IMMUNOLOGICAL INVESTIGATIONS OF HOUSE-DUST AND HOUSE-DUST MITES II. LOCALIZATION OF THE ANTIGEN IN THE BODY OF THE HOUSE-DUST MITE DERMATOPHAGOIDES PTERONYSSINUS BY MEANS OF THE INDIRECT IMMUNO-FLUORESCENCE METHOD

1979 ◽  
pp. 205-210 ◽  
Author(s):  
Y. Mumcuoglu ◽  
Th. Rufli
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Dermatophagoides Pteronyssinus ◽  
The Body ◽  
Fluorescence Method ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dust Mite

scholarly journals JENIS DAN KEPADATAN TUNGAU DEBU RUMAH PADA BEBERAPA HABITAT DI RUMAH PENDERITA PENYAKIT ALERGI

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Wiska F. Ponggalunggu ◽  
Victor D. Pijoh ◽  
Greta J. P. Wahongan
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Allergic Diseases ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Cross Sectional ◽  
Dust Mite ◽  
Euroglyphus Maynei

Abstract. House dust mites are the most found allergens in homes scattered throughout the world that associated with allergic manifestations in the respiratory tract and skin, such as bronchial asthma, allergic rhinitis and atopic dermatitis. The most common house dust mite is from the family Pyroglyphidae, four of which have a close relationship with the incidence of allergy: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, and Euroglyphus maynei. This study aims to determine the type and density of house dust mites in several habitats in the homes of people with allergic diseases. This study is a descriptive study using cross-sectional method. Samples were dust in allergic patient’s home in Malalayang I, taken from three habitats, namely bed (mattress and bed linen), bedrooms floor and sofa, as many as 96 samples. Of the 96 samples, there are 60 samples positive of dust mites and 36 negative samples. This study found 157 mites (124 adults and 33 larvae mites). Of the 124 adult mites were identified, there are 122 from order Astigmata with the dominant species is Dermatophagoides pteronyssinus, and two others of the order Prostigmata. In each habitat that is in the bed (mattress and bed linen), bedrooms floor and sofa, Pyroglyphidae is the most prevalent.Keywords: house dust mite, allergiesAbstrak. Tungau debu rumah (TDR) adalah alergen dalam rumah terbanyak yang tersebar di seluruh dunia yang dikaitkan dengan manifestasi alergi pada saluran pernapasan dan kulit, seperti asma bronkial, rinitis alergi dan dermatitis atopik. TDR yang paling sering ditemukan adalah dari famili Pyroglyphidae, empat di antaranya memiliki hubungan yang erat dengan kejadian alergi: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, dan Euroglyphus maynei. Penelitian ini bertujuan untuk mengetahui jenis dan kepadatan tungau debu rumah pada beberapa habitat di rumah penderita penyakit alergi. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode cross-sectional. Sampel penelitian adalah debu di rumah penderita alergi di Kelurahan Malalayang I yang diambil dari 3 habitat, yaitu tempat tidur (kasur dan sprei), lantai kamar tidur, dan sofa sebanyak 96 sampel. Dari 96 sampel, 60 sampel positif tungau debu dan 36 sampel negatif. Pada penelitian ini ditemukan 157 tungau (124 tungau dewasa dan 33 larva). Dari 124 tungau dewasa yang terindentifikasi, dari ordo Astigmata sebanyak 122 dengan spesies yang dominan adalah Dermatophagoides pteronyssinus, dan dua lainnya dari ordo Prostigmata. Pada masing-masing habitat yaitu di tempat tidur (kasur dan sprei), lantai kamar tidur dan sofa, famili Pyroglyphidae paling banyak ditemukan.Kata kunci: tungau debu rumah, alergi

scholarly journals PARTICULAR QUALITIES OF THE TREATMENT IN PRE-SCHOOL AGE CHILDREN SENSITIZED TO HOUSE DUST MITES ALLERGEN

2017 ◽  
Vol 4 (2) ◽  
pp. 72-78
Author(s):  
Olena Sharikadze
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
House Dust Mite ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Atopic Asthma ◽  
Dust Mites ◽  
Allergen Specific Immunotherapy ◽  
Dust Mite

