FC17 non-invasive in vivo studies of pharmacokinetics and metabolic parameters using EPR spectroscopy

AbstractBrazilian health care programs recommend the use of cariostatic solutions of silver diamine fluoride (SDF) or sodium fluoride varnishes for children 0 to 3 years old with high or moderate caries activity for the control of caries lesions. SDF is a safe, economical, efficient and non-invasive coadjuvant agent, exerting an antibacterial action capable of reducing superficial mineral loss of the enamel and can be used in the treatment of deciduous and permanent teeth. The objective of this literature review was to gather current information on describing the mechanism of SDF action’ and its clinical application in young children in caries prevention and paralysis. The PubMed / Medline and Cochrane Library databases were accessed by identifying the relevant studies published in English from 1960 to May 2017. The search strategy employed the keywords: “Silver diamine fluoride” and “Children “or “Infant” and “Caries prevention”. Data extraction was performed in: 19 in vitro studies; 10 review articles and 8 in vivo studies. It was possible to conclude that SDF is almost twice as effective compared to fluoride varnish in caries paralysis. However, the contact time of the solution and the optimal frequency of application of the SDF are still undefined, inducing new projects and clinical studies in the search for an adequate clinical protocol of this cariostatic.Keywords: Preventive Dentistry. Children. Dental Caries.Resumo Os programas de assistência à saúde brasileiros recomendam para o controle das lesões de cárie a utilização de soluções cariostáticas de diamino fluoreto de prata - DFP ou vernizes de fluoreto de sódio para crianças de 0 a 3 anos com atividade de cárie alta ou moderada. O DFP é agente coadjuvante seguro, econômico, eficiente e não invasivo, exercendo ação antibacteriana capaz de reduzir a perda mineral superficial do esmalte e, pode ser utilizado no tratamento de dentes decíduos e permanentes. O objetivo desta revisão de literatura foi reunir informações atuais sobre descrever o mecanismo de ação do DFP e sua aplicação clínica em crianças de pouca idade na prevenção e paralisação da cárie. As bases de dados PubMed/Medline e Cochane Library foram acessadas identificando os estudos relevantes publicados em inglês, no período de 1960 a maio de 2017. A estratégia de busca empregou as palavras-chave: “Silver diamine fluoride” and “Children” or “Infant” and “Caries prevention”. A extração dos dados foi realizada em: 19 estudos in vitro; 10 artigos de revisão e 8 estudos in vivo. Foi possível concluir que o DFP apresenta-se quase duas vezes tão eficaz comparado ao verniz fluoretado na paralização de cárie. Porém, o tempo de contato da solução e a frequência ótima de aplicação do DFP ainda estão indefinidos, suscitando novos projetos e estudos clínicos na busca de adequado protocolo clínico deste cariostático.Palavra-chave: Odontologia Preventiva. Criança. Carie Dentária.

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Measurements of clinically significant doses of ionizing radiation using non-invasive in vivo EPR spectroscopy of teeth in situ

Applied Radiation and Isotopes ◽

10.1016/j.apradiso.2004.08.016 ◽

2005 ◽

Vol 62 (2) ◽

pp. 293-299 ◽

Cited By ~ 30

Author(s):

Harold M. Swartz ◽

Akinori Iwasaki ◽

Tadeusz Walczak ◽

Eugene Demidenko ◽

Ildar Salikov ◽

...

Keyword(s):

Ionizing Radiation ◽

Epr Spectroscopy ◽

Non Invasive ◽

Clinically Significant ◽

In Vivo Epr

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NMR spectroscopy of a single mammalian embryo

10.1101/2021.07.26.453772 ◽

2021 ◽

Author(s):

Giulia Sivelli ◽

Gaurasundar Marc Conley ◽

Carolina Herrera ◽

Kathryn Marable ◽

Kyle Rodriguez ◽

...

