scholarly journals A simple disc diffusion method for detecting AmpC and extended-spectrum  -lactamases in clinical isolates of Enterobacteriaceae

2009 ◽  
Vol 63 (3) ◽  
pp. 497-501 ◽  
Author(s):  
H. Derbyshire ◽  
G. Kay ◽  
K. Evans ◽  
C. Vaughan ◽  
U. Kavuri ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Extended Spectrum

scholarly journals ERROR RATE OF DISC DIFFUSION METHOD IN CEFTAZIDIME/ CEFOTAXIME SUSCEPTIBILITY TEST ON CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE (Laju Kesalahan Uji Kepekaan Ceftazidim/Cefotaxime Metode Difusi Cakram pada Klebsiella pneumoniae)

2018 ◽  
Vol 22 (3) ◽  
pp. 208
Author(s):  
Luz Maria GBW ◽  
Osman Sianipar ◽  
Usi Sukorini
Keyword(s):  
Klebsiella Pneumoniae ◽  
Error Rate ◽  
Susceptibility Test ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Major Error ◽  
Disc Diffusion Method ◽  
Minor Error ◽  
Extended Spectrum

Klebsiella pneumoniae merupakan salah satu bakteri Gram negatif yang banyak menimbulkan infeksi nosokomial. Difusi cakrammerupakan satu metode uji kepekaan antimikrobia yang banyak digunakan di laboratorium klinik yang juga dapat dipakai untuk menyaringK.pneumoniae penghasil enzim Extended-Spectrum β-Lactamase (ESBL). Antimikrobia pilihan untuk infeksi ini adalah cephalosporin generasiketiga (ceftazidime dan ceftotaxime). Penelitian ini bertujuan untuk mengetahui laju kesalahan uji kepekaan ceftazidime/cefotaxime metodedifusi cakram di isolat klinis K.peumoniae. Penelitian ini merupakan kajian potong lintang yang melibatkan 53 isolat klinis K.pneumoniae.Uji kepekaan ceftazidime/cefotaxime di isolat klinis K.pneumonia dilakukan menggunakan metode difusi cakram dan uji E sebagai rujukan.Hasil memeriksa dilaporkan dalam bentuk kepekaan, intermediate dan resistensi untuk setiap obat, dianalis untuk mengetahui laju kesalahan(minor error, major error dan very major error). Isolat klinis terbanyak berasal dari darah, air kemih dan nanah, berturut-turut 32,1%, 32,1%dan 18,9%. Sebagian besar isolat didapat dari ruang perawatan non-intensif (86,8%). Minor error uji kepekaan ceftazidime/cefotaximemetode difusi cakram berturut-turut 7,55% dan 1,89%, sehingga dapat disimpulkan bahwa metode difusi cakram uji kepekaan ceftazidime/cefotaxime dapat digunakan dalam uji kepekaan terhadap isolat klinik K.pneumoniae.

scholarly journals Beta Lactamase Genes of Extended Spectrum Beta Lactamase Producing Escherichia coli from Anorectal Sepsis Cases in Bangladesh

2016 ◽  
Vol 30 (1-2) ◽  
pp. 23-29
Author(s):  
Sheikh Shahidul Islam ◽  
Md Abdul Malek ◽  
AKM Fazlul Haque ◽  
Kaisar Ali Talukder ◽  
Marufa Zerin Akhter
Keyword(s):  
Multidrug Resistant ◽  
Diffusion Method ◽  
Pcr Analysis ◽  
Beta Lactamase ◽  
Disc Diffusion ◽  
Esbl Production ◽  
Disc Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

A microbiological study was carried out to determine the prevalence of extended spectrum beta lactamase (ESBL) producing E. coli in anorectal sepsis patients in Bangladesh. One hundred specimens of pus, swab, or exudates from anorectal sepsis cases were studied. All the 61 isolates of E. coli were found to be highly resistant to most of the drugs used. Among these, 14 multidrug resistant E. coli were examined for ESBL production by double disc diffusion method. Six of these were found to be ESBL positive. PCR analysis revealed that 3 of the 6 isolates had coexistence of blaSHV, blaOXA and blaCTXM-1 genes. Two of the isolates had only blaSHV gene, whereas 1 isolate had a combination of blaTEM and blaSHV genes. Three of these were resistant to all the drugs tested, while two were sensitive to getamicin and one to ciprofloxacin. None of the E. coli strains possessed blaCTXM-2, blaCTXM-8, blaCTXM-9, and qnr genes.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 23-29

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