Detection by a solid phase independent enzyme immunoassay of monoclonal anti-idiotypic antibodies against monoclonal antibodies neutralizing Semliki Forest virus and encephalomyocarditis virus

1993 ◽  
Vol 43 (2) ◽  
pp. 137-146 ◽  
Author(s):  
M. Harmsen ◽  
T.A.M. Oosterlaken ◽  
C. Tangerman ◽  
H. Snippe ◽  
C.A. Kraaijeveld
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Semliki Forest Virus ◽  
Solid Phase ◽  
Encephalomyocarditis Virus

Solid-phase enzyme immunoassay of urokinase using monoclonal antibodies

1983 ◽  
Vol 3 (4) ◽  
pp. 381-388 ◽  
Author(s):  
P. Hérion ◽  
D. Portetelle ◽  
J. -D. Franssen ◽  
J. Urbain ◽  
A. Bollen
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Biological Fluids ◽  
Immunosorbant Assay ◽  
Rapid Procedure ◽  
Preliminary Purification ◽  
Enzyme Linked Immunosorbant Assay

Using two monoclonal antibodies directed against urokinase, we have developed a micro enzyme-linked immunosorbant assay (ELISA) to detect and measure urokinase in biological fluids. The system presents the following characteristics: simple and rapid procedure, reproducibility, sensitivity (urokinase levels down to 1 ng/ml) and evaluation of the enzyme in biological fluids such as urine, pleural elfusions, and ascitic fluids without preliminary purification.

scholarly journals A neutralization-inhibition enzyme immunoassay for anti-idiotypic antibodies that block monoclonal antibodies neutralizing Semliki Forest virus

1988 ◽  
Vol 115 (2) ◽  
pp. 255-261 ◽  
Author(s):  
T.A.M. Oosterlaken ◽  
M. Harmsen ◽  
C. Tangerman ◽  
P. Schielen ◽  
C.A. Kraaijeveld ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Semliki Forest Virus

A solid-phase enzyme immunoassay for the detection of tetanus toxin using human and murine monoclonal antibodies

1991 ◽  
Vol 31 (2) ◽  
pp. 135-140 ◽  
Author(s):  
Stephan T. Kiessig ◽  
Christian Hentschel ◽  
Sigbert Jahn ◽  
Michael Mehl ◽  
Roland Starke ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Tetanus Toxin ◽  
Solid Phase ◽  
Murine Monoclonal Antibodies

Detection of a shared idiotope on two encephalomyocarditis virus neutralizing monoclonal antibodies by neutralization inhibition enzyme immunoassay

1993 ◽  
Vol 44 (2-3) ◽  
pp. 319-328 ◽  
Author(s):  
C.A. Kraaijeveld ◽  
T.A.M. Oosterlaken ◽  
F. Vlaspolder ◽  
P.W. van Dijk ◽  
M. Harmsen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Encephalomyocarditis Virus

Potential of monoclonal antibodies to improve therapeutic monitoring of cyclosporine.

1987 ◽  
Vol 33 (1) ◽  
pp. 32-37 ◽  
Author(s):  
V Quesniaux ◽  
R Tees ◽  
M H Schreier ◽  
G Maurer ◽  
M H van Regenmortel
Keyword(s):  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Cyclosporine A ◽  
Solid Phase ◽  
Therapeutic Monitoring ◽  
High Affinity ◽  
Polyclonal Antisera ◽  
Direct Solid

Abstract We show that monitoring of cyclosporine by immunoassay could be improved by using monoclonal antibodies (MAbs) of restricted specificity instead of polyclonal antisera that recognize both unmodified cyclosporine and its metabolites. MAbs with high affinity for cyclosporine have been prepared and characterized. We tested their ability to discriminate between native cyclosporine and its metabolites in indirect solid-phase enzyme immunoassay with a set of cyclosporine metabolites modified at residues 1, 4, 6, and 9 (corresponding to the six known sites of metabolism of cyclosporine). All the metabolites tested were detected by MAb1 at least 15- to 1000-fold less well than unmodified cyclosporine. A second MAb recognized unmodified cyclosporine and most of its metabolites equally well. Both MAbs retained their activity when coupled to alkaline phosphatase and could therefore be used in a direct solid-phase enzyme immunoassay.

A solid-phase enzyme immunoassay for the common and subtypic determinants of hepatitis B surface antigen with monoclonal antibodies

1986 ◽  
Vol 87 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Sadakazu Usuda ◽  
Fumio Tsuda ◽  
Tohru Gotanda ◽  
Katsumi Tachibana ◽  
Motozumi Nomura ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
The Common
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