Sharikadze O.V.Background. This study determined the efficacy of sublingual allergen–specific immunotherapy (SLIT) in Ukrainian children younger than 5 years of age with allergic rhinitis and bronchial asthma sensitized to house dust mite allergens. Methods. A cohort prospective study was conducted over a two years period with assessment of the sensitization towards inhalant allergens, measured in-vivo by wheal size and number of positive reactions on the standard skin prick test (SPT). SPSS was used to analyse any statistical correlation. Results. A total of 125 atopic asthma or/and allergic rhinitis with the mean age of 4.11 ±0.83 who were sensitized to house dust mites Dermatophagoides pteronyssinus and/or Dermatophagoides farina were included. 47 children received two-years SLIT to treat house dust mite allergies using standardized extract of sublingual allergens containing a mixture of house dust mites (Der.  pteronyssinus and Der. farinae) in the correlation of 1(0,175 НЕР):1(0,175 НЕР) (Diater, Spain). These patients were mono-sensitized with Der. pteronyssinus and Der. farinae or were SPT poly-sensitized, but these patients only had house dust mite allergy symptoms. In this group the assessment of clinical efficacy of SLIT showed the significant differences in VAS before treatment and 6 months after its receiving. The symptom “nasal obstruction” was assessed as 2.3±-1.6 points  at the beginning of the therapy, and the data reduced almost twice (1.3±-1.1); Р <0,05 after 6  months of SLIT;  then (12 and 24 months) it decreased to 0.91±0.9 and 0.34±0.5; Р <0,01, correspondingly. Conclusion. This study has shown that using of SLIT in atopic asthma or/and allergic rhinitis children under 5 year sensitized to house dust mites is an effective and safe treatment method and allows to quickly (over the first 6 months of SLIT) control the symptoms. The data of comparative analysis in the group of patients who didn’t receive SLIT has pointed to high frequency of disease symptom occurrence after the end of baseline therapy.KeyWords: sublingual allergen-specific immunotherapy, children, bronchial asthma, allergic rhinitis, sensitization, allergens, Diater  ОСОБЛИВОСТІ ЛІКУВАННЯ ДІТЕЙ ДОШКІЛЬНОГО ВІКУ, СЕНСІБІЛІЗОВАНИХ ДО АЛЕРГЕНІВ ДОМАШНЬОГО ПИЛУШарикадзе О.В.Це дослідження визначило ефективність сублингвально алерген-специфічної імунотерапії (SLIT) українських дітей молодше 5 років з алергічним ринітом та бронхіальною астмою, сенсибілізованих до алергенів пилових кліщів. Методи. Проспективне дослідження когорти проводилося протягом двох років з оцінкою сенсибілізації до інгаляційних алергенів, виміряної in-vivo за розміром пухирів і кількості позитивних реакцій на стандартному тесті на шкірі (SPT). Для аналізу статистики використовувався пакет SPSS. Результати. Обстежено 125 дітей (середній вік 4,11 ± 0,83 років) з атопічною астмою та/або алергічним ринітом, які були сенсибілізовані до домашніх пилових кліщів Dermatophagoides pteronyssinus та/або Dermatophagoides farina. 