Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells

Author(s):  
Guy Echalier
1950 ◽  
Vol 27 (1) ◽  
pp. 29-39 ◽  
Author(s):  
GARTH CHAPMAN

Four aspects of the functioning of a fluid-filled cylindrical animal have been examined, viz.: (I) the role of the body fluid as a skeleton for the interaction of the longitudinal and circular muscles of which the animal must be composed; (2) the measurement of the maximum thrust which the animal can exert by measurement of its internal hydrostatic pressure; (3) the application of the force to the substratum and the part played by friction; (4) the relation between the changes in dimensions of the animal and the working length of the muscles. Under (1) the necessity for a longitudinal and circular construction has been shown and the necessity for a closed system emphasized. Under (2) the pressure exerted on the body fluid by the contraction of the longitudinal and circular muscles is discussed, and from their cross-sectional areas it is shown to be probable that when contracting maximally in Lumbricus they are not balanced, but that the longitudinals are about ten times as strong as the circulars. Under (3) it is shown that the strength of an animal as measured by its internal hydrostatic pressure is sufficient to account for its customary activities. Use which may be made of the longitudinals during burrowing is pointed out. Under (4) it is shown to be mechanically sound for burrowing animals of cylindrical form to be ‘fat’, but that a ‘thin’ animal is more efficient at progression.


1952 ◽  
Vol 29 (1) ◽  
pp. 22-29
Author(s):  
A. D. HOBSON ◽  
W. STEPHENSON ◽  
A. EDEN

The results obtained in this investigation are admittedly not as extensive as is desirable but they allow certain conclusions to be drawn. 1. The sodium and potassium contents of the body fluid of Ascaris lumbricoides are somewhat variable, but these variations do not seem to be dependent upon those of the external medium. 2. The calcium and magnesium contents of the body fluid are relatively constant and are not affected by those of the external medium. 3. The chloride concentration of the body fluid is closely related to and always remains lower than that of the external medium. 4. As shown in Table 2, there is a large gap between the total concentrations of inorganic cations and anions in the intestinal fluid of the pig. Presumably a considerable proportion of the inorganic cations are combined with organic anions, at present undetermined. Exposing the worms to saline media composed of chloride caused a large rise in the internal chloride concentration. This may well be a limiting factor in the life of the animals in such media, and the next step forward would seem to be the fuller analysis of the environment to which they are normally exposed.


2020 ◽  
Vol 8 (9) ◽  
pp. 4426-4430
Author(s):  
Bhuvnesh Sharma ◽  
Gyanendra Datta Shukla ◽  
Parul Sharma

Kidneys plays a vital role in homeostasis by maintaining the body fluid and removing the harmful toxins out of the body through urine formation. Chronic kidney disease is a type of kidney disease which is pro-gressive in nature and there is gradual loss of kidney function over a period of months or years and have complex and different etio-pathologies. With the growing number of cases and keeping in mind the availa-ble conventional line of treatment and its financial burden on patient, Ayurveda through its holistic line of management, either in the form of Shamana & Shodhana or in the form of dietary advices stand distinct and it seems to be effective and safe. And by including proper daily dietary rules and regimens in patients of Chronic kidney disease (CKD) it can be very effectively managed. Here Trinpanchmool, Punarnava, Gokshur used in Basti. Basti plays a vital role here in CKD, it helps in expelling out the uremic toxins which originates in gut thereby improving the quality of life and reducing the sign and symptoms of dis-ease.


2021 ◽  
Author(s):  
Mingfeng Zhou ◽  
Yichao Ou ◽  
Guangsen Wu ◽  
Kai Li ◽  
Junjie Peng ◽  
...  

Background: Hypothalamic injury causes several complicated neuroendocrine-associated disorders, such as water-electrolyte imbalance, obesity, and hypopituitarism. Among these, central diabetes insipidus (CDI), characterized by polyuria, polydipsia, low urine specific gravity, and deficiency of arginine vasopressin contents, is a typical complication after hypothalamic injury. Methods: CDI was induced by hypothalamic pituitary stalk injury in male animals. Behavioral parameters and blood sample were collected to evaluate the characteristics of body fluid metabolism imbalance. The brains were harvested for high-throughput RNA sequencing and immunostaining to identify pathophysiological changes in corresponding hypothalamic nuclei. Results: Based on transcriptomic analysis, we demonstrated the upregulation of the Atf3/c-Jun axis and identified Lgals3, a microglial activation related gene, as the most significant target gene in response to the body fluid imbalance in CDI. Furthermore, we found that the microglia possessed elevated phagocytic ability, which could promote the elimination of arginine vasopressin neurons after hypothalamic injury. Conclusion: Our findings suggested that the Atf3/c-Jun/Lgals3 axis was associated with the microglial activation, and might participate in the loss of functional arginine vasopressin neurons in CDI after hypothalamic injury.


1928 ◽  
Vol 6 (1) ◽  
pp. 1-11
Author(s):  
J. G. H. FREW

In vitro tissue culture Is shown to be a possible mode of experimentation with the tissues of the Blow Fly larva. Methods are described- whereby the tissues, and the body fluids requisite as culture media may be obtained free from bacteria. The imperfections of the technique are noted and the conclusion reached that a successful technique must depend on the rearing of bacteria-free larvae, for which a method Is briefly outlined. It Is shown that progress in this part of the work must await further physiological knowledge, particularly in respect to the nature of the body fluids.


1913 ◽  
Vol 18 (5) ◽  
pp. 584-590
Author(s):  
L. J. Gillespie

1. Pneumococci, when freshly isolated from the body, are able to live and multiply when a small number of them are inoculated into a small amount of broth. If, however, the inoculations are made in large amounts of broth, many more bacteria must be inoculated in order that they may grow. 2. It requires much smaller numbers of pneumococci to start a growth on agar than are required to start a growth in broth. 3. This predilection for solid medium disappears when the bacteria are grown for some time outside the body. 4. This phenomenon is not dependent on differences in chemical composition between the two media employed or on the presence of more available oxygen in one case than in the other. 5. It is probably dependent entirely on physical differences in the two kinds of media, and bears some relation to the differences in possibilities for diffusion in the two media.


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