Reversed-phase chromatography of phenylthiocarbamyl amino acid derivatives of physiological amino acids: an evaluation and a comparison with analysis by ion-exchange chromatography

1992 ◽  
Vol 574 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Andrew S. Feste
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Reversed Phase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Amino Acid Derivatives ◽  
Reversed Phase Chromatography ◽  
Derivatives Of

Effect of Deproteinating Agents (Picric Acid and Sulfosalicylic Acid) on Analysis for Free Amino Acids in Swine Blood and Tissue

1969 ◽  
Vol 52 (5) ◽  
pp. 981-984 ◽  
Author(s):  
J E Knipfel ◽  
D A Christensen ◽  
B D Owen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino ◽  
Biological Fluids ◽  
Picric Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sulfosalicylic Acid ◽  
Coefficients Of Variation

Abstract Amino acid analyses were performed on samples of blood, liver tissue, loin muscle, and ham muscle by ion exchange chromatography after deproteination of the samples with picric acid or sulfosalicylic acid (SSA). Resolution of threonine and serine from the ion exchange column was poor when SSA was used as the deproteinating agent. Twelve of sixteen amino acids were higher (P < 0.05) in serum deproteinated with picric acid as compared to concentrations determined after SSA deproteination. Amino acid values for ham muscle tended to be higher after deproteination with picric acid; however, with liver and loin muscle samples, the values were somewhat higher after SSA deproteination. In both serum and tissue analyses, coefficients of variation were lower for niGSt amino acids when picric acid was utilized as the deproteinating agent. The latter observation, in particular, suggests that picric acid is preferable to SSA as a deproteinating agent before amino acid analyses of biological fluids. Standardization of methods of deproteination is needed to allow meaningful comparisons of data.

Ion exchange chromatography of 2-amino-2-deoxy-D-hexoses

1970 ◽  
Vol 48 (3) ◽  
pp. 386-388 ◽  
Author(s):  
M. Yaguchi ◽  
M. B. Perry
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Citrate Buffer ◽  
Neutral Amino Acids ◽  
Amino Acid Analyzer

An amino acid analyzer is used to separate seven 2-ammo-2-deoxy-D-hexoses (allosamine, altrosamine, glucosamine, mannosamine, gulosamine, galactosamine, and talosamine). A borate citrate buffer at pH 7.24 is used at 60 °C. Acidic and neutral amino acids, if present, are eluted before any of the hexosamines.

scholarly journals Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography

1992 ◽  
Vol 14 (4) ◽  
pp. 145-149 ◽  
Author(s):  
Frédéric Ziegler ◽  
Jacques Le Boucher ◽  
Colette Coudray-Lucas ◽  
Luc Cynober
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Reversed Phase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Reversed Phase Hplc ◽  
Suitable Alternative ◽  
Free Tryptophan ◽  
Rp Hplc ◽  
Low Concentrations

RP-HPLC with automated pre-column OPA derivatization is clearly a suitable alternative for assaying physiological AA and may be particularly useful for AA present at low concentrations (free tryptophan, plasma 3-methylhistidine).

scholarly journals Impairments of placental amino acid metabolism in fetal growth restriction

2017 ◽  
Vol 63 (3) ◽  
pp. 266-271 ◽  
Author(s):  
T.N. Pogorelova ◽  
V.O. Gunko ◽  
V.V. Avrutskaya ◽  
L.V. Kaushanskaya ◽  
O.A. Durnitsyna
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Growth Restriction

The content of the amino acids in the placenta during physiological pregnancy and fetal growth restriction (FGR) has been investigated my means of the method of ion-exchange chromatography. It has been found that in FGR the placental amino acid pool is characterized by a decreased content of arginine, proline, alanine, serine, cysteine, methionine, tryptophan, leucine, threonine, tyrosine, phenylalanine, glutamine and an increased content of dicarboxylic amino acids, lysine, histidine and glycine. These changes are accompanied by altered activity of some enzymes of amino acid metabolism, and the degree of these changes correlates with the level of corresponding amino acids.

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