Smear preparation and simple staining techniques

1978 ◽

Vol 36 (2) ◽

pp. 170-171

Author(s):

Mitsuo Ohtsuki ◽

Michael Sogard

Keyword(s):

Electron Microscope ◽

Phase Contrast ◽

Image Interpretation ◽

Density Lipoprotein ◽

Biological Macromolecules ◽

Contrast Effects ◽

Biological Structure ◽

Electron Microscopic ◽

Structural Investigations ◽

Staining Techniques

Structural investigations of biological macromolecules commonly employ CTEM with negative staining techniques. Difficulties in valid image interpretation arise, however, due to problems such as variability in thickness and degree of penetration of the staining agent, noise from the supporting film, and artifacts from defocus phase contrast effects. In order to determine the effects of these variables on biological structure, as seen by the electron microscope, negative stained macromolecules of high density lipoprotein-3 (HDL3) from human serum were analyzed with both CTEM and STEM, and results were then compared with CTEM micrographs of freeze-etched HDL3. In addition, we altered the structure of this molecule by digesting away its phospholipid component with phospholipase A2 and look for consistent changes in structure.

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A Simple Dichromatic Stain for Plastic Embedded Tissues

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068473 ◽

1970 ◽

Vol 28 ◽

pp. 296-297 ◽

Cited By ~ 1

Author(s):

G. R. Mackay ◽

M. L. Mead

Keyword(s):

Electron Microscopy ◽

Toluidine Blue ◽

Biological Material ◽

Good Reproducibility ◽

Routine Work ◽

Staining Techniques

Color contrasting of 1 to 2 micron sections of plastic embedded biological material is an important adjunct to electron microscopy. The procedures in general use today are simple and rapid giving monochromatic results, e.g., toluidine blue. Although many di- and polychromatic histologic staining techniques have been modified to obtain a counterstaining effect with plasticembedded tissue, the methods are usually undesirable for routine work because they are time consuming, complicated and often defy good reproducibility.

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Effect of ADH on Rat Renal Papilla, an Ultrastructural and Cytochemical Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072496 ◽

1973 ◽

Vol 31 ◽

pp. 410-411

Author(s):

C. N. Sun ◽

H. J. White ◽

E. J. Towbin

Keyword(s):

Electron Microscopy ◽

Diabetes Insipidus ◽

Renal Papilla ◽

Milk Diet ◽

Intercellular Spaces ◽

Cytochemical Study ◽

Collecting Tubule ◽

Concentrate Urine ◽

Staining Techniques ◽

Collecting Tubules

Diabetes insipidus and compulsive water drinking are representative of two categories of antidiuretic hormone (ADH) lack. We studied a strain of rats with congenital diabetes insipidus homozygote (DI) and normal rats on an isocaloric fortified dilute milk diet. In both cases, the collecting tubules could not concentrate urine. Special staining techniques, Alcian Blue-PAS for light microscopy and lanthanum nitrate for electron microscopy were used to demonstrate the changes in interstitial mucopolysaccharides (MPS). The lanthanum staining was done according to the method of Khan and Overton.Electron microscopy shows cytoplasmic lesions, vacules, swelling and degenerating mitochondria and intercellular spaces (IS) in the collecting tubule cells in DI and rats on milk diet.

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Polymer Morphology Revealed by Staining Techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100098204 ◽

1981 ◽

Vol 39 ◽

pp. 340-341

Author(s):

A. C. Reimschuessel ◽

V. Kramer

Keyword(s):

Polymer Melt ◽

Nylon 6 ◽

Morphological Features ◽

Negative Staining ◽

Polymer Morphology ◽

Crystallizable Polymer ◽

Staining Techniques ◽

Long Chain

Staining techniques can be used for either the identification of different polymers or for the differentiation of specific morphological domains within a given polymer. To reveal morphological features in nylon 6, we choose a technique based upon diffusion of the staining agent into accessible regions of the polymer.When a crystallizable polymer - such as nylon 6 - is cooled from the melt, lamellae form by chainfolding of the crystallizing long chain macromolecules. The regions between adjacent lamellae represent the less ordered amorphous domains into which stain can diffuse. In this process the lamellae will be “outlined” by the dense stain, giving rise to contrast comparable to that obtained by “negative” staining techniques.If the cooling of the polymer melt proceeds relatively slowly - as in molding operations - the lamellae are usually arranged in a radial manner. This morphology is referred to as spherulitic.

