scholarly journals Monotypic PCR-RFLP Pattern of Circulating Theileria annulata Isolates from North India Based on HSP 70 gene

2019 ◽  
Vol 15 (1) ◽  
pp. 9-13
Author(s):  
Vikrant Sudan ◽  
Sanjhi Paliwal ◽  
Daya Shanker ◽  
Mukesh Srivastava
Keyword(s):  
Theileria Annulata ◽  
North India ◽  
Rflp Pattern ◽  
Hsp 70 ◽  
Pcr Rflp

scholarly journals The Trend in Distribution of Q223R Mutation of Leptin Receptor Gene in Amoebic Liver Abscess Patients from North India: A Prospective Study

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Anil Kumar Verma ◽  
Vineet Ahuja ◽  
Jaishree Paul
Keyword(s):  
Liver Abscess ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Rflp Analysis ◽  
North India ◽  
Amoebic Liver Abscess ◽  
Fisher Exact Test ◽  
Exact Test ◽  
Pcr Rflp ◽  
A Prospective Study

Host genetic susceptibility is an important risk factor in infectious diseases. We explored the distribution of Q223R mutation in leptin receptor gene of amoebic liver abscess (ALA) patients of North India. A total of 55 ALA samples along with 102 controls were subjected to PCR-RFLP analysis. The frequency of allele “G” (coding for arginine) was in general high in Indian population irrespective of the disease. Our results of Fisher exact test shows that heterozygous mutant (QQ versus QR,P=0.049) and homozygous mutant (QQ versus RR,P=0.004) were significantly associated with amoebic liver abscess when compared with homozygous wild (QQ).

scholarly journals Epidemiology, haematology and molecular characterization of haemoprotozoon and rickettsial organisms causing infections in cattle of Jammu region, North India

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Rabjot Kaur ◽  
Anish Yadav ◽  
Shafiya I. Rafiqi ◽  
Rajesh Godara ◽  
Vikrant Sudan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genetic Characterization ◽  
Disease Mapping ◽  
Anaplasma Marginale ◽  
Theileria Annulata ◽  
Genomic Diversity ◽  
North India ◽  
Babesia Bigemina ◽  
Prevalence Studies

Abstract Background The present study was aimed at establishing the prevalence, epidemiology and molecular characterization of major haemoprotozoons (Babesia and Theileria) and rickettsia (Anaplasma) of cattle in Jammu region (North India) using microscopy and Polymerase Chain Reaction (PCR). Hematology, microscopy and PCR based prevalence studies were undertaken with 278 whole blood samples from cattle. Molecular prevalence studies were followed by genetic characterization of the isolates of Babesia, Anaplasma and Theileria spp. based on 18S rRNA, 16S rRNA and Tams1 gene, respectively. The data related to metrology and epidemiological variables like temperature, rainfall, season, age and type of livestock rearing was analyzed and correlated with occurrence of disease by statistical methods. Results The prevalence based on microscopy was 12.9% (36/278) whereas PCR recorded 30.22% (84/278) animals positive for haemoparasitic infections. All the samples found positive by microscopy were also recorded positive by PCR. Thus the study revealed prevalence of Babesia bigemina, Anaplasma marginale and Theileria annulata to be 9.7, 16.5 and 0.7% respectively. The metrological and epidemiological variables made inroads for the propagation of vector ticks and occurrence of infection. Haematological alterations predominantly related to decrease in haemoglobin, red blood cell count and packed cell volume were evident in diseased animals and collaterally affected the productivity. Further the genetic characterization of Babesia bigemina. (MN566925.1, MN567603, MN566924.1), Anaplasma marginale. (MH733242.1, MN567602.1) and Theileria annulata (MT113479) provided a representative data of the isolates circulating in the region and their proximity with available sequences across the world. Conclusions Despite holding much significance to the animal sector, comprehensive disease mapping has yet not been undertaken in several parts of India. The present study provides a blue print of disease mapping, epidemiological correlations and genomic diversity of Babesia bigemina, Anaplasma marginale and Theileria annulata circulating in the region.

