Site-specific gene expression in vivo by direct gene transfer into the arterial wall

1993 ◽  
Vol 17 (3) ◽  
pp. 631-632
Author(s):  
Gregorio A. Sicard
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Arterial Wall ◽  
Specific Gene ◽  
Direct Gene Transfer ◽  
Site Specific ◽  
Specific Gene Expression ◽  
Direct Gene

scholarly journals Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain

FEBS Letters ◽  
1998 ◽  
Vol 433 (1-2) ◽  
pp. 37-40 ◽  
Author(s):  
Makoto Sawada ◽  
Fumihiro Imai ◽  
Hiromi Suzuki ◽  
Motoharu Hayakawa ◽  
Tetsuo Kanno ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Microglial Cell ◽  
Mammalian Brain ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals Site-specific gene expression analysis using an automated tissue micro-dissection punching system

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Takuya Yoda ◽  
Masahito Hosokawa ◽  
Kiyofumi Takahashi ◽  
Chikako Sakanashi ◽  
Haruko Takeyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Specific Gene ◽  
Site Specific ◽  
Specific Gene Expression

Laser microdissection as a new tool to investigate site-specific gene expression in enteric ganglia of the human intestine

2010 ◽  
Vol 22 (2) ◽  
pp. 168-e52 ◽  
Author(s):  
m. böttner ◽  
f. bär ◽  
h. von koschitzky ◽  
k. tafazzoli ◽  
u. j. roblick ◽  
...  
Keyword(s):  
Gene Expression ◽  
Laser Microdissection ◽  
Specific Gene ◽  
Human Intestine ◽  
Site Specific ◽  
Specific Gene Expression ◽  
Enteric Ganglia

scholarly journals Effects of Different Fixatives on β-Galactosidase Activity

2002 ◽  
Vol 50 (10) ◽  
pp. 1421-1424 ◽  
Author(s):  
Wenbin Ma ◽  
Keith Rogers ◽  
Berton Zbar ◽  
Laura Schmidt
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Specific Gene ◽  
Galactosidase Activity ◽  
Time Points ◽  
Specific Gene Expression ◽  
Carnoy’S Solution ◽  
Reporter Mice ◽  
Different Temperatures

β-Galactosidase (β-Gal) staining is widely used to demonstrate specific gene expression during evaluation of gene targets in vivo. This technique is extremely sensitive to fixation. Optimal fixation conditions are necessary to obtain the maximal β-Gal activity. In this experiment, Carnoy's and three different aldehyde fixatives were used at different temperatures and over different time points. Kidneys from LacZ-stop-human alkaline phosphatase (ZA/P) double reporter mice were used to generate positive material for the experiment. The results show that glutaraldehyde combinative solution (LacZ) produced the most consistent and reliable results. Paraformaldehyde and formaldehyde were effective as fixatives only at 4C for a period of less than 4 hr, and Carnoy's solution destroyed β-Gal activity.

scholarly journals Epigenetic regulation of neural cell differentiation plasticity in the adult mammalian brain

2008 ◽  
Vol 105 (46) ◽  
pp. 18012-18017 ◽  
Author(s):  
Jun Kohyama ◽  
Takuro Kojima ◽  
Eriko Takatsuka ◽  
Toru Yamashita ◽  
Jun Namiki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Target Genes ◽  
Epigenetic Modification ◽  
Mammalian Brain ◽  
Cytokine Signaling ◽  
Specific Gene ◽  
Neural Cells ◽  
Specific Gene Expression

Neural stem/progenitor cells (NSCs/NPCs) give rise to neurons, astrocytes, and oligodendrocytes. It has become apparent that intracellular epigenetic modification including DNA methylation, in concert with extracellular cues such as cytokine signaling, is deeply involved in fate specification of NSCs/NPCs by defining cell-type specific gene expression. However, it is still unclear how differentiated neural cells retain their specific attributes by repressing cellular properties characteristic of other lineages. In previous work we have shown that methyl-CpG binding protein transcriptional repressors (MBDs), which are expressed predominantly in neurons in the central nervous system, inhibit astrocyte-specific gene expression by binding to highly methylated regions of their target genes. Here we report that oligodendrocytes, which do not express MBDs, can transdifferentiate into astrocytes both in vitro (cytokine stimulation) and in vivo (ischemic injury) through the activation of the JAK/STAT signaling pathway. These findings suggest that differentiation plasticity in neural cells is regulated by cell-intrinsic epigenetic mechanisms in collaboration with ambient cell-extrinsic cues.

Tu-P7:3 Sex- and site-specific gene expression in the adipose tissue of OB/OB mice

2006 ◽  
Vol 7 (3) ◽  
pp. 185
Author(s):  
S. Shiozaki ◽  
T. Chiba ◽  
K. Kokame ◽  
T. Miyata ◽  
M. Ai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Specific Gene ◽  
Site Specific ◽  
Specific Gene Expression
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