Isolation and characterization of the gene coding for the major sigma factor of Rickettsia prowazekii DNA-dependent RNA polymerase

Gene ◽  
1992 ◽  
Vol 121 (1) ◽  
pp. 155-160 ◽  
Author(s):  
G.Lynn Marks ◽  
Herbert H. Winkler ◽  
David O. Wood
Keyword(s):  
Rna Polymerase ◽  
Sigma Factor ◽  
Rickettsia Prowazekii ◽  
Isolation And Characterization ◽  
Gene Coding

scholarly journals Isolation and characterization of the hypA gene coding for HU of Aeromonar Proteolytica

1992 ◽  
Vol 20 (15) ◽  
pp. 4092-4092 ◽  
Author(s):  
Hilla Giladi ◽  
Wei-Xian Wang ◽  
Amos B. Oppenheim
Keyword(s):  
Isolation And Characterization ◽  
Gene Coding

scholarly journals Isolation and Characterization of the Gene Coding for the Amidinotransferase Involved in the Biosynthesis of Phaseolotoxin in Pseudomonas syringae pv. phaseolicola

2001 ◽  
Vol 14 (4) ◽  
pp. 545-554 ◽  
Author(s):  
Gustavo Hernández-Guzmán ◽  
Ariel Alvarez-Morales
Keyword(s):  
Aspartic Acid ◽  
Pseudomonas Syringae ◽  
Sequence Similarity ◽  
Insertion Mutant ◽  
Halo Blight ◽  
Streptomyces Spp ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Blight Disease

Pseudomonas syringae pv. phaseolicola is the causal agent of the “halo blight” disease of beans. A key component in the development of the disease is a nonhost-specific toxin, Nδ-(N'-sulphodiaminophosphinyl)-ornithyl-alanyl-homoarginine, known as phaseolotoxin. The homoarginine residue in this molecule has been suggested to be the product of Larginine:lysine amidinotransferase activity, previously detected in extracts of P. syringae pv. phaseolicola grown under conditions of phaseolotoxin production. We report the isolation and characterization of an amidinotransferase gene (amtA) from P. syringae pv. phaseolicola coding for a polypeptide of 362 residues (41.36 kDa) and showing approximately 40% sequence similarity to Larginine:inosamine-phosphate amidinotransferase from three species of Streptomyces spp. and 50.4% with an Larginine:glycine amidinotransferase from human mitochondria. The cysteine, histidine, and aspartic acid residues involved in substrate binding are conserved. Furthermore, expression of the amtA and argK genes and phaseolotoxin production occurs at 18°C but not at 28°C. An amidinotransferase insertion mutant was obtained that lost the capacity to synthesize homoarginine and phaseolotoxin. These results show that the amtA gene isolated is responsible for the amidinotransferase activity detected previously and that phaseolotoxin production depends upon the activity of this gene.

scholarly journals Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.

1988 ◽  
Vol 170 (8) ◽  
pp. 3640-3649 ◽  
Author(s):  
Y N Zhou ◽  
N Kusukawa ◽  
J W Erickson ◽  
C A Gross ◽  
T Yura
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Sigma Factor ◽  
Isolation And Characterization ◽  
Sigma 32

scholarly journals Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (GTP) from the rat.

1983 ◽  
Vol 80 (12) ◽  
pp. 3656-3660 ◽  
Author(s):  
H. Yoo-Warren ◽  
J. E. Monahan ◽  
J. Short ◽  
H. Short ◽  
A. Bruzel ◽  
...  
Keyword(s):  
Phosphoenolpyruvate Carboxykinase ◽  
Isolation And Characterization ◽  
Gene Coding
Sign in / Sign up

Export Citation Format

Share Document