Transferring molecular biology to the clinical laboratory

This document describes the introduction of the concept of property in the field of molecular biology for the presentation of results of clinical laboratory investigations of genes and mutations. It follows the IUPAC–IFCC systematic rules and attempts to create a common base for communication between the clinical laboratory sciences, the medical practitioner, and the molecular biology areas of knowledge.Because of the plethora of possible structural variations in the outcome of analysis, the designation of components is restricted to the symbols of genes as identified by the Human Genome Nomenclature Database (HUGO). The listing of properties having DNA as system comprises all symbols in the HUGO database except for symbols related to mitochondrial genes, while properties having RNA as system are included only when known to be in actual use. The detailed and accurate presentation of results is made by the laboratory performing the study, and it is recommended that the explicit guidelines given by Antonarakis and den Dunnen be adhered to.For electronic communication, a code (NPU) is allocated to each property identified. The complete list of properties, which is an integral part of this report, can be accessed at <www.iupac.org/publications/pac/2004/7609/7609x1799.html>.

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Quantities and units for electrophoresis in the clinical laboratory

Journal of Automatic Chemistry ◽

10.1155/s1463924692000014 ◽

1992 ◽

Vol 14 (1) ◽

pp. 1-4

Author(s):

G. Férard

Keyword(s):

Molecular Biology ◽

Clinical Laboratory ◽

International System ◽

International Organization ◽

International Organization For Standardization ◽

Clinical Laboratories ◽

International System Of Units ◽

System Of Units ◽

Electrophoretic Techniques ◽

Dna Components

Electrophoretic techniques have been developed and refined over decades, and are now widely used in clinical laboratories. For example, electrophoresis is routinely used to separate many different components, including proteins, lipoproteins, and isoenzymes. More recently, the applications of molecular biology in diagnosis have increased the use of electrophoresis to separate DNA components in the clinical laboratory. Various kinds of quantities are used for the description of separation procedures. It is the purpose of this document to provide manufacturers and users of electrophoretic techniques with a list of relevant quantities and units consistent with the International System of Units (SI) and standards of the International Organization for Standardization (ISO).

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Harmonization of interpretative comments in laboratory hematology reporting: the recommendations of Working Group on Diagnostic Hematology of the Italian Society of Clinical Chemistry and Clinical Molecular Biology (WGDH-SIBioC)

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2017-0972 ◽

2018 ◽

Vol 0 (0) ◽

Cited By ~ 2

Author(s):

Sabrina Buoro ◽

Giorgio Da Rin ◽

Alessandra Fanelli ◽

Giuseppe Lippi

Keyword(s):

Molecular Biology ◽

Working Group ◽

Clinical Laboratory ◽

Clinical Chemistry ◽

Clinical Information ◽

Italian Society ◽

World Health ◽

Interpretative Comments ◽

Laboratory Hematology

AbstractThe goal of harmonizing laboratory testing is contributing to improving the quality of patient care and ultimately ameliorating patient outcome. The complete blood and leukocyte differential counts are among the most frequently requested clinical laboratory tests. The morphological assessment of peripheral blood cells (PB) through microscopic examination of properly stained blood smears is still considered a hallmark of laboratory hematology. Nevertheless, a variable inter-observer experience and the different terminology used for characterizing cellular abnormalities both contribute to the current lack of harmonization in blood smear revision. In 2014, the Working Group on Diagnostic Hematology of the Italian Society of Clinical Chemistry and Clinical Molecular Biology (WGDH-SIBioC) conducted a national survey, collecting responses from 78 different Italian laboratories. The results of this survey highlighted a lack of harmonization of interpretative comments in hematology, which prompted the WGDH-SIBioC to develop a project on “Harmonization of interpretative comments in the laboratory hematology report”, aimed at identifying appropriate comments and proposing a standardized reporting system. The comments were then revised and updated according to the 2016 revision of the World Health Organization classification of hematologic malignancies. In summary, the purpose of revaluating comments was aimed at: (a) reducing their overall number, (b) standardizing the language, (c) providing information that could be easily comprehended by clinicians and patients, (d) increasing the quality of the clinical information, and (e) suggesting additional diagnostic tests when necessary.

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Neurobiology of Mental Illness

10.1093/med/9780199934959.001.0001 ◽

2013 ◽

Cited By ~ 21

Keyword(s):

Mental Illness ◽

Molecular Biology ◽

Doctoral Students ◽

Clinical Laboratory ◽

Clinical Treatment ◽

Cutting Edge ◽

Levels Of Analysis ◽

Psychiatric Residents ◽

Cellular Physiology ◽

And Behavior

This resource provides information from numerous levels of analysis including molecular biology and genetics, cellular physiology, neuroanatomy, neuropharmacology, epidemiology, and behavior. In doing so it translates information from the basic laboratory to the clinical laboratory and finally to clinical treatment. The result is an excellent and cutting-edge resource for psychiatric residents, psychiatric researchers and doctoral students in neurochemistry and the neurosciences.

