Recent trends in characterization of endophytic microorganisms

2022 ◽  
pp. 31-53
Author(s):  
Ayesha T. Tahir ◽  
Jun Kang ◽  
Musfirah Bint-e-Mansoor ◽  
Javeria Ayub ◽  
Zakira Naureen ◽  
...  
2014 ◽  
Vol 30 (2) ◽  
pp. 895-904 ◽  
Author(s):  
K. Venkata Madhuri ◽  
K. Vidya Prabhakar

2015 ◽  
Vol 2015 ◽  
pp. 1-22 ◽  
Author(s):  
Edel Sah ◽  
Hongkee Sah

In recent years, there have been a plethora of nanoengineering approaches for the development of poly(lactide-co-glycolide) (PLGA) nanoparticulate carrier systems. However, overlooking the multifaceted issues in the preparation and characterization of PLGA-based nanoparticles, many reports have been focused on theirin vivobehaviors. It is imperative to fully assess technological aspects of a nanoencapsulation method of choice and to carefully evaluate the nanoparticle quality. The selection of a nanoencapsulation technique should consider drug property, nanoparticle quality, scale-up feasibility, manufacturing costs, personnel safety, environmental impact, waste disposal, and the like. Made in this review are the fundamentals of classical emulsion-templated nanoencapsulation methods used to prepare PLGA nanoparticles. More specifically, this review provides insight into emulsion solvent evaporation/extraction, salting-out, nanoprecipitation, membrane emulsification, microfluidic technology, and flow focusing. Innovative nanoencapsulation techniques are being developed to address many challenges existing in the production of PLGA-based nanoparticles. In addition, there are various out-of-the-box approaches for the development of novel PLGA hybrid systems that could deliver multiple drugs. Latest trends in these areas are also dealt with in this review. Relevant information might be helpful to those who prepare and develop PLGA-based nanoparticles that meet their specific demands.


2003 ◽  
Vol 67 (4) ◽  
pp. 491-502 ◽  
Author(s):  
Gary Strobel ◽  
Bryn Daisy

SUMMARY Endophytic microorganisms are to be found in virtually every plant on earth. These organisms reside in the living tissues of the host plant and do so in a variety of relationships, ranging from symbiotic to slightly pathogenic. Because of what appears to be their contribution to the host plant, the endophytes may produce a plethora of substances of potential use to modern medicine, agriculture, and industry. Novel antibiotics, antimycotics, immunosuppressants, and anticancer compounds are only a few examples of what has been found after the isolation, culture, purification, and characterization of some choice endophytes in the recent past. The potential prospects of finding new drugs that may be effective candidates for treating newly developing diseases in humans, plants, and animals are great.


2020 ◽  
Vol 8 ◽  
Author(s):  
Stefan G. Stanciu ◽  
Loredana Latterini ◽  
Costas A. Charitidis

1997 ◽  
Vol 3 (S2) ◽  
pp. 1245-1246
Author(s):  
F M. Bums ◽  
D. M. Giolando ◽  
J. R. Kirchhoff

Recent trends directed toward the miniaturization of electrodes have produced ultramicroelectrode (UME) devices. Interest in UMEs is due to the considerable improvement in the quality of the electrochemical information that is obtained as the dimensions of the electroactive surface decreases. UMEs exhibit increased sensitivity to analytes, decreased response time, and enhanced single-to-noise. In addition, solution resistance has a decreased influence on the signal. Finally, the reduced size of UMEs allows electroanalysis in small sampling environments. As a result, UMEs have been used in applications for intracellular electroanalysis, as detectors in capillary separation methods and gas chromatography, and as electrochemical probes in scanning electrochemical microscopy.While UMEs have been investigated since the early 1980s, single probe dual UMEs are a recent development. Dual UMEs have two independent UMEs in close proximity, which allows much flexibility in the design of electrochemical-based sensor devices.


2021 ◽  
Vol 8 ◽  
Author(s):  
Yang Woo Kwon ◽  
Han-Seul Jo ◽  
Sungwon Bae ◽  
Youngsuk Seo ◽  
Parkyong Song ◽  
...  

Proteomics has become an important field in molecular sciences, as it provides valuable information on the identity, expression levels, and modification of proteins. For example, cancer proteomics unraveled key information in mechanistic studies on tumor growth and metastasis, which has contributed to the identification of clinically applicable biomarkers as well as therapeutic targets. Several cancer proteome databases have been established and are being shared worldwide. Importantly, the integration of proteomics studies with other omics is providing extensive data related to molecular mechanisms and target modulators. These data may be analyzed and processed through bioinformatic pipelines to obtain useful information. The purpose of this review is to provide an overview of cancer proteomics and recent advances in proteomic techniques. In particular, we aim to offer insights into current proteomics studies of brain cancer, in which proteomic applications are in a relatively early stage. This review covers applications of proteomics from the discovery of biomarkers to the characterization of molecular mechanisms through advances in technology. Moreover, it addresses global trends in proteomics approaches for translational research. As a core method in translational research, the continued development of this field is expected to provide valuable information at a scale beyond that previously seen.


Horticulturae ◽  
2018 ◽  
Vol 4 (4) ◽  
pp. 50 ◽  
Author(s):  
Ortal Galsurker ◽  
Sonia Diskin ◽  
Dalia Maurer ◽  
Oleg Feygenberg ◽  
Noam Alkan

After harvest, the fruit ripens and stem-end rot (SER) starts to develop, leading to significant fruit losses. SER is caused by diverse pathogenic fungi that endophytically colonize the stem during fruit development in the orchard or field and remain quiescent until the onset of fruit ripening. During the endophytic-like stage, the pathogenic fungus colonizes the phloem and xylem of the fruit stem-end; after fruit ripening, the fungus converts to a necrotrophic lifestyle, while colonizing the fruit parenchyma, and causes SER. The fruit stem-end is colonized not only by pathogenic fungi, but also by various nonpathogenic endophytic microorganisms, including fungi, yeast and bacteria. However, little is known about the fruit stem-end endophytic microbiome, which could contain new and existing biocontrol agents. To control fruit SER, treatments such as ripening inhibition, harvesting with the stem, application of chemical or biological fungicides, or physical control such as heat treatments, cold storage, or exposure to light have been suggested. This review focuses on the characterization of SER pathogens, the stem-end microbiome, and different pre- and postharvest practices that could control fruit SER.


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