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The state and spatial distribution of extractives during birch kraft pulping, as evaluated by staining techniques

Nordic Pulp & Paper Research Journal ◽

10.3183/npprj-2005-20-04-p383-391 ◽

2005 ◽

Vol 20 (4) ◽

pp. 383-391 ◽

Cited By ~ 4

Author(s):

Dinesh Fernando ◽

Geoffrey Daniel ◽

Jan Lidén

Keyword(s):

Spatial Distribution ◽

Kraft Pulping ◽

The State ◽

Staining Techniques

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Lugol Iodine Staining Test in Early Detection of Head and Neck Cancer

Revista de Chimie ◽

10.37358/rc.17.8.5797 ◽

2017 ◽

Vol 68 (8) ◽

pp. 1944-1945

Author(s):

Catalin Stefan ◽

Gabriel Lostun ◽

Alexandra Lostun

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Term Outcome ◽

Long Term Outcome ◽

Iodine Staining ◽

Examination Techniques ◽

Staining Techniques ◽

Malignant Lesions

Head and neck cancer represents 3% of malignancies, and it is associated with high mortality due to advanced stage diagnosis. In early stages, the symptomatology can either be absent or be very common, misleading the patient who often ignores it. Early diagnosis of head and neck neoplasia is essential for a favorable long-term outcome. Lately new in vivo examination techniques were developed, and older ones have been improved. Today, in vivo staining techniques are an important tool in the diagnostic of head and neck cancer. Lugol iodine staining method provides valuable information concerning the tumor, allowing the surgeon to differentiate premalignant and malignant lesions.

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Sympathetic input to ganglia of the guinea pig sphincter of Oddi

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1994.266.6.g1162 ◽

1994 ◽

Vol 266 (6) ◽

pp. G1162-G1169 ◽

Cited By ~ 2

Author(s):

D. G. Wells ◽

G. M. Mawe

Keyword(s):

Sphincter Of Oddi ◽

Nerve Fibers ◽

Excitatory Postsynaptic Potential ◽

Inhibitory Neurotransmitter ◽

Fiber Tracts ◽

Inhibitory Postsynaptic Potentials ◽

Dopamine Beta Hydroxylase ◽

Staining Techniques ◽

The Uk ◽

Ganglionated Plexus

Intracellular recording and immunohistochemical staining techniques were used to establish whether sphincter of Oddi (SO) ganglia are a target of sympathetic input to this region. Norepinephrine (0.01-10.0 microM) decreased the amplitude of the nicotinic fast excitatory postsynaptic potential (EPSP) evoked by stimulation of interganglionic fiber tracts, with a half-maximal inhibitory concentration (EC50) of 300 nM. Norepinephrine did not alter the responsiveness of the neurons to acetylcholine. The alpha 2-adrenoreceptor agonist UK-14304 mimicked the norepinephrine-induced effect with a EC50 of 2.5 nM, whereas alpha 1- and beta-adrenoreceptor agonists had no effect on the EPSP. The alpha 2-adrenoreceptor antagonist idazoxan (1.0 microM) inhibited the UK-14304 response, with a dissociation constant of 1.0 nM. Release of endogenous catecholamines, by the addition of tyramine (100 microM) to the bath, caused an idazoxan-sensitive decrease in the amplitude of the fast EPSP. In the minority of SO neurons that exhibited inhibitory postsynaptic potentials (IPSPs), norepinephrine caused a hyperpolarization of the membrane potential. The IPSP and the norepinephrine-induced hyperpolarization were inhibited by alpha 2-adrenoreceptor antagonists. Desipramine (1.0 microM), an uptake inhibitor, reversibly increased the amplitude of the IPSP. Immunoreactivities for tyrosine hydroxylase and dopamine beta-hydroxylase were coexistent in nerve fibers and nonexistent in cell bodies in the ganglionated plexus of the SO. The results of this study indicate that norepinephrine acts pre- and postsynaptically as an inhibitory neurotransmitter in SO ganglia.

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Immunohistochemical Characterization of Canine Transmissible Venereal Tumor

Veterinary Pathology ◽

10.1177/030098589603300301 ◽

1996 ◽

Vol 33 (3) ◽

pp. 257-263 ◽

Cited By ~ 64

Author(s):

E. Mozos ◽

A. Méndez ◽

J. C. Gómez-Villamandos ◽

J. Martín de las Mulas ◽

J. Pérez

Keyword(s):

Light Chain ◽

B Lymphocyte ◽

Protein S ◽

Lambda Light Chain ◽

Formalin Fixed Paraffin ◽

S 100 ◽

Formalin Fixed Paraffin Embedded ◽

Progressive Growth ◽

Staining Techniques ◽

Alpha 1 Antitrypsin

The collective immunohistochemical expression of human lysozyme, human alpha-1-antitrypsin, human CD3 antigen, calf vimentin, human keratins, human lambda light chains, canine immunoglobulins IgG, IgM, and bovine protein S-100 has been analyzed on formalin-fixed, paraffin-embedded tissue sections of 25 spontaneous canine transmissible venereal tumors (CTVT) from both genital and extragenital locations using the avidin-biotin-peroxidase complex technique. Lysozyme immunoreactivity was detected in 10/25 CTVT, alpha-1-antitrypsin in 14/25 CTVT, and vimentin in 25/25 CTVT. All CTVT cells were negative to keratins 5 + 8 of the Moll catalogue (RCK-102), S-100 protein, lambda light-chain immunoglobulins, IgG, IgM, and CD3 antigen. The intratumoral T- and B-lymphocyte infiltrate was differentiated using CD3 antigen, lambda light-chain immunoglobulins, IgG, and IgM, and this technique could be useful to evaluate the regressive or progressive growth stage of venereal tumors. Our findings support the hypothesis of a histiocytic immunophenotype for CTVT, and these staining techniques could be used in the differential diagnosis with lymphomas.

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