A simple kethod of PCR-RFLP pattern analysis for DPA1 allelic typing assignment

1995 ◽  
Vol 44 ◽  
pp. 143
Keyword(s):  
Pattern Analysis ◽  
Rflp Pattern ◽  
Pcr Rflp

scholarly journals Isolation and characterization of escherichia coli in ready-to-eat foods vended in Islamic University, Kushtia

1970 ◽  
Vol 18 ◽  
pp. 99-103 ◽  
Author(s):  
S Biswas ◽  
MAK Parvez ◽  
M Shafiquzzaman ◽  
S Nahar ◽  
MN Rahman
Keyword(s):  
Escherichia Coli ◽  
Pattern Analysis ◽  
University Campus ◽  
Rflp Pattern ◽  
E Coli ◽  
Fish Meat ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Identification And Characterization

Context: Escherichia coli is shed in the feces of warm blooded animals and humans and thus potential for public health. Detection and characterization of E. coli in the ready-to-eat (RTE) foods concerns due to their presence indicates fecal contamination of the food.   Objective: To identify, characterize and RFLP pattern analysis of E. coli isolated from RTE foods vended in Islamic University campus, Kushtia.   Materials and Methods: Fifty samples from four types of consumed foods in six student halls of residence, some temporary restaurants of Islamic University, Kushtia were assessed for bacterial contamination by standard methods. Identification and characterization of E. coli isolates were performed using IMViC tests. Genomic DNA was used to perform RFLP pattern analysis.   Results: Thirty seven out of 50 (74%) examined samples of RTE foods had E. coli contamination. The highest number of E. coli was isolated from vegetable oriented RTE foods (90.90%) and fish, meat and cereals samples were also significantly E. coli positive. RFLP profiling of two E. coli isolates were observed.   Conclusion: The results of this study provide evidence that some RTE foods had unsatisfactory levels of contamination with E. coli. Thus street vended RTE food could be important potential vehicles for food-borne diseases. Molecular characterization may be exploited to identify food borne pathogen among different species.  Keywords: Ready-to-eat foods; Escherichia coli; RFLP pattern DOI: http://dx.doi.org/10.3329/jbs.v18i0.8783 JBS 2010; 18(0): 99-103

scholarly journals Rickettsia helvetica in Ixodes ricinus Ticks in Sweden

1999 ◽  
Vol 37 (2) ◽  
pp. 400-403 ◽  
Author(s):  
Kenneth Nilsson ◽  
Olle Lindquist ◽  
Ai Jie Liu ◽  
Thomas G. T. Jaenson ◽  
Göran Friman ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Citrate Synthase ◽  
Spotted Fever ◽  
Oligonucleotide Primer ◽  
Rrna Gene ◽  
Spotted Fever Group ◽  
Rflp Pattern ◽  
Rickettsia Helvetica ◽  
Pcr Rflp ◽  
Ixodes Ricinus Ticks

In the present study further characterization of the amplified sequence of the citrate synthase gene of the spotted fever groupRickettsia isolated from Ixodes ricinus ticks in Sweden showed that it has 100% homology with the deposited sequence of the citrate synthase gene of Rickettsia helvetica. The restriction fragment length polymorphism (RFLP) pattern of an amplified 382-bp product of the citrate synthase sequence, defined by primers RpCS877 and RpCS1258, yielded fragments for our isolate that could be visualized as a double band that migrated at approximately 44 bp, another double band at 85 bp, and a single band at nearly 120 bp after digestion with the restriction enzyme AluI. When calculating a theoretical PCR-RFLP pattern of the sequence of the citrate synthase gene of R. helvetica from the known positions where the AluI enzyme cuts, we arrived at the same pattern that was obtained for our isolate, a pattern distinctly different from the previously published PCR-RFLP pattern for R. helvetica. Investigation of 125 living I. ricinusticks showed a higher prevalence of rickettsial DNA in these ticks than we had found in an earlier study. Rickettsial DNA was detected by amplification of the 16S rRNA gene, for which a seminested primer system consisting of two oligonucleotide primer pairs was used. Of the 125 ticks, some were pooled, giving a total of 82 tick samples, of which 20 were found to be positive for the rickettsial DNA gene investigated. When considering the fact that some of the positive samples were pooled, the minimum possible prevalence in these ticks was 20 of 125 (16%) and the maximum possible prevalence was 46 of 125 (36.8%). These prevalence estimates conform to those of other studies of spotted fever group rickettsiae in hard ticks in Europe.

