GONADOTROPIN AND LIPOPROTEIN RECEPTOR LEVELS IN RAT LUTEAL CELLS OF EARLY PREGNANCY

1983 ◽  
pp. 795-803
Author(s):  
WALTER G. WIEST ◽  
YUKIO NAKAMURA ◽  
VIPLA PURI ◽  
D. MICHAEL NELSON
Keyword(s):  
Early Pregnancy ◽  
Lipoprotein Receptor ◽  
Luteal Cells

Gonadotropin and lipoprotein receptor levels in rat luteal cells of early pregnancy

1983 ◽  
Vol 19 (1) ◽  
pp. 795-803 ◽  
Author(s):  
Walter G. Wiest ◽  
Yukio Nakamura ◽  
Vipla Puri ◽  
D.Michael Nelson
Keyword(s):  
Early Pregnancy ◽  
Lipoprotein Receptor ◽  
Luteal Cells

Varied effects of doxorubicin (DOX) on the corpus luteum of C57BL/6 mice during early pregnancy

2021 ◽  
Author(s):  
Christian Lee Andersen ◽  
Haeyeun Byun ◽  
Yuehuan Li ◽  
Shuo Xiao ◽  
Doris M Miller ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Lipid Droplets ◽  
Luteal Cell ◽  
Knowledge Gap ◽  
Chemotherapeutic Drugs ◽  
Luteal Cells ◽  
Single Intraperitoneal Injection ◽  
Premenopausal Patients

Abstract Certain chemotherapeutic drugs are toxic to ovarian follicles. The corpus luteum (CL) is normally developed from an ovulated follicle for producing progesterone (P4) to support early pregnancy. To fill in the knowledge gap about effects of chemotherapy on the CL, we tested the hypothesis that chemotherapy may target endothelial cells and/or luteal cells in the CL to impair CL function in P4 steroidogenesis using doxorubicin (DOX) as a representative chemotherapeutic drug in mice. In both mixed background mice and C57BL/6 mice, a single intraperitoneal injection of DOX (10 mg/kg) on 0.5 days post coitum (D0.5, post-ovulation) led to ~58% D3.5 mice with serum P4 levels lower than the serum P4 range in the PBS-treated control mice. Further studies in the C57BL/6 ovaries revealed that CLs from DOX-treated mice with low P4 levels had less defined luteal cords and disrupted collagen IV expression pattern, indicating disrupted capillary, accompanied with less differentiated luteal cells that had smaller cytoplasm and reduced StAR expression. DOX-treated ovaries had increased granulosa cell death in the growing follicles, reduced PCNA-positive endothelial cells in the CLs, enlarged lipid droplets and disrupted F-actin in the luteal cells. These novel data suggest that the proliferating endothelial cells in the developing CL may be the primary target of DOX to impair the vascular support for luteal cell differentiation and subsequently P4 steroidogenesis. This study fills in the knowledge gap about the toxic effects of chemotherapy on the CL and provides critical information for risk assessment of chemotherapy in premenopausal patients.

The effect of bromocriptine during early pregnancy in the rat: inhibition of implantation

1980 ◽  
Vol 94 (2) ◽  
pp. 268-272 ◽  
Author(s):  
U. Müller ◽  
Th. Bauknecht ◽  
J. W. Siebers
Keyword(s):  
Body Weight ◽  
Early Pregnancy ◽  
Prolactin Secretion ◽  
Teratogenic Effect ◽  
Preimplantation Embryos ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Uterine Mucosa

Abstract. The increase in the number of the ovarian LH/hCG-receptors and of serum progesterone concentration during pregnancy in the rat is highly dependent on the presence of prolactin during the luteinization process. Blocking of the hypophyseal prolactin secretion by twice daily injections of 2 μg/g body weight bromocriptine into rats from the first day of pregnancy onwards prevents the increase in ovarian hCG-binding and progesterone production. Similar results are obtained by application of bromocriptine during the first three days only or either at day 1, 3 or 5 after mating. In all bromocriptine treated rats implantation of blastocysts does not occur. The failure to implant is not due to a teratogenic effect of bromocriptine onto preimplantation embryos, but is probably the consequence of changes of the uterine mucosa caused by the lack of progesterone production in the luteal cells.

