Modulation of growth and differentiation in normal human keratinocytes by transforming growth factor-β 1

1990 ◽  
Vol 1 (5) ◽  
pp. 394
Author(s):  
Makoto Hashiro ◽  
Hidenobu Okumura ◽  
Kunio Matsumoto ◽  
Koji Hashimoto ◽  
Kunihiko Yoshikawa
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Human Keratinocytes ◽  
Transforming Growth Factor Β ◽  
Normal Human Keratinocytes ◽  
Normal Human

scholarly journals PKCα mediates TGFβ-induced growth inhibition of human keratinocytes via phosphorylation of S100C/A11

2004 ◽  
Vol 164 (7) ◽  
pp. 979-984 ◽  
Author(s):  
Masakiyo Sakaguchi ◽  
Masahiro Miyazaki ◽  
Hiroyuki Sonegawa ◽  
Mariko Kashiwagi ◽  
Motoi Ohba ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Growth Regulation ◽  
Transforming Growth Factor ◽  
Human Keratinocytes ◽  
Transforming Growth Factor Β ◽  
Signal Pathway ◽  
Critical Event ◽  
Kinase C ◽  
Normal Human Keratinocytes ◽  
Normal Human

Growth regulation of epithelial cells is of major concern because most human cancers arise from them. We demonstrated previously a novel signal pathway involving S100C/A11 for high Ca2+-induced growth inhibition of normal human keratinocytes (Sakaguchi, M., M. Miyazaki, M. Takaishi, Y. Sakaguchi, E. Makino, N. Kataoka, H. Yamada, M. Namba, and N.H. Huh. 2003. J. Cell Biol. 163:825–835). This paper addresses a question whether transforming growth factor β (TGFβ) shares the pathway with high Ca2+. On exposure of the cells to TGFβ1, S100C/A11 was phosphorylated, bound to nucleolin, and transferred to the nucleus, resulting in induction of p21WAF1/CIP1 and p15INK4B through activation of Sp1. Protein kinase C α (PKCα) was shown to phosphorylate 10Thr of S100C/A11, which is a critical event for the signal transduction. The TGFβ1-induced growth inhibition was almost completely mitigated when PKCα activity was blocked or when S100C/A11 was functionally sequestered. These results indicate that, in addition to the well-characterized Smad-mediated pathway, the PKCα–S100C/A11-mediated pathway is involved in and essential for the growth inhibition of normal human keratinocytes cells by TGFβ1.

Modulation of growth and differentiation in normal human keratinocytes by transforming growth factor-?

1990 ◽  
Vol 145 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Kunio Matsumoto ◽  
Koji Hashimoto ◽  
Makoto Hashiro ◽  
Hidenobu Yoshimasa ◽  
Kunihiko Yoshikawa
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Normal Human
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