Disc diffusion method to screen for high-level resistance to clindamycin and erythromycin in the Bacteroides fragilis group

1985 ◽  
Vol 3 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Donald R. Callihan ◽  
Frederick S. Nolte
Keyword(s):  
Bacteroides Fragilis ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacteroides Fragilis Group ◽  
High Level ◽  
Level Resistance

Detection of high- and moderately high-level resistance to gentamicin and streptomycin in Enterococcus faecium by a disc diffusion method

1995 ◽  
Vol 36 (1) ◽  
pp. 237-240
Author(s):  
H. Lopardo ◽  
C. Bantar ◽  
M. Venuta ◽  
L. Fernandez Canigia ◽  
S. Barbero ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
High Level ◽  
Level Resistance

scholarly journals Comparison of the Antimicrobial Susceptibility Results of Bacteroides fragilis Group Isolates Determined by Agar Dilution and the Tentative EUCAST Disk Diffusion Method

2021 ◽  
Author(s):  
Semra Eminoğlu ◽  
Bermal Tekeş ◽  
Elvan Sayın ◽  
Nurver Ülger Toprak
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bacteroides Fragilis ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacteroides Fragilis Group ◽  
Agar Dilution

Objective: In this study it was aimed to determine the antimicrobial susceptibility of Bacteroides fragilis group (BFG) bacteria using recently developed European Committee on Antimicrobial Susceptibility Testing (EUCAST) disc diffusion method and agar dilution method recommended by Clinical Laboratory Standart Institude (CLSI) for anaerobes and to investigate the agreement of the results of two tests. Method: The antimicrobial susceptibilities of a total of 56 BFG strains isolated from clinical samples and identified by MALDI-TOF MS analysis between January 2017 and December 2018, were tested to ampicillin, ampicillin/sulbactam, cefoxitin, imipenem, clindamycin, tigecycline, moxifloxacin and metronidazole MICs were determined by agar dilution method using sheep blood supplemented Brucella agar and disk diffusion test using host blood supplemented Fastidius Anaerobic Agar (FAA). Results: Six different BFG species consisting mostly strains of Bacteroides fragilis (n=34, 61%) and Bacteroides thetaiotaomicron (n=11, 20%) isolated from various clinical samples such as intraabdominal abscess (n= 24), blood (n=10) and tissue biopsy samples (n=11).were identified. Imipenem and metronidazole were the most effective antimicrobials with 98.2% susceptibility rates, followed by tigecycline, ampicillin/sulbactam, moxifloxacin and clindamycin with susceptibility rates of 89.3%, 66.1%, 57.1% and 46.4%, respectively. Most concordant results were obtained with metronidazole (100%), imipenem (89.8%) and tigecycline (89.8%). Acceptable compliance rates were not found for other antimicrobials. Conclusion: We can say that disc diffusion method is a fast, easy-to-apply, and reliable method used in clinical microbiology laboratories to determine the susceptibility of BFG bacteria to metronidazole, imipenem and tigecycline. However, to evolve a standard method especially for other antimicrobials, the experimental conditions should be optimized with studies dome with greater number of isolates.

Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong by the tentative EUCAST disc diffusion method

Anaerobe ◽  
2017 ◽  
Vol 47 ◽  
pp. 51-56 ◽  
Author(s):  
Pak-Leung Ho ◽  
Chong-Yee Yau ◽  
Lok-Yan Ho ◽  
Eileen Ling-Yi Lai ◽  
Melissa Chun-Jiao Liu ◽  
...  
Keyword(s):  
Hong Kong ◽  
Antimicrobial Susceptibility ◽  
Bacteroides Fragilis ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacteroides Fragilis Group

scholarly journals Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus

2007 ◽  
Vol 56 (7) ◽  
pp. 937-939 ◽  
Author(s):  
Naira Elane Moreira de Oliveira ◽  
Ana Paula Couto Marques Cardozo ◽  
Elizabeth de Andrade Marques ◽  
Kátia Regina Netto dos Santos ◽  
Marcia Giambiagi deMarval
Keyword(s):  
Staphylococcus Aureus ◽  
High Resistance ◽  
The Other ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Resistant Strains ◽  
Mupirocin Resistance ◽  
High Level

Meticillin-resistant Staphylococcus aureus isolates were classified into three mupirocin susceptibility groups by the disc diffusion method using 5 and 200 μg mupirocin discs. The zone diameter observed for a 5 μg disc distinguished MupS from the resistant strains (either MupRL or MupRH). On the other hand, a 200 μg disc distinguished the high-resistance MupRH strains from the other two (MupS or MupRL). Thus, the concomitant use of 5 and 200 μg mupirocin discs allowed the clear distinction among the three mupirocin susceptibility groups, MupS, MupRL or MupRH.

Antimicrobial susceptibility of Enterococcus spp. Isolated from different clinical sources in Kirkuk provency

2018 ◽  
pp. 97
Author(s):  
Hajir Ali Shareef ◽  
Shara Najmalddin Abdullah
Keyword(s):  
High Sensitivity ◽  
Diffusion Method ◽  
Multiple Resistance ◽  
Beta Lactamase ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Enterococcus Spp ◽  
New Antibiotics ◽  
Vitek 2 ◽  
High Level