47 дітей отримали дворічний курс SLIT з використанням стандартизованого екстракту сублінгвальних алергенів, що містять суміш кліщів домашнього пилу (Der. Pteronyssinus і Der. Farinae) в співвідношенні 1 (0,175 НЕР): 1 (0,175 НЕР) (Діатер, Іспанія). Пацієнти були моносенсібіровани Dermatophagoides pteronyssinus і / або Dermatophagoides farina або були полісенсібілізовані (за SPT), однак симптоми розвивалися тільки на домашній пил. У цій групі оцінка клінічної ефективності SLIT показала значні відмінності до лікування і через 6 місяців після його отримання. Симптом «носової обструкції» оцінювався як 2,3 ± 1,6 бала на початку терапії і зменшувалися майже в два рази (1,3 ± 1,1); Р <0,05 після 6 місяців SLIT. Потім (12 і 24 місяці) він знизився до 0,91 ± 0,9 і 0,34 ± 0,5; Р <0,01, відповідно. Висновок. Дослідження показало, що використання SLIT при атопічній астмі та/або алергічному риніті у дітей до 5 років, сенсибілізованих до домашніх пилових кліщів, є ефективним і безпечним методом лікування і дозволяє швидко (протягом перших 6 місяців SLIT) контролювати симптоми. Дані порівняльного аналізу в групі пацієнтів, які не отримували SLIT, вказують на високу частоту виникнення симптомів хвороби після закінчення базової терапії.Ключові слова: сублінгвальна алерген-специфічна імунотерапія, діти, бронхіальна астма, алергічний риніт, сенсибілізація, алергени, Діатер. ОСОБЕННОСТИ ЛЕЧЕНИЯ ДЕТЕЙ ДОШКОЛЬНОГО ВОЗРАСТА, СЕНСИБИЛИЗИРОВАННЫХ АЛЛЕРГЕНАМИ ДОМАШНЕЙ ПЫЛИШарикадзе Е.В.Это исследование определило эффективность сублингвальной аллерген-специфической иммунотерапии (SLIT) украинских детей моложе 5 лет с аллергическим ринитом и бронхиальной астмой, сенсибилизированных к аллергенам пылевых клещей. Методы. Проспективное исследование когорты проводилось в течение двух лет с оценкой сенсибилизации к ингаляционным аллергенам, измеренной in-vivo по размеру волдырей и количеству положительных реакций на стандартном тесте на коже (SPT). Для анализа статистики использовался пакет SPSS. Результаты. Обследованы 125 детей (средний возраст 4,11 ± 0,83 лет) с атопической астмой и/или аллергическим ринитом, которые были сенсибилизированы к домашним пылевым клещам Dermatophagoides pteronyssinus и/или Dermatophagoides farina. 47 детей получили двухлетний курс SLIT с использованием стандартизованного экстракта сублингвальных аллергенов, содержащих смесь клещей домашней пыли (Der. Pteronyssinus и Der. Farinae) в соотношении 1 (0,175 НЕР): 1 (0,175 НЕР) (Диатер, Испания). Пациенты были моносенсибированы Dermatophagoides pteronyssinus и/или Dermatophagoides farina или были полисенсибилизированы (по SPT), однако симптомы развивались только на домашнюю пыль. В этой группе оценка клинической эффективности SLIT показала значительные различия до лечения и через 6 месяцев после его получения. Симптом «носовой обструкции» оценивался как 2,3 ± -1,6 балла в начале терапии и уменьшались почти в два раза (1,3 ± -1,1); Р <0,05 после 6 месяцев SLIT. Затем (12 и 24 месяца) он снизился до 0,91 ± 0,9 и 0,34 ± 0,5; Р <0,01, соответственно. Вывод. Исследование показало, что использование SLIT при атопической астме или/или аллергическом рините детей до 5 лет, сенсибилизированных к домашним пылевым клещам, является эффективным и безопасным методом лечения и позволяет быстро (в течение первых 6 месяцев SLIT) контролировать симптомы. Данные сравнительного анализа в группе пациентов, которые не получали SLIT, указывают на высокую частоту появления симптомов болезни после окончания базовой терапии.Ключевые слова: сублингвальная аллерген-специфическая иммунотерапия, дети, бронхиальная астма, аллергический ринит, сенсибилизация, аллергены, Диатер.

