Nucleoside/nucleotide pools in wheat leaves naturally infected with powdery mildew. II. Effects of leaf age and intensity of infection

1988 ◽  
Vol 33 (3) ◽  
pp. 507-514 ◽  
Author(s):  
A. Sawert ◽  
H.-J. Aust ◽  
K.G. Wagner
Plant Disease ◽  
2008 ◽  
Vol 92 (7) ◽  
pp. 1074-1082 ◽  
Author(s):  
Ryan Parks ◽  
Ignazio Carbone ◽  
J. Paul Murphy ◽  
David Marshall ◽  
Christina Cowger

Little is known about the population structure of wheat powdery mildew in the eastern United States, and the most recent report on virulence in this population involved isolates collected in 1993–94. In the present study, wheat leaves naturally infected with powdery mildew were collected from 10 locations in the southeastern United States in 2003 and 2005 and a collection of 207 isolates was derived from single ascospores. Frequencies of virulence to 16 mildew resistance (Pm) genes were determined by inoculating the isolates individually on replicated plates of detached leaves of differential wheat lines. These virulence frequencies were used to infer local effectiveness of Pm genes, estimate virulence complexity, detect significant associations between pairs of pathogen avirulence loci, and assess whether phenotypic differences between pathogen subpopulations increased with geographic distance. In both years, virulence to Pm3a, Pm3c, Pm5a, and Pm7 was present in more than 90% of sampled isolates and virulence to Pm1a, Pm16, Pm17, and Pm25 was present in fewer than 10% of isolates. In each year, 71 to 88% of all sampled isolates possessed one of a few multilocus virulence phenotypes, although there were significant differences among locations in frequencies of virulence to individual Pm genes. Several significant associations were detected between alleles for avirulence to pairs of Pm genes. Genetic (phenotypic) distance between isolate subpopulations increased significantly (R2 = 0.40, P < 0.001) with increasing geographic separation; possible explanations include different commercial deployment of Pm genes and restricted gene flow in the pathogen population.


Author(s):  
Alexander V. Babosha

Abscisic acid (ABA) plays an important role in the regulation of protective processes under stresses of various nature. In contrast to abiotic stresses, when a plant and a pathogen interact, this phytohormone is in most cases a negative regulator of resistance. However, even with a similar nature of pathogenesis, ABA can produce different effects. For example, ABA treatment in different experiments induced either a decrease or an increase in the susceptibility of cereals to powdery mildew. The aim of this work was to study the immunomodulatory properties of exogenous ABA depending on its concentration in the pathosystem composed of wheat Triticum aestivum L. plants and powdery mildew pathogen Blumeria graminis (DC.) Speer f. sp. tritici (syn. Erysiphe graminis). We studied the change in the number of pathogen colonies on susceptible wheat leaves (Zarya and Tavrichanka varieties) when two-week-old seedlings were treated with various ABA concentrations (0–9 μM) before and immediately after they were infected. When whole plants were used in the experiment, ABA was added to Knop’s solution; in experiments with detached leaves floating in Petri dishes, aqueous solutions of the phytohormone were used. Our results show that the magnitude and direction of the effect of exogenous ABA on the number of colonies of the pathogen depends on its concentration and the time of application relative to the moment of infection. ABA concentration dependence was variable in form: similar concentrations could be inhibitory, resulting in the minimum number of colonies, or stimulating, with the maximum number of colonies. At the same time, the pre-infection use of ABA was more likely to be inhibitory. The non-monotonicity and variation of the form of concentration dependence could probably account for the contradictory literature data on the immunomodulatory properties of ABA. The complex nature of the concentration dependence and the corresponding variation in the immunological state within a fairly wide range seem to ensure the Abscisic acid (ABA) plays an important role in the regulation of protective processes under stresses of various nature. In contrast to abiotic stresses, when a plant and a pathogen interact, this phytohormone is in most cases a negative regulator of resistance. However, even with a similar nature of pathogenesis, ABA can produce different effects. For example, ABA treatment in different experiments induced either a decrease or an increase in the susceptibility of cereals to powdery mildew. The aim of this work was to study the immunomodulatory properties of exogenous ABA depending on its concentration in the pathosystem composed of wheat Triticum aestivum L. plants and powdery mildew pathogen Blumeria graminis (DC.) Speer f. sp. tritici (syn. Erysiphe graminis). We studied the change in the number of pathogen colonies on susceptible wheat leaves (Zarya and Tavrichanka varieties) when two-week-old seedlings were treated with various ABA concentrations (0–9 μM) before and immediately after they were infected. When whole plants were used in the experiment, ABA was added to Knop’s solution; in experiments with detached leaves floating in Petri dishes, aqueous solutions of the phytohormone were used. Our results show that the magnitude and direction of the effect of exogenous ABA on the number of colonies of the pathogen depends on its concentration and the time of application relative to the moment of infection. ABA concentration dependence was variable in form: similar concentrations could be inhibitory, resulting in the minimum number of colonies, or stimulating, with the maximum number of colonies. At the same time, the pre-infection use of ABA was more likely to be inhibitory. The non-monotonicity and variation of the form of concentration dependence could probably account for the contradictory literature data on the immunomodulatory properties of ABA. The complex nature of the concentration dependence and the corresponding variation in the immunological state within a fairly wide range seem to ensure themaintenance of equilibrium in the pathosystem and the chances for survival of both the host plant and the pathogen.


