Micro-Surgical Fascicular Nerve Repair: A Morphological Study of the Endoneurial Bulge

1987 ◽  
Vol 12 (1) ◽  
pp. 5-10
Author(s):  
S. J. ARCHIBALD ◽  
T. R. FISHER
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sciatic Nerve ◽  
Morphological Study ◽  
Morphological Changes ◽  
Nerve Repair ◽  
Razor Blade ◽  
Rat Sciatic Nerve ◽  
Sharp Razor Blade ◽  
Scanning Electron

The phenomenon of ‘endoneurial bulging’ from the cut end of the divided nerve fasciculus in the rat sciatic nerve has been examined with light and scanning electron microscopy and the morphological changes compared when dividing the fasciculus with either micro-scissors or a sharp razor blade.

Micro-Surgical Fascicular Nerve Repair: A Morphological Study of the Endoneurial Bulge

1992 ◽  
Vol 17 (3) ◽  
pp. 375-377
Author(s):  
S. J. Archibald ◽  
T. R. Fisher
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sciatic Nerve ◽  
Morphological Study ◽  
Morphological Changes ◽  
Nerve Repair ◽  
Razor Blade ◽  
Rat Sciatic Nerve ◽  
Sharp Razor Blade ◽  
Scanning Electron

The phenomenon of ‘endoneurial bulging’ from the cut end of the divided nerve fasciculus in the rat sciatic nerve has been examined with light and scanning electron microscopy and the morphological changes compared when dividing the fasciculus with either micro-scissors or a sharp razor blade.

Field-Emission Scanning Electron Microscopy and Energy-Dispersive X-Ray Analysis to Understand the Role of Tannin-Based Dyes in the Degradation of Historical Wool Textiles

2014 ◽  
Vol 20 (5) ◽  
pp. 1534-1543 ◽  
Author(s):  
Annalaura Restivo ◽  
Ilaria Degano ◽  
Erika Ribechini ◽  
Josefina Pérez-Arantegui ◽  
Maria Perla Colombini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Elemental Composition ◽  
Field Emission ◽  
Cross Sections ◽  
Morphological Changes ◽  
Energy Dispersive ◽  
X Ray ◽  
Edx Analysis ◽  
Scanning Electron

Abstract:An innovative approach, combining field-emission scanning electron microscopy (FESEM) with energy dispersive X-ray spectroscopy (EDX) analysis, is presented to investigate the degradation mechanisms affecting tannin-dyed wool. In fact, tannin-dyed textiles are more sensitive to degradation then those dyed with other dyestuffs, even in the same conservation conditions.FESEM-EDX was first used to study a set of 48 wool specimens (artificially aged) dyed with several raw materials and mordants, and prepared according to historical dyeing recipes. EDX analysis was performed on the surface of wool threads and on their cross-sections. In addition, in order to validate the model formulated by the analysis of reference materials, several samples collected from historical and archaeological textiles were subjected to FESEM-EDX analysis.FESEM-EDX investigations enabled us to reveal the correlation between elemental composition and morphological changes. In addition, aging processes were clarified by studying changes in the elemental composition of wool from the protective cuticle to the fiber core in cross-sections. Morphological and elemental analysis of wool specimens and of archaeological and historical textiles showed that the presence of tannins increases wool damage, primarily by causing a sulfur decrease and fiber oxidation.

scholarly journals Effect of Nerve-Cutting Technique on Nerve Microstructure and Neuroma Formation

2018 ◽  
Vol 11 (01) ◽  
pp. 028-034
Author(s):  
Wayne Rummings ◽  
P. Honeycutt ◽  
Edward Jernigan ◽  
Paul Weinhold ◽  
Reid Draeger
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sciatic Nerve ◽  
Clinical Importance ◽  
Sprague Dawley ◽  
Tongue Depressor ◽  
Cutting Guide ◽  
Scalpel Blade ◽  
Neuroma Formation ◽  
Scanning Electron

Abstract Background Peripheral neuroma formation results from partial or complete nerve division. Elucidating measures to prevent the development of peripheral neuromas is of clinical importance. The aim of this study was to determine the effect of various surgical nerve-cutting techniques on nerve microstructure and resultant neuroma formation. Methods Twenty Sprague-Dawley rats were randomly assigned to one of the following nerve-cutting techniques: No. 15 scalpel blade with tongue depressor, micro-serrated scissors, nerve-cutting guide forceps with straight razor, and bipolar cauterization. The right sciatic nerve was transected using the assigned nerve-cutting technique. Neuromas were harvested 6 weeks postoperatively, and samples were obtained for histologic analysis. The contralateral sciatic nerve was transected at euthanasia and analyzed with histology and with scanning electron microscopy in a subset of the rats. Results Fifteen of the 20 rats survived the 6-week experiment. Scanning electron microscopy of the No. 15 scalpel blade group showed the most visual damage and disorganization whereas the nerve-cutting guide forceps and micro-serrated scissors groups resulted in a smooth transected surface. Bipolar cauterization appeared to enclose the fascicular architecture within a sealed epineurium. Each neuroma was significantly larger than contralateral controls. There were no significant differences in neuroma caliber between nerve transection groups. No substantial differences in microstructure were evident between transection groups. Conclusion Despite disparate microscopic appearances of the cut surfaces of nerves using various nerve-cutting techniques, we found no significant differences in the caliber or incidence of neuroma formation based on nerve-cutting technique. Nerve-cutting technique used when transecting peripheral nerves may have little bearing on the formation or size of resultant neuroma formation.

scholarly journals Rapid induction of morphological changes in human carcinoma cells A-431 by epidermal growth factors.

1979 ◽  
Vol 83 (1) ◽  
pp. 260-265 ◽  
Author(s):  
M Chinkers ◽  
J A McKanna ◽  
S Cohen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Growth Factors ◽  
Morphological Changes ◽  
Carcinoma Cells ◽  
Human Carcinoma ◽  
Rapid Induction ◽  
Epidermal Growth ◽  
Scanning Electron

The morphological effects of epidermal growth factor (EGF) on human carcinoma cells A-431 have been examined by scanning electron microscopy. These flat polygonal cells normally exhibit only small membrane folds, but show extensive ruffling and extension of filopodia within 5 min of exposure to EGF at 37 degrees C. This ruffling activity is transient, subsiding within another 5--15 min, but several other changes in surface morphology follow. Within the first hour of exposure to the hormone, the cell surface becomes exceedingly smooth and the nuclei seem to protrude above the plane of the otherwise thin monolayer, giving the cells a "fried egg" appearance. Cells at the edges of colonies gradually retract from the substrate, leading to reorganization, by 12 h, of the monolayer into multilayered colonies. EGF thus induces both rapid and long-term alterations in the morphology of these epidermoid cells.

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