        One of the most important problems confronts hospitals is the strains emergence  of Enterococcus spp. with multiple resistance to antibiotics, which propel researchers to modify or produce new antibiotics or combination between two antibiotics so that to be more effective against Enterococcus . This study was aimed to susceptibility some of local Enterococcus spp. Isolates with of 21 antibiotic using  disc diffusion method. The results showed absolute resistant 100% toward (Cephalexin , Gentamycin , Amikacin ,Erythromycin and Nalidixic acid), while showed a high sensitivity toward (Vancomycin and Impenem ) at percentage of 92.3% for each . Also highly inhibitory activity were observed by using penicillins antibiotics groups against most Enterococcus  isolates . which contribute to that non of the isolates showed it is ability to produce beta – lactamase enzymes by iodometric tube method. Also susceptibility to some new and synergetic antibiotic like Gentamicin High level(synergy), Streptomycin High level (synergy), Linezolid, Tigecycline, Levofloxacin, Quinupristin /Dalfopristin was conducted by Vitek-2 system. the results showed the absolute sensitivity (100%) of isolates toward ( Linezolid and Tigecycline).  All isolates showed multiple –resistant prescription to antibiotics , the number of antibiotics that every isolates resisted range between 6-12 antibiotic .

scholarly journals High level gentamicin resistance and susceptibility to Vancomycin in enterococci in a tertiary care hospital of Dhaka City

2014 ◽  
Vol 7 (2) ◽  
pp. 28-31 ◽  
Author(s):  
Shakila Tamanna ◽  
Lovely Barai ◽  
AA Ahmed ◽  
J Ashraful Haq
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Care Hospital ◽  
Dhaka City ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Agar Dilution ◽  
High Level

Vancomycin and high level gentamicin resistant enterococci detection is important for effective treatment and control of nosocomial infection. The present study was undertaken to determine the species distribution of Enterococcus and the rate of vancomycin and high level gentamicin resistant enterococci (HLGRE) in clinical samples in a tertiary care hospital of Dhaka city. Enterococci were identified to species level by standard biochemical and serological methods. Their susceptibilities to antibiotics were determined by disc diffusion method according to CLSI guideline. Minimum inhibitory concentration (MIC) of vancomycin and gentamicin were determined by agar dilution method. The study was conducted from July 2009 to February 2010. Among 80 isolates, 95% and 5% were identified as Enterococcus faecalis and Enterococcus faecium respectively. Out of 80 isolates 72 (90%) were sensitive and 8 (10%) were intermediate resistant to vancomycin (30?g) by disc diffusion method, but all isolates were susceptible by agar dilution MIC method. Out of 80 enterococci, 37 (46.25%) showed high level resistance to gentamicin (MIC: > 500 ?g/ml) by MIC method but, initially six of which showed sensitive result to gentamicin by disc diffusion method using 120 ?g disc. The study indicated high prevalence of HLGRE in our hospital population. MIC method was more accurate in detecting high level gentamycin resistant enterococci compared to disc diffusion method with 120 ?g gentamicin disc. However, none of the enterococcal strains showed resistance to vancomycin. HLGRE should be monitored regularly in clinical samples as it is difficult to treat. DOI: http://dx.doi.org/10.3329/imcj.v7i2.20103 Ibrahim Med. Coll. J. 2013; 7(2): 28-31

scholarly journals Control of Clinical Pathogens by the Haemolymph of Paratelphusa hydrodromous, a Freshwater Crab

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
A. Arul Prakash ◽  
S. Balasubramanian ◽  
G. Gunasekaran ◽  
M. Prakash ◽  
P. Senthil Raja
Keyword(s):  
Bacterial Species ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Freshwater Crab ◽  
Disc Diffusion Method ◽  
Strong Response ◽  
Clinical Pathogens ◽  
Rhizopus Sp ◽  
Antimicrobial Assay

In the present study, effort has been made to find the antimicrobial activity of haemolymph collected from freshwater crab, Paratelphusa hydrodromous. The haemolymph collected was tested for antimicrobial assay by disc diffusion method against clinical pathogens. Five bacterial species, namely, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and five fungal strains, namely and Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Rhizopus sp., and Mucor sp., were selected for the study. The result shows a strong response of haemolymph against the clinical pathogens which confirms the immune mechanism of the freshwater crab.

Selection and Substantiation of Methods for Evaluating Cosmetic Products for Deodorant and Antiperspirant Action

2021 ◽  
Vol 37 (2) ◽  
pp. 54-64
Author(s):  
D.V. Barabash ◽  
I.A. Butorova
Keyword(s):  
Large Scale ◽  
Gravimetric Method ◽  
Population Data ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Large Scale Testing ◽  
Sweat Secretion ◽  
Microbiological Methods ◽  
Number Of Microorganisms

The possibility of using simple and available methods for analyzing deodorants/antiperspirants has been studied. The gravimetric method was shown to have acceptable metrological characteristics under repeatability conditions when evaluating antiperspirant activity. A decrease in the number of microorganisms (CFU) on the axilla skin was observed in a rinse test experiment 4 h and 8 h after the application of deodorants/antiperspirants. The microbial population data were inversely proportional to the antiperspirant activity values of the tested compositions. The sweat secretion reducing decreases the amount of nutrients required for microbial development, which makes it possible to use the rinse test to indirectly evaluate deodorant activity in research and development of personal care products. However, due to its laboriousness and the need for volunteers, the method cannot be recommended for large-scale testing. It was shown that the disc diffusion method (DDM) used to detect Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis cannot be applied to the assessment of the intrinsic antimicrobial activity of the tested cosmetic compositions. This indicates the necessity of additional studies to select test microorganisms typical for the armpit area. In addition, DDM is useful if the deodorant effect of the composition is created by the addition of low-volatile antibacterial compounds. Therefore, microbiological methods have limited applications and are not suitable for widespread use. deodorant action; antiperspirant action, gravimetry, disc diffusion method, rinse test; deodorant; antiperspirant; cosmetic; efficiency; consumer properties, functional properties This work was supported by MUCTR (project no. K-2020-007).

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