scholarly journals Effect of Activated Charcoal Fibers on the Survival of the House Dust Mite, Dermatophagoides pteronyssinus: A Pilot Study

ISRN Allergy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-3
Author(s):  
Hae-Seon Nam ◽  
Sun-Hwa Lee ◽  
Young-Jin Choi ◽  
Joon-Soo Park ◽  
Moon-Kyun Cho ◽  
...  
Keyword(s):  
Home Environment ◽  
House Dust ◽  
House Dust Mite ◽  
Activated Charcoal ◽  
Indoor Environment ◽  
Room Temperature ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dust Mite

House dust mites produce potent allergens that exacerbate asthma in sensitized patients, whom are recommended to practice allergen avoidance within their home environment. We tested the effect of activated charcoal impregnated fibers on house dust mite survival. One hundred live adult house dust mites (Dermatophagoides pteronyssinus) were added to eight culture dishes preequilibrated at room temperature () and 70% humidity () containing house dust mite food and active charcoal fibers. At 10 minute intervals, live and dead house dust mites were counted. All house dust mites instantly attached to the activated charcoal fibers and started to shrink almost immediately. There were no live house dust mites present as early as 40 minutes in some dishes while after 190 minutes all house dust mites were dead. In conclusion, activated charcoal fibers, if incorporated into bedding items, have the potential to control house dust mites in the indoor environment.

scholarly journals Effectiveness of house dust mite acaricide tri-n-butyl tin maleate on carpets, fabrics and mattress foam: a standardization of methodology

2006 ◽  
Vol 48 (3) ◽  
pp. 171-174 ◽  
Author(s):  
Satiko Uehara ◽  
Marcia Regina Franzolin ◽  
Soledad Chiesa ◽  
Débora Moreira ◽  
Walderez Gambale ◽  
...  
Keyword(s):  
Relative Humidity ◽  
House Dust ◽  
House Dust Mite ◽  
Culture Medium ◽  
Petri Dish ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Optimal Conditions ◽  
Dust Mite

The aim of this study was to determine the effectiveness of the acaricide tri-n-butyl tin maleate, industrially applied to samples of carpets, mattress foam, and fabrics used for furniture upholstery, soft toys and shoe uppers. Approximately 100 adult house dust mites of the species Dermatophagoides pteronyssinus were inoculated into a Petri dish containing the sample (a piece of carpet, mattress foam, or fabric), treated with the acaricide, randomly collected. Mite-maintenance culture medium was added on top of each sample. After one, two, three, seven and 30 days of incubation at 25 ºC and 75% relative humidity, each dish was examined using a 40X stereoscopic microscope (40X). One hundred percent acaricide effectiveness was obtained in treated materials by the end of the 30th-day postinoculation period, under optimal conditions for mite maintenance.

scholarly journals House Dust Mites in One of Indonesian Detention Center in 2020

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 98-103
Author(s):  
Ajrina Malia ◽  
Makhabbah Jamilatun ◽  
Aminah Aminah
Keyword(s):  
House Dust ◽  
Descriptive Study ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Detention Center ◽  
Home Furnishings ◽  
Dust Mite ◽  
Flotation Method

House dust mite was one of the most common allergens in homes around the world and associated with manifestations of allergies of skin and respiratory tract such as bronchial asthma, allergic rhinitis, and atopic dermatitis. This arthropod was commonly found on carpets, mattresses, children's toys, and other home furnishings. This study was aimed to identify and quantify the number of Dermatophagoides spp in Detention Center. This descriptive study was conducted using flotation method on 52 samples of mattress dust collected in February 2020. The results showed that 49 out of 52 mattress dust samples were positive of Dermatophagoidesspp..Of all Dermatophagoides spp, there were Dermatophagoides pteronyssinus (71.2%) and Dermatophagoides farinae (28.8%). In conclusion, this study found two species of Dermatophagoides spp. in Detention Center and suggested that the fascility maintain the cleanliness of the mattress by drying the mattress and changing the sheets at least once a week.

Quantitation of house dust mites and house dust mite allergens in the microenvironment of dogs

2003 ◽  
Vol 64 (10) ◽  
pp. 1301-1309 ◽  
Author(s):  
Amy Randall ◽  
Andrew Hillier ◽  
Lynette K. Cole ◽  
Kenneth W. Kwochka ◽  
Glen Needham ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dust Mite

scholarly journals Investigation on risk factors of adult allergic rhinitis in China

2021 ◽  
Author(s):  
Guo Hong ◽  
Zhou Yibo ◽  
Li Fengxia ◽  
Liu Ming Ping ◽  
Cao Yong
Keyword(s):  
Risk Factors ◽  
Allergic Rhinitis ◽  
House Dust ◽  
House Dust Mite ◽  
Air Pressure ◽  
House Dust Mites ◽  
Control Group ◽  
Observation Group ◽  
Dust Mites ◽  
Dust Mite

