir as a mouthwash preparation is an alternative to replace mouthwash preparations which generally contain an antiseptic in the form of alcohol which can trigger oral cancer. This research is to formulate gambir as a mouthwash and determine the differences in the effectiveness of antibacterial on the growth of Staphylococcus aureus with variations in the concentration of 5%, 10%, 15%. Antibacterial activity test using NA media with agar diffusion method. Physical requirements tests include organoleptic tests, pH tests and viscosity testsshowed that the preparation of 5%, 10% and 15% gambir mouthwash had a pH outside the optimum bacterial growth range of 5. Viscosity of mouthwash preparations of 1.30-5.10 Cp. The antibacterial activity of Staphylococcus aureus in F1 did not produce inhibitory zones, F2 was 5.46 mm, F3 was 9.60 mm and F4 was 10.76 mm while in f5 the positive control was 12.10 mm. Conclusio of this study is gambier mouthwash concentration of 5%, 10% and 15% can inhibit the growth of staphylococcus aureus bacteria.

UJI EFEKTIVITAS ANTIDEPRESAN EKSTRAK BATANG SERAI (Cymbopogon nardus (L.) Rendle) TERHADAP MENCIT PUTIH JANTAN (Mus musculus)

Depression is a mood disorder that is characterized by profound and sustained sadness and sadness until the loss of life passion. Lemongrass which contains eugenol is thought to have the potential as an antidepressant that works by inhibiting the Mono Amine Oxidase enzyme. This study aims to determine the effectiveness of antidepressant citronella extract (Cymbopogon nardus (L.) Rendle) on male white mice. This study used 5 treatment groups namely CMC Na 0.5%, amitriptilyn, lemongrass extract dose 1.5 mg/kgBB, 15 mg/kgBB and 150 mg/kgBB. Antidepressant testing was determined based on immobility time using the forsed swim test method. Data analysis using statistics was tested for normality and continued with the One Way Anova method with a 95% confidence level. The results showed that the most effective treatment group as an antidepressant was amitriptilyn followed by lemongrass extract dose 150 mg/kgBB. The conclusion of this study is that lemongrass extract dose 150 mg/kgBB has the effectiveness as an antidepressant but is no more effective than the comparison of amitrip

EFEK PENYEMBUHAN LUKA BAKAR GEL KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Vernonia Amygdalina) DAN EKSTRAKETANOLDAUNBANGUN–BANGUN(ColeusamboinicusLour)

african leaves ( Vernonia Amygdalina )and wake-up leaves (Coleus amboinicus Lour)are plants traditional plants that are used as medicine. Leaves african which is contain flavonoid, saponin, tanin and leaves wake up which is contain flavonoid, tanin, saponin and steroid. This study to find aims out as effect healing of burns.Method : Sample in this study was african leaves and wake-up leaves which were taken purposively without comparing sampel from other regions, then extrated by maceration using ethanol 96 %. Result : Testing of rhe healing effects of burns can be divided in five treatment, positive group using biolacenton , negatif group using basis gel and the last group using the extract ethanol gel EEDA and EEDB concentrations of 4%+2,5%,8%+5%,16%+10%. Given burns to the back of rabbits length with of 2cm, and the gel is applied twice a day for 11 day and diameter measured of the wound. Conclusion : The combination of EEDA and EEDA the ethanol extract the concentration of 16% + 10% was the effective control for healing burns. When compared bioplacenton gel as a healing wound in rabbit.