Bioproduction of volatile fatty acids from vegetable waste

Author(s):  
Zhao Youcai ◽  
Wei Ran
Author(s):  
Maria Cristina Rizk ◽  
Rosangela Bergamasco ◽  
Célia Regina Granhen Tavares

Fruit and vegetable wastes are generated in large quantities around the world. This kind of residue constitutes a source of nuisance in municipal landfills because of its high biodegradability. Another residue that is generated in large quantities around the world and also constitutes a source of nuisance is the sewage sludge. Both residues can be treated together by the anaerobic co-digestion process. In this sense, the aim of the present study was to evaluate the anaerobic co-digestion of fruit and vegetable waste and sewage sludge, in order to monitor the waste stabilization time and the biogas generation, among others. The study was conducted in a 70 litter stainless steel anaerobic reactor, with no mixing system, at room temperature (25±5ºC), during a period of 105 days. The fruit and vegetable wastes were collected in a central distribution market for food, and were shredded and blended before the experiment. The seed inoculum was collected in an anaerobic domestic sewage station of treatment. The parameters analyzed were: chemical oxygen demand, C/N and C/N/P ratios, pH, alkalinity, volatile fatty acids, and biogas generation. The results showed that the chemical oxygen demand was reduced around 20%. The parameters, pH, alkalinity and volatile fatty acids were stabilized, but the C/N wasn’t stabilized. The final value obtained for C/N ratio was around 20/1. The biogas generation was around 331 litters and most of the biogas production occurred during the first month of the experiment. The low chemical oxygen demand removal, the high C/N ratio and the biogas generation almost always in the first few days of the experiment probably indicate that this residue, although being organic, presents difficulties in its degradation, maybe because of the high organic load that was applied to the reactor. The fact of the reactor being disproved by a mixing system can also have contributed to the low residue degradation.


RSC Advances ◽  
2020 ◽  
Vol 10 (55) ◽  
pp. 33261-33267
Author(s):  
Tao Xing ◽  
Shutai Yu ◽  
Feng Zhen ◽  
Xiaoying Kong ◽  
Yongming Sun

pH and co-digestion conditions were applied for optimal VFA production from hybrid Pennisetum (HP) and fruit and vegetable waste (FVW).


2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.


МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY


1962 ◽  
Vol 21 (1) ◽  
pp. 37-40 ◽  
Author(s):  
H. W. Essig ◽  
U. S. Garrigus ◽  
B. Connor Johnson

1997 ◽  
Vol 34 (5) ◽  
pp. 349-351
Author(s):  
Hiroshi KAMISOYAMA ◽  
Zeng-Tao SUN ◽  
Mineo HASHIGUCHI ◽  
Yutaka ISSHIKI

2021 ◽  
Vol 9 (2) ◽  
pp. 105055
Author(s):  
Yasmim Arantes da Fonseca ◽  
Nayara Clarisse Soares Silva ◽  
Adonai Bruneli de Camargos ◽  
Silvana de Queiroz Silva ◽  
Hector Javier Luna Wandurraga ◽  
...  

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 108
Author(s):  
Yichong Wang ◽  
Sijiong Yu ◽  
Yang Li ◽  
Shuang Zhang ◽  
Xiaolong Qi ◽  
...  

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.


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