scholarly journals Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinase (GTP) from the rat.

1983 ◽  
Vol 80 (12) ◽  
pp. 3656-3660 ◽  
Author(s):  
H. Yoo-Warren ◽  
J. E. Monahan ◽  
J. Short ◽  
H. Short ◽  
A. Bruzel ◽  
...  
Keyword(s):  
Phosphoenolpyruvate Carboxykinase ◽  
Isolation And Characterization ◽  
Gene Coding

scholarly journals Isolation and characterization of the hypA gene coding for HU of Aeromonar Proteolytica

1992 ◽  
Vol 20 (15) ◽  
pp. 4092-4092 ◽  
Author(s):  
Hilla Giladi ◽  
Wei-Xian Wang ◽  
Amos B. Oppenheim
Keyword(s):  
Isolation And Characterization ◽  
Gene Coding

scholarly journals Isolation and Characterization of the Gene Coding for the Amidinotransferase Involved in the Biosynthesis of Phaseolotoxin in Pseudomonas syringae pv. phaseolicola

2001 ◽  
Vol 14 (4) ◽  
pp. 545-554 ◽  
Author(s):  
Gustavo Hernández-Guzmán ◽  
Ariel Alvarez-Morales
Keyword(s):  
Aspartic Acid ◽  
Pseudomonas Syringae ◽  
Sequence Similarity ◽  
Insertion Mutant ◽  
Halo Blight ◽  
Streptomyces Spp ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Blight Disease

Pseudomonas syringae pv. phaseolicola is the causal agent of the “halo blight” disease of beans. A key component in the development of the disease is a nonhost-specific toxin, Nδ-(N'-sulphodiaminophosphinyl)-ornithyl-alanyl-homoarginine, known as phaseolotoxin. The homoarginine residue in this molecule has been suggested to be the product of Larginine:lysine amidinotransferase activity, previously detected in extracts of P. syringae pv. phaseolicola grown under conditions of phaseolotoxin production. We report the isolation and characterization of an amidinotransferase gene (amtA) from P. syringae pv. phaseolicola coding for a polypeptide of 362 residues (41.36 kDa) and showing approximately 40% sequence similarity to Larginine:inosamine-phosphate amidinotransferase from three species of Streptomyces spp. and 50.4% with an Larginine:glycine amidinotransferase from human mitochondria. The cysteine, histidine, and aspartic acid residues involved in substrate binding are conserved. Furthermore, expression of the amtA and argK genes and phaseolotoxin production occurs at 18°C but not at 28°C. An amidinotransferase insertion mutant was obtained that lost the capacity to synthesize homoarginine and phaseolotoxin. These results show that the amtA gene isolated is responsible for the amidinotransferase activity detected previously and that phaseolotoxin production depends upon the activity of this gene.

scholarly journals Isolation and characterization of regulatory mutants from Schizosaccharomyces pombe involved in thiamine-regulated gene expression.

Genetics ◽  
1992 ◽  
Vol 130 (3) ◽  
pp. 445-449
Author(s):  
A M Schweingruber ◽  
H Fankhauser ◽  
J Dlugonski ◽  
C Steinmann-Loss ◽  
M E Schweingruber
Keyword(s):  
Acid Phosphatase ◽  
Schizosaccharomyces Pombe ◽  
Mrna Levels ◽  
Wild Type ◽  
Transcription Control ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Thiamine Transport ◽  
Regulated Gene Expression

Abstract Mutants from Schizosaccharomyces pombe deficient in the regulation of thiamine-repressible acid phosphatase have been isolated. Mutants expressing derepressed levels of the enzyme in the presence and absence of thiamine map in three genes, tnr1, tnr2 and tnr3. mRNA levels of the pho4 gene (coding for thiamine repressible acid phosphatase) and another thiamine-regulatable gene, thi3 (coding for a thiamine biosynthetic enzyme and corresponding to nmt1) are constitutively synthesized in the mutants. The mutants also exhibit constitutive thiamine transport which is thiamine repressible in wild type. The tnr3 mutants reveal a 10-20-fold higher intracellular thiamine level than tnr1 and tnr2 mutants and wild type. Mutants expressing repressed levels of thiamine-repressible acid phosphatase map in gene thi1. No or little amounts of pho4- and nmt1-specific mRNA can be detected. These mutants are impaired in thiamine uptake and are thiamine auxotrophic due to the inability to synthesize the thiazole moiety of the thiamine molecule. All tested tnr and thi1 alleles are recessive, and thi1 mutations are epistatic over tnr mutations. We assume that the thi1 and tnr genes are involved in thiamine-mediated transcription control.

Isolation and characterization of the gene coding for murine high-mobility-group protein HMGI-C

Gene ◽  
1995 ◽  
Vol 167 (1-2) ◽  
pp. 249-253 ◽  
Author(s):  
Guidalberto Manfioletti ◽  
Alessandra Rustighi ◽  
Fiamma Mantovani ◽  
Graham H. Goodwin ◽  
Vincenzo Giancotti
Keyword(s):  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Group Protein

Isolation and characterization of a Saccharomycopsis lipolytica mutant showing increased production of citric acid from canola oil

1985 ◽  
Vol 31 (5) ◽  
pp. 436-440 ◽  
Author(s):  
David W. Good ◽  
Randal Droniuk ◽  
G. Ross Lawford ◽  
Jared E. Fein
Keyword(s):  
Citric Acid ◽  
Canola Oil ◽  
Acid Production ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Citric Acid Production ◽  
Isocitric Acid ◽  
Isolation And Characterization ◽  
Saccharomycopsis Lipolytica ◽  
Acid Ratio

A process for the production of citric acid from canola (rapeseed) oil using the yeast Saccharomycopsis lipolytica was examined. A citrate nonutilizing strain, designated NTG9, which had an improved citric to isocitric acid ratio, was isolated after mutagenesis of S. lipolytica ATCC 20228 with nitrosoguanidine. Although the mutant grew well on canola oil, unlike the parent strain or a spontaneous revertant (JF2), it did not grow on glycolytic intermediates below glycerate-3-phosphate, amino acids, hexadecane, or yellow kerosene. The mutant was shown to be impaired in the gluconeogenic pathway because of a loss of phosphoenolpyruvate carboxykinase activity. A preliminary study of the effect of micronutrients on citric acid production by S. lipolytica NTG9 showed that manganese had a stimulatory effect on the process whereas zinc and iron were inhibitory. A revised growth medium was tested and found to increase citric acid production while decreasing that of isocitric acid.

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