Keyword(s):

Building Blocks ◽

Resolving Power ◽

In Vivo Studies ◽

Small Samples ◽

Single Chip ◽

Mammalian Embryo ◽

Non Invasive ◽

Spectral Peaks ◽

Mammalian Embryos

The resolving power, chemical sensitivity and non-invasive nature of NMR has made it an established technique for in vivo studies of large organisms both for research and clinical applications. These features would clearly be beneficial at the nanoliter scale (nL), typical of early development of mammalian embryos, microtissues and organoids, the scale where the building blocks of complex organisms could be observed. However, the handling of such small samples (about 100 micrometers) and sensitivity issues have prevented the widespread adoption of NMR. Recently we have shown how these limitations can be overcome with ultra-compact single-chip probes. In this article we show that such probes have sufficient sensitivity to detect and resolve NMR signals from individual bovine pre-implantation embryos. In less than 1 hour these spherical samples of just 130-190 micrometers produce distinct spectral peaks, largely originating from lipids contained inside them. We further observe how the spectral features, namely the peak intensities, vary from one sample to another despite their optical and morphological similarities.

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SU-C-BRD-05: Non-Invasive in Vivo Biodosimetry in Radiotherapy Patients Using Electron Paramagnetic Resonance (EPR) Spectroscopy

Medical Physics ◽

10.1118/1.4923800 ◽

2015 ◽

Vol 42 (6Part2) ◽

pp. 3192-3193 ◽

Cited By ~ 5

Author(s):

N Bahar ◽

K Roberts ◽

F Stabile ◽

N Mongillo ◽

RD Decker ◽

...

Keyword(s):

Electron Paramagnetic Resonance ◽

Epr Spectroscopy ◽

Paramagnetic Resonance ◽

Non Invasive ◽

Electron Paramagnetic

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Non-invasive in vivo evaluation of in situ forming PLGA implants by benchtop magnetic resonance imaging (BT-MRI) and EPR spectroscopy

European Journal of Pharmaceutics and Biopharmaceutics ◽

10.1016/j.ejpb.2009.06.008 ◽

2010 ◽

Vol 74 (1) ◽

pp. 102-108 ◽

Cited By ~ 38

Author(s):

Sabine Kempe ◽

Hendrik Metz ◽

Priscila G.C. Pereira ◽

Karsten Mäder

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Epr Spectroscopy ◽

Resonance Imaging ◽

In Vivo Evaluation ◽

Non Invasive ◽

In Situ Forming

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Superparamagnetic Iron Oxide Nanoparticles (SPIONs) for Diagnosis and Treatment of Breast, Ovarian and Cervical Cancers

Current Drug Metabolism ◽

10.2174/1389200220666191016124958 ◽

2020 ◽

Vol 20 (12) ◽

pp. 942-945 ◽

Cited By ~ 1

Author(s):

Sekhar Talluri ◽

Rama R. Malla

Keyword(s):

Cervical Cancer ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Diagnosis And Treatment ◽

In Vivo Studies ◽

Oxide Nanoparticles ◽

Therapeutic Applications ◽

Non Invasive ◽

Theranostic Agents

Background: The potential of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) as theranostic agents for cancer has been investigated extensively. SPIONS can be utilized for diagnostic imaging, drug delivery as well as for therapeutic applications. SPIONS are of particular interest because of their potential for non-invasive diagnosis and non-invasive therapeutic applications. This article is a review of in vivo and clinical studies of SPIONs for diagnosis and treatment of breast, ovarian and cervical cancer. The current limitations of this technology with relation to clinical therapeutic applications and the potential to overcome these limitations are also discussed. Methods: NCBI Pubmed was searched for relevant documents by using keyword and MESH based search. The following keyword combinations were used: ‘breast cancer’ and SPION, ‘ovarian cancer’ and SPION, and ‘cervical cancer’ and SPION. The resulting list was manually scanned for the studies involving clinical and in vivo studies. Results: The 29 most relevant publications were identified and reviewed. Conclusion: Although numerous in vitro and in vivo studies have demonstrated the safety and effectiveness of the use of SPIONs for both diagnostic and therapeutic applications, there is relatively little progress towards translation to clinical applications involving breast, ovarian and cervical cancer.

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