2011 ◽  
Vol 57 (3) ◽  
pp. 211-216 ◽  
Author(s):  
Jie Feng ◽  
Feng Wang ◽  
Geoff R. Hughes ◽  
Susan Kaminskyj ◽  
Yangdou Wei

The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is selective for serine esterases. When conidia were inoculated on wheat leaves pretreated with diisopropyl fluorophosphate, both appressorial germ tube differentiation and symptom development were significantly impaired, indicating an important role of secreted serine esterases in wheat powdery mildew disease establishment.


2011 ◽  
Vol 396-398 ◽  
pp. 2012-2017
Author(s):  
Shi Zhou Du ◽  
Wen Jiang Huang ◽  
Rong Fu Wang ◽  
Ju Hua Luo ◽  
Jin Ling Zhao ◽  
...  

The hyperspectral bands sensitive to the disease severity levels of wheat powdery mildew was elucidated in this study. The disease severity levels of wheat powdery mildew were also inverted by the extracting characteristic parameters, which provided a basis for detecting the wheat powdery mildew using hyperspectral data. The spectral data of single leaves was obtained at heading stage, the spectral characteristic parameters and sensitivity of wheat leaves were analyzed qualitatively and quantitatively. The result showed that spectral reflectivity within the visible wavebands (400—760 nm) was increased with the aggravating disease severity levels. The spectral sensitivity reached the maximum value within visible wavebands and the optimal sensitive bands for detecting disease severity levels was 630—680nm. After the spectrum was continuum removal-treated, the absorption position moved to longer wavelength with the aggravating disease severity levels and the disease severity levels showed extremely significant negative correlations with the absorption height, absorption width and absorption area. The linear regression equation has high determination coefficient and low root mean square error using the right AAI as independent variable to establish the model. Moreover, the precision verification test also showed that the model performed best in monitoring wheat powdery mildew. In conclusion, disease severity levels of wheat powdery mildew could be inverted effectively by hyperspectral technology, which provides the foundation for detecting wheat powdery mildew.


2021 ◽  
Author(s):  
Slobodan Vlajic ◽  
◽  
Jelica Gvozdanovic - Varga ◽  
Stevan Masirevic ◽  
Maja Ignjatov ◽  
...  

Powdery mildew of spinach cause significant damage in agriculture production reducing the value usage of the leaves. Considering that there are no registered fungicides for controlling the powdery mildew of spinach in the Republic of Serbia, the aim of the research was to examine various fungicides for preventive and therapeutic usage. The study included seven synthetic and one biological fungicide. The lowest intensity of infection in preventive (0.10%) and therapeutic (0.33%) treatment was found when using a combination of active substances famoxadone + cimoxanil. However, when choosing and applying fungicides in spinach, special attention must be dedicate to the withdrawal period of the formulated products.


2016 ◽  
pp. 73-80
Author(s):  
Xénia Pálfi ◽  
Dénes Bisztray ◽  
Szabolcs Villangó ◽  
Zita Pálfi ◽  
Tamás Deák ◽  
...  

The aim of the present study was to examine the efficiency of paraffin oil against powdery mildew in Eger wine region. The experiment has been carried out in 2013 and 2014 with Chardonnay and Kékfrankos grape varieties, which have different resistance against powdery mildew. The effectiveness of the oil was examined on leaves and clusters (frequency and intensity). This oil was effective against Erysiphe necator infection on field trials in Chile and Brazil. The spread of downy mildew (Plasmopara viticola) was also inhibited by this material in some experiments conducted in Spain and France. The differences between oil treatments represented the sensitivity of the grape varieties in accordance with the applied dosages. The oil was effective against powdery mildew with different extent as a result of the so called ”vintage effect”. In 2013, the treatment of the highest dosage (D3) didn’t differed significantly in frequency and intensity of infection from the regular treatment (clusters of Kékfrankos, leaves of Chardonnay). In 2014, the oil was not so effective against powdery mildew compared to 2013. No remarkable differences were detected between the treatments due to the strong pressure of powdery mildew. Furthermore, no any effect of the lowest dosage (D1) was detected in the case of the sensitive clusters of Chardonnay and leaves of Kékfrankos in both experimental years. In summary, the oil treatment has an effect against powdery mildew, however this efficiency largely depends on the vintage characteristics and the pressure of powdery mildew. Further investigations are neccessary, for example field trials with combinations of other sprays. The oil can be useable as fungicide with proper care in eco-friendly integrated and bio (ecological) viticulture.


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