Abstract OBJECTIVE: To investigate the risk factors associated with allergic rhinitis in adults. METHODS: Using the 1:1 matching principle, 1096 cases of allergic rhinitis patients aged 18-65 years were selected as the observation group, and another group of healthy medical checkups were selected as the control group, and the data related to allergic rhinitis were collected by allergen testing and questionnaire survey, and univariate and multifactorial analyses were performed respectively. RESULTS: The univariate analysis showed that allergic rhinitis may be related to temperature changes, dryness and humidity, air pressure, wind speed, seasonal changes and exposure to allergens such as dust mites and house dust mites. After allergen testing of all patients in the observation group, it was found that dust mite and house dust mite were the highest percentage of allergens, accounting for 40.51%, indicating that environmental factors play a decisive role in allergic rhinitis. Multi-factor analysis showed that exposure to dust mites, house dust mites, Penicillium punctatum, Streptomyces crossatus and Aspergillus fumigatus were independent risk factors for allergic rhinitis when it was humid, when it was cold, when it was windy and when the air pressure was low. CONCLUSION: The epidemiological status of allergic rhinitis in adults in China was initially understood, and the main allergens were house dust mite and dust mite, which provided scientific epidemiological information for the standardized prevention and treatment of allergic rhinitis in this region.

scholarly journals Do Other Components of Bedding Dust Affect Sensitisation to House Dust Mites?

ISRN Allergy ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-4
Author(s):  
Claire Smith ◽  
Thorsten Stanley ◽  
Julian Crane ◽  
Robert Siebers
Keyword(s):  
Patch Test ◽  
House Dust ◽  
House Dust Mite ◽  
House Dust Mites ◽  
Dust Mites ◽  
Prick Test ◽  
Significant Difference ◽  
Dust Mite ◽  
Der P 1 ◽  
Asymptomatic Subjects

Bedding dust is a mixture of many components, of which the house dust mite (HDM) allergen, Der p 1, is the most allergenic. There has been little work to investigate the effect of other bedding dust components on HDM sensitisation. The objective of the study was to determine the effect of endotoxin in bedding dust on the allergic response in HDM-sensitised individuals. Twenty-nine house dust mite-sensitised adults were skin prick and allergen patch tested against a sterile solution of their own bedding dust and against a solution containing the same concentration of Der p 1 as the bedding solution for comparison. There was no significant difference in wheal size between the diluted house dust mite solution and the bedding dust in spite of their high levels of endotoxin. Symptomatic subjects had larger, but not statistically significant, responses to commercial house dust mite solution than asymptomatic subjects. Allergen patch test responses were negative in 22/29 of subjects using either bedding dust solutions or comparable diluted house dust mite solutions. An individual's own bedding dust does not appear to contain factors that enhance skin prick test or atopy patch test responses to house dust mites.

HOUSE DUST MITE ALLERGENS: FROM NATIVE EXTRACTS TO MODIFIED PREPARATIONS

2021 ◽  
Author(s):  
Anna Vasilyeva ◽  
Valentina M. Berzhets ◽  
Nina S. Petrova ◽  
Svetlana V. Khlgatian ◽  
Stanislava Yu. Petrova ◽  
...  
Keyword(s):  
House Dust ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Binding Reaction ◽  
Dust Mite ◽  
Allergenic Activity ◽  
Elisa Inhibition

The growth of allergic diseases dictates the need to develop new forms of therapeutic allergens with high immunogenic and low allergenic activity. For many years, our laboratory has been developing drugs for the diagnosis and treatment of house dust mites (HDM) allergies. The purpose of this study is to summarize the results of the five-year development of therapeutic preparations of HDM allergens. During this period, we obtained the following forms of therapeutic allergens: a granular sublingual dosage form of a mixed allergen from Dermatophagoides pteronyssinus (Der.p) and Dermatophagoides farinaе (Der.f) and a succinylated monomeric HDM allergoid Der.p. The physicochemical and immunobiological properties of the obtained preparations were studied by methods: electrophoresis in PAGE in the presence of SDS-sodium, micropoint immunoblot, ELISA, inhibition of the binding reaction of allergen-specific IgE in the sera of patients. The research results showed that the obtained preparations have a reduced allergenic and increased immunogenic activity in comparison with native extracts. The created forms of mite allergens can be further used to treat patients sensitized to HDM of the genus Dermatophagoides.

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