Detection of Theileria annulata by the PCR-RFLP in ticks (Acari, Ixodidae) collected from cattle in West and North-West Iran

2011 ◽  
Vol 56 (1) ◽  
Author(s):  
Mosa Tavassoli ◽  
Mohammad Tabatabaei ◽  
Bijan Nejad ◽  
Mehran Tabatabaei ◽  
Amin Najafabadi ◽  
...  
Keyword(s):  
Restriction Enzymes ◽  
Theileria Annulata ◽  
Gene Encoding ◽  
Hyalomma Anatolicum Anatolicum ◽  
North West ◽  
Blood Smears ◽  
Pcr Rflp ◽  
Single Pattern ◽  
Pcr Products ◽  
Bovine Theileriosis

AbstractThe presence of potential vectors, ticks, and susceptible hosts of bovine malignant theileriosis in all parts of Iran pose a real threat to food animal industry. The present study was conducted to determine the infection rate of ticks collected from naturally occurring bovine theileriosis in West and North-West Iran. Two hundred and thirty seven cattle suspected of suffering from theileriosis were investigated for the presence of Theileria annulata in the blood smears and any tick species on their body. In this study, 402 ticks were obtained from 99 cattle. The examination of 402 ticks by polymerase chain reaction (PCR) using primers derived from the gene encoding heat shock protein70 (Hsp70) revealed that 39.9% of Hyalomma anatolicum anatolicum, 3.5% of H. asiaticum asiaticum, and 18.2% H. anatolicum excavatum, were infected with T. annulata. The results suggest that H. a. anatolicum may play a major role in transmission of T. annulata infection in Iran. Finally, digestion of the PCR products of T. annulata with two different restriction enzymes produced only a single pattern.

scholarly journals Rickettsia helvetica in Ixodes ricinus Ticks in Sweden

1999 ◽  
Vol 37 (2) ◽  
pp. 400-403 ◽  
Author(s):  
Kenneth Nilsson ◽  
Olle Lindquist ◽  
Ai Jie Liu ◽  
Thomas G. T. Jaenson ◽  
Göran Friman ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Citrate Synthase ◽  
Spotted Fever ◽  
Oligonucleotide Primer ◽  
Rrna Gene ◽  
Spotted Fever Group ◽  
Rflp Pattern ◽  
Rickettsia Helvetica ◽  
Pcr Rflp ◽  
Ixodes Ricinus Ticks

In the present study further characterization of the amplified sequence of the citrate synthase gene of the spotted fever groupRickettsia isolated from Ixodes ricinus ticks in Sweden showed that it has 100% homology with the deposited sequence of the citrate synthase gene of Rickettsia helvetica. The restriction fragment length polymorphism (RFLP) pattern of an amplified 382-bp product of the citrate synthase sequence, defined by primers RpCS877 and RpCS1258, yielded fragments for our isolate that could be visualized as a double band that migrated at approximately 44 bp, another double band at 85 bp, and a single band at nearly 120 bp after digestion with the restriction enzyme AluI. When calculating a theoretical PCR-RFLP pattern of the sequence of the citrate synthase gene of R. helvetica from the known positions where the AluI enzyme cuts, we arrived at the same pattern that was obtained for our isolate, a pattern distinctly different from the previously published PCR-RFLP pattern for R. helvetica. Investigation of 125 living I. ricinusticks showed a higher prevalence of rickettsial DNA in these ticks than we had found in an earlier study. Rickettsial DNA was detected by amplification of the 16S rRNA gene, for which a seminested primer system consisting of two oligonucleotide primer pairs was used. Of the 125 ticks, some were pooled, giving a total of 82 tick samples, of which 20 were found to be positive for the rickettsial DNA gene investigated. When considering the fact that some of the positive samples were pooled, the minimum possible prevalence in these ticks was 20 of 125 (16%) and the maximum possible prevalence was 46 of 125 (36.8%). These prevalence estimates conform to those of other studies of spotted fever group rickettsiae in hard ticks in Europe.

Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR–RFLP

2010 ◽  
Vol 108 (4) ◽  
pp. 837-843 ◽  
Author(s):  
Mahdieh Zaeemi ◽  
Hamidreza Haddadzadeh ◽  
Parvaneh Khazraiinia ◽  
Bahram Kazemi ◽  
M. Bandehpour
Keyword(s):  
Nested Pcr ◽  
Infected Sheep ◽  
Theileria Annulata ◽  
Theileria Lestoquardi ◽  
Pcr Rflp

Molecular genetic characterization of the cervid strain (‘northern form’) ofEchinococcus granulosus

Parasitology ◽  
1994 ◽  
Vol 109 (2) ◽  
pp. 215-221 ◽  
Author(s):  
J. Bowles ◽  
D. Blair ◽  
D. P. McManus
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Rflp Analysis ◽  
Domestic Sheep ◽  
Rflp Pattern ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Mitochondrial Nd1 ◽  
Molecular Genetic Characterization

SUMMARYWe have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) ofEchinococcus granulosus.PCR–RFLP analysis of the nuclear ITS1 region of the rDNA repeat can readily distinguish the cervid form from other strains ofE. granulosus. The complexity of the RFLP patterns obtained suggests that a number of distinct ITS1 types are present in this strain which may represent an inter-strainE. granulosushybrid. Mitochondrial CO1 sequence of the cervid genotype was ambiguous at 18 positions and closely resembles a cluster of previously characterizedE. granulosusgenotypes, G1 (common, domestic sheep)/G2 (Tasmanian sheep)/G3 (buffalo). In contrast, mitochondrial ND1 sequence, although unique, suggests that the cervid form is most similar to strains represented by the G6 (camel)/G7 (pig) genotypes. We assume that the CO1 and ND1 sequences obtained for the cervid genotype are linked in a single mitochondrial genome although this is difficult to explain if conventional molecular genetics of mitochondrial DNA are assumed. Based on its unique ND1 sequence and ITS1 PCR–RFLP pattern, the cervid strain appears to represent a distinct genotype (designated G8) ofE. granulosus.

scholarly journals Association of TNF-α rs-281865419 polymorphism with reproductive tract infections in Indian population

2016 ◽  
Author(s):  
Vineeta Sharma ◽  
Pallavi Singhal ◽  
Anoop Kumar ◽  
V. G. Ramachandran ◽  
Shukla Das ◽  
...  
Keyword(s):  
Indian Population ◽  
Reproductive Tract ◽  
North India ◽  
Reproductive Tract Infections ◽  
Homozygous Genotype ◽  
Tnf Α ◽  
Pcr Rflp ◽  
Polymorphic Genotype ◽  
Tract Infections ◽  
Asymptomatic Women

Aim: To investigate the presence of reproductive tract infections (RTIs) in symptomatic and asymptomatic women in North India and association of SNPs in TNFα gene (rs-281865419 C/T) with susceptibility to these RTIs. Methods: We collected 100 symptomatic (cases) and 100 asymptomatic women (controls) samples and screened them for RTIs. Then genotyping of TNF-α gene was performed by PCR-RFLP. Results: Among cases the frequencies of RTIs infection is higher than control. The prevalence of HPV, C. trachomatis, T. vaginalis, Bacterial vaginosis and N. gonorrhoeae are 28% & 6%; 11%, 32% respectively while in controls it was 5%, 2%, 1% and 8% & 1%. In the present study we found that the frequency of wild homozygous genotype (TT) was lower in cases 30% (6/20) as compared to controls 60% (12/20). The frequency of the heterozygous polymorphic genotype (CT) was higher in cases 65% (65/100) as compared to controls 32% (32/100). It was interesting to note that the frequency of the polymorphic homozygous genotype (CC) was higher in cases 15% (15/100) than controls 2% (2/100). While the frequency of the carrier genotype (CT + TT) was found to be more in cases 70% (70/100) than in controls 40/100 (40%). This study shows that T allele may be risk factor for Reproductive tract infections as its percentage is higher in cases as compare to normal controls. Conclusion: TNF-? rs-281865419 locus may serve as an important biomarker for RTIs predisposition in Indian population though larger sample size is needed to validate the findings.

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