Size distribution of bovine steroidogenic luteal cells during pregnancy

2001 ◽  
Vol 73 (2) ◽  
pp. 323-327 ◽  
Author(s):  
Ş Arikan ◽  
A. Yigit
Keyword(s):  
Corpus Luteum ◽  
Size Distribution ◽  
Early Pregnancy ◽  
Wide Spectrum ◽  
Cell Diameter ◽  
Corpora Lutea ◽  
Single Cell Suspension ◽  
Luteal Cells ◽  
Luteal Tissue ◽  
Steroidogenic Cells

AbstractThis study was designed to investigate the size distribution of bovine steroidogenic luteal cells throughout pregnancy. Corpora lutea collected from three different stages of pregnancy were used. Luteal tissue was dissociated into single-cell suspension by enzyme treatments. Cells were stained for 3β-hydroxysteroid dehydrogenase (HSD) activity a marker for steroidogenic cells. The steroidogenic cells covered a wide spectrum of size ranging from 10 to 60 µm in diameter. There was a significant increase in mean cell diameter (P > 0·05) as pregnancy progressed. Mean diameter of 3β-HSD positive cells increased from 17·03 (s.e. 1·3) µm in the corpus luteum of early pregnancy to 33·38 (s.e. 2·4) µm in the corpus luteum of advanced pregnancy. The ratio of large (>22 µm in diameter) to small (10 to 22 µm in diameter) luteal cells was 0·32 : 1·0 in the early pregnancy, with the 10 to 22 µm cell size class predominant. However, the ratio of large to small luteal cells was increased to 6·49 : 1·0 µm as pregnancy advanced and 23 to 42 µm cell sizes become predominant. It is likely that small luteal cells develop into large cells as gestation progresses. Development of pregnancy is associated with an increase in size of steroidogenic luteal cells.

Human large luteal cells in the menstrual cycle and early pregnancy express leukotriene A4 hydrolase

1998 ◽  
Vol 4 (8) ◽  
pp. 803-810 ◽  
Author(s):  
N. Hattori ◽  
H. Fujiwara ◽  
M. Maeda ◽  
S. Yoshioka ◽  
T. Higuchi ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Early Pregnancy ◽  
Luteal Cells ◽  
Leukotriene A4 Hydrolase ◽  
Leukotriene A4

scholarly journals Antioxidant enzymatic defence systems in sheep corpus luteum throughout pregnancy

Reproduction ◽  
2004 ◽  
Vol 128 (6) ◽  
pp. 767-774 ◽  
Author(s):  
Kaïs H Al-Gubory ◽  
Philippe Bolifraud ◽  
Guy Germain ◽  
Annie Nicole ◽  
Irène Ceballos-Bicot
Keyword(s):  
Antioxidant Enzymes ◽  
Enzymatic Activity ◽  
Early Pregnancy ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Copper Zinc Superoxide Dismutase ◽  
Zinc Superoxide Dismutase ◽  
Copper Zinc ◽  
Cellular Integrity

The activities of copper, zinc-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), glutathione reductase (GSSG-R) and glutathioneS-transferase (GST) were studied in sheep corpora lutea (CL) obtained on days 15, 40, 60, 80 and 128 of pregnancy. Maintained enzymatic activity of SOD1, SOD2, GPX, GSSG-R and GST were found in the sheep CL throughout pregnancy. Enzymatic activity of SOD1, GPX and GST increased significantly from day 15 to day 40 of pregnancy, and thereafter remained constant until day 128. SOD2 and GSSG-R activities were not different between any days of pregnancy examined. Apoptotic luteal cells identified by the terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick-end labelling were very rarely observed, and their incidence (less than 0.5%) was not different between days of pregnancy. These results showed that the activities of antioxidant enzymes in the sheep CL are subject to major changes during early pregnancy, suggesting that the CL of early pregnancy may be rescued from luteolysis through increasing activities of key antioxidant enzymes and inhibition of apoptosis. Maintained levels of antioxidant enzymes in the CL throughout pregnancy may be linked to reactive oxygen species continuously generated in the steroidogenically active luteal cells, and may be involved in the maintenance of luteal steroidogenic activity and cellular integrity.

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