Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

2015 ◽  
pp. 1-46 ◽  
Author(s):  
Elizabeth A. Worrall ◽  
Francis O. Wamonje ◽  
Gerardine Mukeshimana ◽  
Jagger J.W. Harvey ◽  
John P. Carr ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Necrosis Virus

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

2005 ◽  
Vol 95 (9) ◽  
pp. 1037-1042 ◽  
Author(s):  
Richard C. Larsen ◽  
Phillip N. Miklas ◽  
Keri L. Druffel ◽  
Stephen D. Wyatt
Keyword(s):  
Amino Acids ◽  
Mosaic Virus ◽  
Molecular Size ◽  
Bean Common Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Virus ◽  
Bean Plants ◽  
Recombination Point ◽  
Russian Strain ◽  
Polymerase Chain

A strain of Bean common mosaic necrosis virus (BCMNV) from Idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the NL-3 D strain (of Drifjhout) by reaction of differential bean cultivars. However, this BCMNV strain (designated NL-3 K) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. The nucleotide sequence encoding the predicted polyprotein of NL-3 K was 9,893 nucleotides (nt) in length, yielding a peptide with a molecular size of 362.1 kDa compared with a 9,626-nt, 350.9-kDa polyprotein for NL-3 D. Sequence analysis of the putative P1 protein suggests that the NL-3 K strain is a recombinant between NL-3 D and the Russian strain (RU1) of Bean common mosaic virus. The P1 protein of NL-3 K consisted of 415 amino acids compared with 317 for NL-3 D. The first 114 predicted amino acids of the NL-3 K P1 region were 98% identical with RU1. The remaining 301 amino acids of the protein shared only 34% identity with RU1 but were 98% identical with NL-3 D. Primers were designed that flanked the recombination point in the P1 coding sequence of NL-3 K. An amplicon of the expected size was produced by reverse-transcriptase polymerase chain reaction of total nucleic acid extracts of bean plants inoculated with NL-3 K, but not from those with NL-3 D or RU1. The increased symptom severity on selected common bean lines induced by NL-3 K suggests that the P1 gene may play a significant role in pathogenicity and virulence.

scholarly journals Bean common mosaic virus and Bean common mosaic necrosis virus in Mexico

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 21-25 ◽  
Author(s):  
N. Flores-Estévez ◽  
J. A. Acosta-Gallegos ◽  
L. Silva-Rosales
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Bean Common Mosaic Virus ◽  
Nucleotide Sequences ◽  
Necrosis Virus ◽  
Cp Gene

A survey was performed in Mexico to study the distribution of Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) using a set of primers directed to the coat protein gene (CP) that were designed to detect and characterize the two viral species. Both viral species were present in different locations in the country. BCMV was predominant in the central states of the country, whereas BCMNV proliferated toward the eastern tropical states. The alignment of nine nucleotide sequences for each viral species at the amino region of the CP gene confirmed the identities of the viruses and set the basis to assign them tentatively to pathogroups I, II, and VI.

scholarly journals Occurrence and distribution of viruses infecting the bean in Serbia

2010 ◽  
Vol 62 (3) ◽  
pp. 595-601 ◽  
Author(s):  
Dragana Petrovic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
Milka Vujakovic ◽  
Mirjana Vasic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Viral Infections ◽  
Alfalfa Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Pcr Method ◽  
Virus Isolates ◽  
Viral Isolates

This work describes the incidence and distribution of the most important bean viruses in Serbia: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The viral isolates were characterized serologically and biologically. BCMV was found in the largest number of plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), and AMV (3.41%), since BYMV was not determined. Mixed viral infections were found in several samples. The RT-PCR method was used to prove that the tested isolates belong to the BCMV, family Potyviridae and strains Russian and NL-3 D. Results obtained in this work will enable further studies of the genetic variability of bean virus isolates from Serbia. .

scholarly journals Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus

2021 ◽  
Vol 20 (6) ◽  
pp. 7-18
Author(s):  
İlyas Deligoz ◽  
Miray Arlı-Sökmen ◽  
Mucella Tekeoglu
Keyword(s):  
Mosaic Virus ◽  
Agricultural Research ◽  
Bean Common Mosaic Virus ◽  
Virus Species ◽  
Necrosis Virus ◽  
Dry Bean ◽  
Breeding Lines ◽  
Bean Plants ◽  
Resistant Lines ◽  
Recessive Genes

Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are among the most economically important virus species infecting common bean. The use of resistant plant cultivars is the most effective way to control these viruses. National dry bean breeding studies have been conducted by seven different governmental agricultural research institutes in Turkey, and advanced breeding lines have been developed by using the selected local dry bean populations and crossing studies. In this study, 204 breeding lines were tested for resistance levels to BCMV and BCMNV. Initially, BCMNV NL-3 and BCMV NL-4 strains were individually sap-inoculated onto the leaves of bean plants belonging to each breeding lines with 10 replications, and the reactions of plants were evaluated for symptomatic appearance of virus infection 21 days after inoculation. Additionally, phenotypic evaluation was confirmed by molecular markers linked to resistance genes. As a result of the study, 153 breeding lines were found to involve the dominant I gene whereas four and five of the tested lines had the recessive genes bc-1² and bc-2², respectively. In conclusion, it was emphasized that these breeding lines could be registered after evaluating them in terms of yield and quality. Also, the use of seeds of the resistant lines to supply the source of virus-resistance in breeding studies and maintaining their seeds at the national genebank were recommended.

scholarly journals Inheritance of Resistance to the NL-8 Strain of Bean Common Mosaic Necrosis Virus in Bean

HortScience ◽  
2001 ◽  
Vol 36 (5) ◽  
pp. 958-960 ◽  
Author(s):  
R. Provvidenti
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
The Other ◽  
Dominant Factor ◽  
Susceptible Cultivar ◽  
Inheritance Of Resistance ◽  
Necrosis Virus ◽  
Backcross Populations ◽  
Reciprocal Backcross

Bean common mosaic necrosis virus (BCMNV) includes four African strains, BCMNV-NL3, -NL-5, -NL8, and -TN1, previously considered to be members of the bean common mosaic virus (BCMV) group. Many bean cultivars resistant to BCMNV-NL8 were found to be susceptible to the other strains of the virus. `California Light Red Kidney' (CLRK) and `Carbon', resistant to BCMNV-NL8, were crossed with the susceptible cultivars Sanilac or Black Turtle 2 (BT-2). In plants of F1, F2, and reciprocal backcross populations involving CLRK × `Sanilac' or BT-2 × `Carbon', the resistance to BCMVN-NL8 was determined to be conferred by a single dominant factor. The same factor was detected in BCMNV-NL8-resistant `Great Northern 1140' and `IVT-7214, when crossed with the susceptible cultivar Stringless Refugee or BT-2.

scholarly journals Comprehensive Surveys of Bean common mosaic virus and Bean common mosaic necrosis virus and Molecular Evidence for Occurrence of Other Phaseolus vulgaris Viruses in Tanzania

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2361-2370 ◽  
Author(s):  
Beatrice Mwaipopo ◽  
Susan Nchimbi-Msolla ◽  
Paul J. R. Njau ◽  
Deogratius Mark ◽  
Deusdedith R. Mbanzibwa
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Molecular Evidence ◽  
Coat Proteins ◽  
Necrosis Virus ◽  
Virus Diseases

Virus diseases are among the main biotic factors constraining common bean (Phaseolus vulgaris L.) production in Tanzania. Disease management requires information on types, distribution, incidence, and genetic variation of the causal viruses, which is currently limited. Thus, a countrywide comprehensive survey was conducted. Use of a next-generation sequencing technique enabled simultaneous detection of 15 viruses belonging to 11 genera. De novo assembly resulted in many contigs, including complete or nearly complete sequences of Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), and Southern bean mosaic virus (SBMV). Some viruses (for example, SBMV and Tomato leaf curl Uganda virus-related begomovirus) were detected for the first time in common bean in Tanzania. Visually assessed virus-like disease incidence ranged from 0 to 98% but reverse-transcription polymerase chain reaction-based incidence of BCMV and BCMNV (7,756 samples) was mostly less than 40%. The Sanger-based nucleotide sequences encoding coat proteins of BCMV and BCMNV isolates were 90.2 to 100% and 97.1 to 100% identical to each other, respectively. Phylogenetic analysis showed that BCMV isolates were more diverse than BCMNV isolates. The information generated in this study will contribute to the development of molecular diagnostic tools and strategies for management of virus diseases nationally and internationally. [Formula: see text] Copyright © 2018 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

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