Molecular genetic characterization of the cervid strain (‘northern form’) ofEchinococcus granulosus

Parasitology ◽  
1994 ◽  
Vol 109 (2) ◽  
pp. 215-221 ◽  
Author(s):  
J. Bowles ◽  
D. Blair ◽  
D. P. McManus
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Rflp Analysis ◽  
Domestic Sheep ◽  
Rflp Pattern ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Mitochondrial Nd1 ◽  
Molecular Genetic Characterization

SUMMARYWe have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) ofEchinococcus granulosus.PCR–RFLP analysis of the nuclear ITS1 region of the rDNA repeat can readily distinguish the cervid form from other strains ofE. granulosus. The complexity of the RFLP patterns obtained suggests that a number of distinct ITS1 types are present in this strain which may represent an inter-strainE. granulosushybrid. Mitochondrial CO1 sequence of the cervid genotype was ambiguous at 18 positions and closely resembles a cluster of previously characterizedE. granulosusgenotypes, G1 (common, domestic sheep)/G2 (Tasmanian sheep)/G3 (buffalo). In contrast, mitochondrial ND1 sequence, although unique, suggests that the cervid form is most similar to strains represented by the G6 (camel)/G7 (pig) genotypes. We assume that the CO1 and ND1 sequences obtained for the cervid genotype are linked in a single mitochondrial genome although this is difficult to explain if conventional molecular genetics of mitochondrial DNA are assumed. Based on its unique ND1 sequence and ITS1 PCR–RFLP pattern, the cervid strain appears to represent a distinct genotype (designated G8) ofE. granulosus.

scholarly journals Monotypic PCR-RFLP Pattern of Circulating Theileria annulata Isolates from North India Based on HSP 70 gene

2019 ◽  
Vol 15 (1) ◽  
pp. 9-13
Author(s):  
Vikrant Sudan ◽  
Sanjhi Paliwal ◽  
Daya Shanker ◽  
Mukesh Srivastava
Keyword(s):  
Theileria Annulata ◽  
North India ◽  
Rflp Pattern ◽  
Hsp 70 ◽  
Pcr Rflp

scholarly journals Molecular Analysis of H Antigens Reveals that Human DiarrheagenicEscherichia coli O26 Strains That Carry the eaeGene Belong to the H11 Clonal Complex

2000 ◽  
Vol 38 (8) ◽  
pp. 2989-2993 ◽  
Author(s):  
Wen-Lan Zhang ◽  
Martina Bielaszewska ◽  
Jochen Bockemühl ◽  
Herbert Schmidt ◽  
Flemming Scheutz ◽  
...  
Keyword(s):  
Reference Strain ◽  
Fragment Length Polymorphism ◽  
Clonal Complex ◽  
Rflp Analysis ◽  
Rflp Pattern ◽  
E Coli ◽  
Pcr Rflp ◽  
Identical Nucleotide ◽  
Allelic Variations

Fifty-seven Escherichia coli O26 strains isolated from patients in six countries were investigated by PCR restriction fragment length polymorphism (RFLP) analysis of the flagellin-encoding (fliC) gene (fliC RFLP analysis). The strains were determined by serotyping to belong to five different H types or were nonmotile. The fliC RFLP analysis revealed only two different patterns among the 57 strains. One fliC RFLP pattern was displayed by 54 strains and was identical to that ofE. coli H11 reference strain Su4321-41. The otherfliC RFLP pattern was observed for three strains and was identical to that for E. coli H32 reference strain K10. The 54 strains with the H11 fliC RFLP pattern included 22 strains of serotype O26:H11, 23 nonmotile strains, and 9 strains that were initially serotyped as H2, H8, H21, and H32 but that were confirmed to express H11 by repeat serotyping. All 54 strains with the H11 fliC RFLP pattern contained the attaching-and-effacing (eae) gene. The three strains with the H32 fliCRFLP pattern belonged to serotype O26:H32, and all were eaenegative. The fliC genes of 14 selected E. coliO26:H11 strains isolated between 1964 and 1999 had identical nucleotide sequences. Our results demonstrate that E. coli O26 strains that carry the eae gene belong exclusively to the H11 clonal complex. Since there were no H11 fliC allelic variations among the O26 strains tested, E. coli O26:H11 may have emerged recently. The fliC PCR-RFLP test is a reliable, easy-to-perform, and rapid method for determination of the H types of E. coli O26 isolates.

scholarly journals Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Do Ngoc Anh ◽  
Le Tran Anh ◽  
Le Quoc Tuan ◽  
Nguyen Duy Bac ◽  
Tran Viet Tien ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Fasciola Gigantica ◽  
Internal Transcribed Spacers ◽  
Intermediate Form ◽  
Rflp Pattern ◽  
Its1 Sequence ◽  
Central Vietnam ◽  
Helminthic Infection ◽  
Pcr Rflp

