Relationship between structure and function of liver mitochondria from hibernating and active ground squirrels, Citellus undulatus

1993 ◽  
Vol 106 (1) ◽  
pp. 125-130 ◽  
Author(s):  
N.N. Brustovetsky ◽  
M.V. Egorova ◽  
E.N. Iljasova ◽  
L.E. Bakeeva
Keyword(s):  
Liver Mitochondria ◽  
Structure And Function ◽  
Ground Squirrels ◽  
And Function

Irreversible hemorrhagic shock in dogs: structure and function of liver mitochondria

1961 ◽  
Vol 200 (2) ◽  
pp. 264-268 ◽  
Author(s):  
H. Hift ◽  
J. G. Strawitz
Keyword(s):  
Hemorrhagic Shock ◽  
Membrane Structure ◽  
Phase Contrast ◽  
Krebs Cycle ◽  
Liver Mitochondria ◽  
Structure And Function ◽  
Absorbing Material ◽  
Krebs Cycle Enzymes ◽  
And Function ◽  
Normal Controls

Paired experiments were conducted with dogs dying in irreversible hemorrhagic shock and with normal controls. Examined under the electron microscope the liver cells from the experimental dogs were found to contain enlarged mitochondria characterized by a swollen matrix, rather than an altered double-membrane structure. Swollen, abnormal particles were also observed under phase contrast in whole-tissue blendorates and purified mitochondrial suspensions derived from such livers. Simultaneously, slices and purified suspensions prepared from those livers yielded higher than normal oxygen uptakes but normal P/O ratios and the purified mitochondria were found to contain normal proportions but excessive amounts of proteinous, nitrogenous and UV-absorbing material. It is suggested that liver mitochondria adjust to the stress imposed by the prolonged oligemia and hypoxia by enlarging and acquiring an extra complement of Krebs' cycle enzymes. The liver may thus be in a state of potential hyperactivity at a time when the animal as a whole is succumbing to the effects of irreversible hemorrhagic shock.

The Effects of Hypophysectomy on the Structure and Function of Rat Liver Mitochondria

1972 ◽  
Vol 72 (6) ◽  
pp. 1379-1390 ◽  
Author(s):  
Takashi MATSUBARA ◽  
Toshiaki HASEGAWA ◽  
Yoshihiro TOCHINO ◽  
Akira TANAKA ◽  
Yoshiko UEMURA-ISHIKAWA ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Structure And Function ◽  
Rat Liver Mitochondria ◽  
And Function

scholarly journals Effect of the Lipophilic o-Naphthoquinone CG 10-248 on Rat Liver Mitochondria Structure and Function

BIOCELL ◽  
2002 ◽  
Vol 26 (2) ◽  
pp. 237-245 ◽  
Author(s):  
LIDIA M. LOPE ◽  
AMANDA PELLEGRINO DE IRALDI ◽  
PATRICIA H. CARRIZO ◽  
MARTA DUBIN ◽  
ANDR蒘 O.M. STOPPANI
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Structure And Function ◽  
Rat Liver Mitochondria ◽  
And Function

Effect of hibernation and jejunal bypass on mucosal structure and function

1991 ◽  
Vol 261 (1) ◽  
pp. G37-G44 ◽  
Author(s):  
H. V. Carey ◽  
H. J. Cooke
Keyword(s):  
Animal Model ◽  
Structure And Function ◽  
Ground Squirrels ◽  
Oral Feeding ◽  
Factors Associated ◽  
Local Responses ◽  
Systemic Factors ◽  
Mucosal Mass ◽  
And Function ◽  
Jejunal Bypass

Intestinal mucosal structure and function may be regulated by systemic factors associated with oral feeding, as well as local responses initiated by contact of the mucosa with food. This study compared the relative effects of these factors in an animal model that undergoes seasonal long-term fasting. Jejunal bypass operations or sham surgeries were performed on active fed ground squirrels or on squirrels that subsequently ceased feeding and hibernated. Mucosal wet weight, protein content, villus height, and surface area were reduced in jejunal segments that had minimal exposure to luminal contents (bypassed segments of active squirrels and all segments of hibernators) compared with segments exposed to the luminal stream (incontinuity and sham segments of active squirrels). When normalized to mucosal weight, transepithelial absorption of 3-O-methylglucose and alanine-dependent sodium flux were greater in jejunal segments with minimal exposure to luminal nutrients. Altered structure in bypassed segments of active and hibernating squirrels paralleled changes in functional parameters despite the presence of different systemic factors in the two groups. Thus, in this animal model, contact of the mucosa with food, and not systemic factors associated with oral feeding, is the primary factor maintaining mucosal mass. The absence of mucosal contact with nutrients enhances specific absorptive function by mechanisms that have yet to be determined.

