Rapid immunotyping of Chlamydia trachomatis with monoclonal antibodies in a solid-phase enzyme immunoassay.

Using two monoclonal antibodies directed against urokinase, we have developed a micro enzyme-linked immunosorbant assay (ELISA) to detect and measure urokinase in biological fluids. The system presents the following characteristics: simple and rapid procedure, reproducibility, sensitivity (urokinase levels down to 1 ng/ml) and evaluation of the enzyme in biological fluids such as urine, pleural elfusions, and ascitic fluids without preliminary purification.

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Generation of Monoclonal Antibodies to Prostatic Acid Phosphatase Isoenzyme 2 And Application in Solid-Phase Enzyme Immunoassay

Biotechnology and Applied Biochemistry ◽

10.1111/j.1470-8744.1989.tb00052.x ◽

1989 ◽

Vol 11 (1) ◽

pp. 89-95 ◽

Cited By ~ 3

Author(s):

CY Kuo ◽

KW Chen ◽

J. Fu ◽

KW Lam ◽

CY Lee

Keyword(s):

Monoclonal Antibodies ◽

Acid Phosphatase ◽

Enzyme Immunoassay ◽

Solid Phase ◽

Prostatic Acid Phosphatase

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A solid-phase enzyme immunoassay for the detection of tetanus toxin using human and murine monoclonal antibodies

Journal of Basic Microbiology ◽

10.1002/jobm.3620310212 ◽

1991 ◽

Vol 31 (2) ◽

pp. 135-140 ◽

Cited By ~ 3

Author(s):

Stephan T. Kiessig ◽

Christian Hentschel ◽

Sigbert Jahn ◽

Michael Mehl ◽

Roland Starke ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Enzyme Immunoassay ◽

Tetanus Toxin ◽

Solid Phase ◽

Murine Monoclonal Antibodies

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Potential of monoclonal antibodies to improve therapeutic monitoring of cyclosporine.

Clinical Chemistry ◽

10.1093/clinchem/33.1.32 ◽

1987 ◽

Vol 33 (1) ◽

pp. 32-37 ◽

Cited By ~ 40

Author(s):

V Quesniaux ◽

R Tees ◽

M H Schreier ◽

G Maurer ◽

M H van Regenmortel

Keyword(s):

Alkaline Phosphatase ◽

Monoclonal Antibodies ◽

Enzyme Immunoassay ◽

Cyclosporine A ◽

Solid Phase ◽

Therapeutic Monitoring ◽

High Affinity ◽

Polyclonal Antisera ◽

Direct Solid

Abstract We show that monitoring of cyclosporine by immunoassay could be improved by using monoclonal antibodies (MAbs) of restricted specificity instead of polyclonal antisera that recognize both unmodified cyclosporine and its metabolites. MAbs with high affinity for cyclosporine have been prepared and characterized. We tested their ability to discriminate between native cyclosporine and its metabolites in indirect solid-phase enzyme immunoassay with a set of cyclosporine metabolites modified at residues 1, 4, 6, and 9 (corresponding to the six known sites of metabolism of cyclosporine). All the metabolites tested were detected by MAb1 at least 15- to 1000-fold less well than unmodified cyclosporine. A second MAb recognized unmodified cyclosporine and most of its metabolites equally well. Both MAbs retained their activity when coupled to alkaline phosphatase and could therefore be used in a direct solid-phase enzyme immunoassay.

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Detection by a solid phase independent enzyme immunoassay of monoclonal anti-idiotypic antibodies against monoclonal antibodies neutralizing Semliki Forest virus and encephalomyocarditis virus

Journal of Virological Methods ◽

10.1016/0166-0934(93)90071-x ◽

1993 ◽

Vol 43 (2) ◽

pp. 137-146 ◽

Cited By ~ 1

Author(s):

M. Harmsen ◽

T.A.M. Oosterlaken ◽

C. Tangerman ◽

H. Snippe ◽

C.A. Kraaijeveld

Keyword(s):

Monoclonal Antibodies ◽

Enzyme Immunoassay ◽

Semliki Forest Virus ◽

Solid Phase ◽

Encephalomyocarditis Virus

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A solid-phase enzyme immunoassay for the common and subtypic determinants of hepatitis B surface antigen with monoclonal antibodies

Journal of Immunological Methods ◽

10.1016/0022-1759(86)90532-6 ◽

1986 ◽

Vol 87 (2) ◽

pp. 203-210 ◽

Cited By ~ 33

Author(s):

Sadakazu Usuda ◽

Fumio Tsuda ◽

Tohru Gotanda ◽

Katsumi Tachibana ◽

Motozumi Nomura ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Hepatitis B ◽

Enzyme Immunoassay ◽

Surface Antigen ◽

Solid Phase ◽

Hepatitis B Surface Antigen ◽

The Common

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Development of enzyme immunoassay for detecting I and II types of shiga-like toxins

Токсикологический вестник ◽

10.36946/0869-7922-2021-29-5-43-48 ◽

2021 ◽

Vol 29 (5) ◽

pp. 43-48

Author(s):

Galina Viktorovna Kuklina ◽

Denis Valerievich Pechenkin ◽

Sergei Sergeevich Ipatov ◽

Andrei Valentinovich Eremkin ◽

Aleksei Aleksandrovich Kytmanov ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Enzyme Immunoassay ◽

Solid Phase ◽

Sandwich Elisa ◽

Central Research Institute ◽

Exchange Chromatography ◽

Hybridoma Cells ◽

Federal State ◽

Central Research ◽

Freeze Dried

Introduction. The aim of the work was development of enzyme immunoassay for detecting I and II types of shiga-like toxins and assessment of it diagnostic properties. Materials and methods. For the research, we used hybridomas producing monoclonal antibodies to shiga-like toxins of types I and II, obtained at the branch of the Federal State Budgetary Institution “48 Central Research Institute” of the Ministry of Defense of Russian Federation (Kirov); BALB/c mice; shiga-like toxins of types I and II. Hybridoma cells were cultured in culture flasks and in the peritoneal cavity of BALB/c mice. Monoclonal antibodies were isolated from ascitic fluids by precipitation with a saturated solution of ammonium sulfate, followed by purification by ion exchange chromatography. The obtained preparations of monoclonal antibodies were used to develop enzyme immunoassay for the detection of shiga-like toxins of types I and II. Specific components of enzyme immunoassay were freeze-dried in a protective environment. Results. As a result of research, preparative quantities of monoclonal antibodies against I and II types of shiga-like toxins were obtained and purified; selection of monoclonal antibodies for sorption on the solid phase and for the synthesis of immunoperoxidase conjugates was carried out. Conclusion. experimental enzyme immunoassay allowing to identify 1 ng/ml I and II types of shiga-like toxins in «sandwich»-ELISA was developed.

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