Learning (by) osmosis: an approach to teaching osmolarity and tonicity

Understanding osmolarity and tonicity is one of the more challenging endeavors undertaken by students of the natural sciences. We asked students who completed a course in animal physiology to submit an essay explaining what they found most perplexing about this subject, and what in-class activities proved most useful to them. Students had difficulty distinguishing osmolarity from tonicity and determining tonicity based on the solution’s composition. The most useful activities were questions requiring simultaneous consideration of both osmolarity and tonicity. Problems that require calculating osmotic concentration and the volumes of body fluid compartments after administration or loss of various solutions emphasize the significance of osmolarity and tonicity in the context of systemic homeostasis and clinical medicine. We hope that our approach to teaching osmolarity and tonicity will prove useful to physiology lecturers who are looking for new ways of introducing this complicated topic to their health professions students.

Kidney and body fluids

The physiology of body fluid compartments is revised in association with clinical assessment of fluid balance and the management of fluid-related abnormalities. Electrolyte and acid-base disorders; causes, consequences and management are summarized. Acute kidney injury in the context of perioperative medicine is discussed; including definitions, risks, causes, recognition, prevention and preventative measures. Renal replacement therapy strategies are explained.

Determining dehydration and its compartmentation in horses at rest and with exercise: a concise review and focus on multi-frequency bioelectrical impedance analysis

Multi-frequency bioelectrical impedance analysis (MFBIA) has been, and likely will increasingly be, used to rapidly and non-invasively assess the time course of volume losses and recovery in horses. Dehydration in performance horses is frequently the cause of health and performance problems, and presently used techniques for objectively quantifying optimum hydration are time consuming and challenging to perform accurately. Dehydration can take a number of different forms, with a balanced loss of water and electrolytes from both extra- and intracellular fluid compartments, or a primarily extracellular or intracellular dehydration. This review summarises the current state of knowledge regarding the quantification of dehydration, losses of water and electrolytes from extra- and intracellular fluid compartments. The effects of dehydration on exercise performance, muscle function, cardiovascular function, thermoregulation and feeding are briefly summarised. The review provides a quantitative description of the magnitude and time course of compartmental fluid losses and recovery in horses in response to feeding and due to exercise at different intensities and durations representing the endurance horse to the track race horse. Effective rehydration requires knowledge of the losses from the main body fluid compartments, which is now possible using MFBIA technology. The present review outlines the key approaches that have been used to assess dehydration in horses, including the new technique of MFBIA.