Structure and function of neural networks in the retina

1988 ◽  
Vol 46 ◽  
pp. 26-27
Author(s):  
Peter Sterling
Keyword(s):  
Ganglion Cells ◽  
Three Dimensional ◽  
Structure And Function ◽  
Synaptic Connections ◽  
Visual Axis ◽  
One Dimensional ◽  
Cat Retina ◽  
Ultrathin Sections ◽  
And Function ◽  
Insight Into

The synaptic connections in cat retina that link photoreceptors to ganglion cells have been analyzed quantitatively. Our approach has been to prepare serial, ultrathin sections and photograph en montage at low magnification (˜2000X) in the electron microscope. Six series, 100-300 sections long, have been prepared over the last decade. They derive from different cats but always from the same region of retina, about one degree from the center of the visual axis. The material has been analyzed by reconstructing adjacent neurons in each array and then identifying systematically the synaptic connections between arrays. Most reconstructions were done manually by tracing the outlines of processes in successive sections onto acetate sheets aligned on a cartoonist's jig. The tracings were then digitized, stacked by computer, and printed with the hidden lines removed. The results have provided rather than the usual one-dimensional account of pathways, a three-dimensional account of circuits. From this has emerged insight into the functional architecture.

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

Use of human autoantibodies and immunoelectron microscopy to study structure and function of the nucleolus and nuclear bodies

1992 ◽  
Vol 50 (1) ◽  
pp. 506-507
Author(s):  
Robert L. Ochs
Keyword(s):  
Electron Microscopy ◽  
Structure And Function ◽  
Nuclear Bodies ◽  
Nuclear Interior ◽  
Coiled Bodies ◽  
Interchromatin Granules ◽  
And Function ◽  
Conventional Electron Microscopy ◽  
Perichromatin Granules ◽  
Perichromatin Fibrils

By conventional electron microscopy, the formed elements of the nuclear interior include the nucleolus, chromatin, interchromatin granules, perichromatin granules, perichromatin fibrils, and various types of nuclear bodies (Figs. 1a-c). Of these structures, all have been reasonably well characterized structurally and functionally except for nuclear bodies. The most common types of nuclear bodies are simple nuclear bodies and coiled bodies (Figs. 1a,c). Since nuclear bodies are small in size (0.2-1.0 μm in diameter) and infrequent in number, they are often overlooked or simply not observed in any random thin section. The rat liver hepatocyte in Fig. 1b is a case in point. Historically, nuclear bodies are more prominent in hyperactive cells, they often occur in proximity to nucleoli (Fig. 1c), and sometimes they are observed to “bud off” from the nucleolar surface.

Depletion and Reconstitution of Active E. Coli Ribosomes

1975 ◽  
Vol 33 ◽  
pp. 640-641
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Protein Biosynthesis ◽  
Large Subunit ◽  
High Resolution Electron Microscopy ◽  
Small Subunit ◽  
Structure And Function ◽  
Biologically Active ◽  
Biological Macromolecules ◽  
E Coli ◽  
Resolution Electron ◽  
And Function

Correlations between structure and function of biological macromolecules have been studied intensively for many years, mostly by indirect methods. High resolution electron microscopy is a unique tool which can provide such information directly by comparing the conformation of biopolymers in their biologically active and inactive state. We have correlated the structure and function of ribosomes, ribonucleoprotein particles which are the site of protein biosynthesis. 70S E. coli ribosomes, used in this experiment, are composed of two subunits - large (50S) and small (30S). The large subunit consists of 34 proteins and two different ribonucleic acid molecules. The small subunit contains 21 proteins and one RNA molecule. All proteins (with the exception of L7 and L12) are present in one copy per ribosome.This study deals with the changes in the fine structure of E. coli ribosomes depleted of proteins L7 and L12. These proteins are unique in many aspects.

The Laryngeal Epithelium in Reflux

2011 ◽  
Vol 21 (3) ◽  
pp. 112-117 ◽  
Author(s):  
Elizabeth Erickson-Levendoski ◽  
Mahalakshmi Sivasankar
Keyword(s):  
Laryngopharyngeal Reflux ◽  
Critical Role ◽  
Structure And Function ◽  
Laryngeal Disease ◽  
Health And Disease ◽  
And Function ◽  
The Impact

The epithelium plays a critical role in the maintenance of laryngeal health. This is evident in that laryngeal disease may result when the integrity of the epithelium is compromised by insults such as laryngopharyngeal reflux. In this article, we will review the structure and function of the laryngeal epithelium and summarize the impact of laryngopharyngeal reflux on the epithelium. Research investigating the ramifications of reflux on the epithelium has improved our understanding of laryngeal disease associated with laryngopharyngeal reflux. It further highlights the need for continued research on the laryngeal epithelium in health and disease.

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