Fascioliasis—a disease caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea)—is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam. The aim of this study is to detect the genotype of Fasciola spp. isolated from bovine and buffalo in the Nghe An province, central Vietnam, using PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1). Adult Fasciola spp. were isolated from bile ducts of bovine and buffalo in Nghe An province, Vietnam. Overall, 96 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas. They included 7 samples from infected bovine and 89 samples from infected buffalo. 96/96 samples were identified as Fasciola species by ITS1 of rDNA. In this study, a PCR-RFLP method was used to distinguish between F. hepatica and F. gigantica in ITS1 of rDNA (680 bp) with RsaI restriction enzyme. RFLP pattern with RsaI produced a consistent pattern of 360, 100, and 60 bp fragments in F. hepatica, whereas F. gigantica worms had a profile of 360, 170, and 60 bp in size, respectively. The results showed that using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the ribosomal RNA revealed that 93 out of 96 isolates were of Fasciola gigantica type, whereas three isolates presented an intermediate Fasciola. In the present study, F. gigantica and intermediate form were coexisting in bovine and buffalo in the Nghe An province of central Vietnam, whereas F. hepatica was not detected.

PCR-RFLP pattern of three kinds of Metagonimus in Korea

1997 ◽  
Vol 35 (4) ◽  
pp. 271 ◽  
Author(s):  
J R Yu ◽  
J S Chung ◽  
S Huh ◽  
S H Lee ◽  
J Y Chai
Keyword(s):  
Rflp Pattern ◽  
Pcr Rflp

INTROGRESSIVE HYBRIDIZATION BETWEEN RHODODENDRON KIUSIANUM AND R. KAEMPFERI (ERICACEAE) IN KYUSHU, JAPAN BASED ON CHLOROPLAST DNA MARKERS

2007 ◽  
Vol 64 (3) ◽  
pp. 283-293 ◽  
Author(s):  
N. Kobayashi ◽  
T. Handa ◽  
I. Miyajima ◽  
K. Arisumi ◽  
K. Takayanagi
Keyword(s):  
Chloroplast Dna ◽  
Dna Markers ◽  
Introgressive Hybridization ◽  
Flower Colour ◽  
Natural Hybridization ◽  
Rflp Pattern ◽  
Pcr Rflp ◽  
Chloroplast Dna Markers ◽  
Evergreen Azalea ◽  
Cytoplasmic Introgression

Wild evergreen azalea populations of Rhododendron kiusianum and R. kaempferi (Ericaceae) were analysed using a chloroplast DNA (cpDNA) PCR-RFLP marker that was used to detect introgressive hybridization in our previous study of the Kirishima Mts populations.The populations of the intermediate region in the Unzen Mts, which show phenotypic variation, were demonstrated to result from interspecific hybridization between Rhododendron kiusianum and R. kaempferi, possessing cpDNA from either R. kiusianum (1030/420 bp) or R. kaempferi (950/420/80 bp).Most individuals of Rhododendron kiusianum in the Kujyu Mts, the Aso Mts and the surrounding mountains exhibited the PCR-RFLP pattern of R. kaempferi. These results from the Kujyu Mts and the Aso Mts indicate that natural hybridization and cytoplasmic introgression from Rhododendron kaempferi to R. kiusianum have occurred in the relatively distant past. In the case of Mt Yufudake and Mt Haneyama, the Rhododendron kiusianum population retains the effects of natural hybridization with R. kaempferi in the cpDNA as well as in the variation in flower characteristics.All individuals of Rhododendron kiusianum on Mt Onogaradake in the Takakuma Mts exhibit R. kiusianum cpDNA (1030/420 bp), in spite of variation in flower colour.

Automated Image Analysis Of Nuclear And Cytoplasmic Changes In Exfoliated Cells From Tracheas Treated With Carcinogen

1977 ◽  
Vol 35 ◽  
pp. 220-221
Author(s):  
S.F. Stinson ◽  
J.C. Lilga ◽  
M.B. Sporn
Keyword(s):  
Image Analysis ◽  
Pattern Analysis ◽  
Relative Proportion ◽  
Automated Image Analysis ◽  
Image Analyzer ◽  
Cross Sectional ◽  
Exfoliated Cells ◽  
Neoplastic Lesions ◽  
Analysis System ◽  
Morphologic Criterion

Increased nuclear size, resulting in an increase in the relative proportion of nuclear to cytoplasmic sizes, is an important morphologic criterion for the evaluation of neoplastic and pre-neoplastic cells. This paper describes investigations into the suitability of automated image analysis for quantitating changes in nuclear and cytoplasmic cross-sectional areas in exfoliated cells from tracheas treated with carcinogen.Neoplastic and pre-neoplastic lesions were induced in the tracheas of Syrian hamsters with the carcinogen N-methyl-N-nitrosourea. Cytology samples were collected intra-tracheally with a specially designed catheter (1) and stained by a modified Papanicolaou technique. Three cytology specimens were selected from animals with normal tracheas, 3 from animals with dysplastic changes, and 3 from animals with epidermoid carcinoma. One hundred randomly selected cells on each slide were analyzed with a Bausch and Lomb Pattern Analysis System automated image analyzer.

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