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Properties and Units in the Clinical Laboratory Sciences Part XVIII. Properties and Units in Clinical Molecular Biology

IUPAC Standards Online ◽

10.1515/iupac.76.0667 ◽

2016 ◽

Author(s):

Pedro Soares De Araujo ◽

Bianca Zingales ◽

Pedro Alía-Ramos ◽

Aurora Blanco-Font ◽

Xavier Fuentes-Arderiu ◽

...

Keyword(s):

Molecular Biology ◽

Clinical Laboratory ◽

Clinical Laboratory Sciences

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Effects of Blackboard Resources Utilization on Students’ Performance in Molecular Biology Course

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqaa161.325 ◽

2020 ◽

Vol 154 (Supplement_1) ◽

pp. S149-S149

Author(s):

B P Kandel ◽

M Hackfeld ◽

J Zhang ◽

F Guo

Keyword(s):

Molecular Biology ◽

Undergraduate Students ◽

Clinical Education ◽

Information Technologies ◽

Clinical Laboratory ◽

Biology Education ◽

Disease Status ◽

Disease Diagnosis ◽

Cross Sectional ◽

Content Item

Abstract Introduction/Objective Molecular diagnostics utilizes structural and functional fundamentals of molecular biology and finds its scope in clinical education for disease diagnosis and monitoring, and evaluation of non-disease status. Time and financial constraints make molecular biology education challenging for both teachers and students. Advances in information technologies have helped teachers to provide a rich resource to students which if properly utilized is expected to generate better teaching-learning outcomes. However, the pattern of utilization of digital resources by students and its impact on their performance is still unknown. The aim of this study was to assess students’ behavior to Blackboard resource utilization and its impact on their performance in molecular biology course. Methods We conducted a retrospective cross-sectional study including all the 46 students (9 graduate and 37 undergraduate students; 14 distant and 32 regular students) enrolled for molecular biology course in the year 2017 and assessed the associations between blackboard resource visits and students’ final weighted total grades. Blackboard resource visit variables included “Tegrity” viewing duration, viewing times, and contents viewed (“My Grades”, “Announcement”, “Discussion”, “Contacts”, “Content Item”). Results Among all students, the most and least visited contents were “Content Item” (261 average hits) and “Contacts” (1.28 average hits), respectively with similar patterns of the visit among all student sub-categories. The longest average Tegrity viewing duration (25 hours) and the highest average Tegrity viewing times (56 hits) were noted among undergraduate distance students. The average grade was higher among graduate students than undergraduate students. The overall blackboard resource visit and the individual category visit among graduate and undergraduate students had no association with students’ final weighted scores. Conclusion Our findings have important implications for the utility of blackboard resources among graduate and undergraduate clinical laboratory science students. Similar assessments including students from many different years are recommended to generate more accurate evidence.

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Electron Microscopy in Molecular Biology

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060027 ◽

1967 ◽

Vol 25 ◽

pp. 2-3

Author(s):

Cecil E. Hall

Keyword(s):

Electron Microscopy ◽

Molecular Biology ◽

Noise Level ◽

Molecular Structures ◽

Material Structure ◽

The Arts ◽

Shadow Casting ◽

Electron Image ◽

High Degree ◽

Very High

The visualization of organic macromolecules such as proteins, nucleic acids, viruses and virus components has reached its high degree of effectiveness owing to refinements and reliability of instruments and to the invention of methods for enhancing the structure of these materials within the electron image. The latter techniques have been most important because what can be seen depends upon the molecular and atomic character of the object as modified which is rarely evident in the pristine material. Structure may thus be displayed by the arts of positive and negative staining, shadow casting, replication and other techniques. Enhancement of contrast, which delineates bounds of isolated macromolecules has been effected progressively over the years as illustrated in Figs. 1, 2, 3 and 4 by these methods. We now look to the future wondering what other visions are waiting to be seen. The instrument designers will need to exact from the arts of fabrication the performance that theory has prescribed as well as methods for phase and interference contrast with explorations of the potentialities of very high and very low voltages. Chemistry must play an increasingly important part in future progress by providing specific stain molecules of high visibility, substrates of vanishing “noise” level and means for preservation of molecular structures that usually exist in a solvated condition.

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