REPORT ON THE WORKSHOP: “ANTIBODY-DEPENDENT CYTOTOXIC ACTIVITY AS A PROPERTY OF Fc RECEPTOR CELLS”

1972 ◽

Vol 30 ◽

pp. 60-61 ◽

Cited By ~ 1

Author(s):

Robert F. Dunn

Keyword(s):

Fine Particles ◽

Apical Cell ◽

Apical Region ◽

Receptor Cells ◽

Cuticular Plate ◽

Morphological Organization ◽

Crista Ampullaris ◽

Cytoplasmic Organization ◽

High Degree ◽

General Appearance

Receptor cells of the cristae in the vestibular labyrinth of the bullfrog, Rana catesbiana, show a high degree of morphological organization. Four specialized regions may be distinguished: the apical region, the supranuclear region, the paranuclear region, and the basilar region.The apical region includes a single kinocilium, approximately 40 stereocilia, and many small microvilli all projecting from the apical cell surface into the lumen of the ampulla. A cuticular plate, located at the base of the stereocilia, contains filamentous attachments of the stereocilia, and has the general appearance of a homogeneous aggregation of fine particles (Fig. 1). An accumulation of mitochondria is located within the cytoplasm basal to the cuticular plate.

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Ultrastructural aspects of olfactory signal transduction and its development

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010016844x ◽

1994 ◽

Vol 52 ◽

pp. 142-143

Author(s):

Bert Ph. M. Menco

Keyword(s):

Signal Transduction ◽

Olfactory Receptor ◽

Receptor Cell ◽

Freeze Substitution ◽

Luminal Surface ◽

Tissue Sections ◽

Olfactory Receptor Cells ◽

Receptor Cells ◽

Olfactory Signal ◽

Odor Molecule

Vertebrate olfactory receptor cells are specialized neurons that have numerous long tapering cilia. The distal parts of these cilia line the interface between the external odorous environment and the luminal surface of the olfactory epithelium. The length and number of these cilia results in a large surface area that presumably increases the chance that an odor molecule will meet a receptor cell. Advanced methods of cryoprepration and immuno-gold labeling were particularly useful to preserve the delicate ultrastructural and immunocytochemical features of olfactory cilia required for localization of molecules involved in olfactory signal-transduction. We subjected olfactory tissues to freeze-substitution in acetone (unfixed tissues) or methanol (fixed tissues) followed by low temperature embedding in Lowicryl K11M for that purpose. Tissue sections were immunoreacted with several antibodies against proteins that are presumably important in olfactory signal-transduction.

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Ultrastructural study of cytoskeletal interactions with endosomes in macrophages by quickfreezing and deep-etching method

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160431 ◽

1990 ◽

Vol 48 (3) ◽

pp. 576-577

Author(s):

Takeshi Baba ◽

Nobuki Shiozawa ◽

Masao Hotch ◽

Shinichi Ohno

Keyword(s):

Immune Complex ◽

Ultrastructural Study ◽

Fc Receptor ◽

Coated Pits ◽

Deep Etching ◽

Receptor Mediated Endocytosis ◽

Etching Method ◽

Quick Freezing

Endosomes are vesicular or tubular organelles that play important roles in transports of receptors and receptor―bound ligands during receptor-mediated endocytosis. The mechanisms of endocytic transports from clathrin-coated pits to lysosomes have been studied by many investigators. However, few studies were reported about the interactions between endosomes and cytoskeletons. In this study, Fc-receptor-mediated endocytosis in macrophages are investigated by quick-freezing and deep-etching (QF-DE) method combined with gold-labeled immune complex and “replica scraping method”.

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