Chronic hypoxia increases IL-6, TNF-, and matrix metalloproteinases (MMP2 and MMP9) expression of fetal guinea pig hearts

2006 ◽  
Vol 195 (6) ◽  
pp. S9 ◽  
Author(s):  
Chien Oh ◽  
Yafeng Dong ◽  
Loren Thompson
Keyword(s):  
Matrix Metalloproteinases ◽  
Guinea Pig ◽  
Chronic Hypoxia ◽  
Mmp9 Expression

scholarly journals Chronic hypoxia increases peroxynitrite, MMP9 expression, and collagen accumulation in fetal guinea pig hearts

2011 ◽  
Vol 71 (1) ◽  
pp. 25-31 ◽  
Author(s):  
LaShauna C. Evans ◽  
Hongshan Liu ◽  
Gerard A. Pinkas ◽  
Loren P. Thompson
Keyword(s):  
Guinea Pig ◽  
Chronic Hypoxia ◽  
Mmp9 Expression ◽  
Collagen Accumulation

scholarly journals Chronic Hypoxia Impairs Cytochrome Oxidase Activity Via Oxidative Stress in Selected Fetal Guinea Pig Organs

2012 ◽  
Vol 20 (3) ◽  
pp. 299-307 ◽  
Author(s):  
Yazan M. Al-Hasan ◽  
LaShauna C. Evans ◽  
Gerard A. Pinkas ◽  
Erinne R. Dabkowski ◽  
William C. Stanley ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Guinea Pig ◽  
Cytochrome Oxidase ◽  
Oxidase Activity ◽  
Chronic Hypoxia ◽  
Cytochrome Oxidase Activity

Abstract WP298: Matrix Metalloproteinases Are Involved In The Regulation Of Angiogenesis After Intracerebral Hemorrhage

Stroke ◽  
2013 ◽  
Vol 44 (suppl_1) ◽  
Author(s):  
Qi-Ting Liu ◽  
Han-Jin Cui ◽  
Jie-Kun Luo ◽  
Yuan Lin ◽  
TAO TANG
Keyword(s):  
Matrix Metalloproteinases ◽  
Intracerebral Hemorrhage ◽  
Western Blot ◽  
Sham Group ◽  
Treated Group ◽  
Barrier Dysfunction ◽  
Mmp Inhibitor ◽  
Neurological Severity Score ◽  
Mmp9 Expression ◽  
The Times

Background and purpose: Intracerebral hemorrhage (ICH) is one of the most devastating subtypes of stroke. And our previous work has demonstrated that ICH induces angiogenesis, accompanied by up-regulation of pro-angiogenic factors. Matrix metalloproteinases (MMPs) can cause blood-brain barrier dysfunction by degrading the extracellular cellular matrix (ECM) around the vessels after ICH, but opening a way for the prolonging newborn vessels is a key step for their functional structure, therefore, the purpose of the study is to investigate whether MMPs are involved in the process of angiogenesis after ICH. Methods: Thirty Kunming mice were randomly divided into sham group, ICH group and doxycycline (DOX)-treated group. And then 5 mice were randomly selected for Western Blot to detect the expression of MMP9, and the other five for the immunohistochemistry to detect vWF. ICH model was induced by injection collagenase type VII into right globus pallidus stereotaxically, and DOX, a broad-spectrum MMP inhibitor, was injected by intraperitoneally at 7 days after ICH induction. Neurological severity score (NSS), corner turn test and foot-fault test were used to investigate the neurological function. And vWF-positive vessels were counted around the hematoma. Results: At 7 days, there is no difference between the two ICH-induced groups in NSS, corner turn test, foot-fault test; while at 14 days, the NSS in ICH group is significantly lower than that of DOX-treated group ( P <0.05), and the times for right-turn and foot-fault in ICH group are notably fewer than those of DOX-treated group ( P <0.05); At 14d, the number of vWF-postive microvessel in ICH group was significantly larger than that of DOX-treated group ( P <0.01), and Western Blot revealed that DOX decreased MMP9 expression remarkably( P <0.01). Conclusion: Matrix metalloproteinases were involved in the regulation of angiogenesis after ICH.

scholarly journals In vitro hypoxia increases production of matrix metalloproteinases and tryptase in isolated rat lung mast cells

2008 ◽  
pp. 903-910
Author(s):  
H Maxová ◽  
J Novotná ◽  
L Vajner ◽  
H Tomášová ◽  
R Vytášek ◽  
...  
Keyword(s):  
Mast Cells ◽  
Matrix Metalloproteinases ◽  
Chronic Hypoxia ◽  
Pulmonary Arteries ◽  
Collagenolytic Activity ◽  
Interstitial Tissue ◽  
Vessel Remodeling ◽  
Immunohistological Staining ◽  
Isolated Rat Lung

Chronic hypoxia results in hypoxic pulmonary hypertension characterized by fibrotization and muscularization of the walls of peripheral pulmonary arteries. This vessel remodeling is accompanied by an increase in the amount of lung mast cells (LMC) and the presence of small collagen cleavage products in the vessel walls. We hypothesize that hypoxia activates LMC, which release matrix metalloproteinases (MMPs) cleaving collagen and starting increased turnover of connective tissue proteins. This study was designed to determine whether in vitro hypoxia stimulates production of MMPs in rat LMC and increases their collagenolytic activity. The LMC were separated on the Percoll gradient and then were divided into two groups and cultivated for 24 h in 21 % O(2) + 5 % CO(2) or in 10 % O(2) + 5 % CO(2). Presence of the rat interstitial tissue collagenase (MMP-13) in LMC was visualized by immunohistological staining and confirmed by Western blot analysis. Total MMPs activity and tryptase activity were measured in both cultivation media and cellular extracts. Exposure to hypoxia in vitro increased the amount of cells positively labeled by anti-MMP-13 antibody as well as activities of all measured enzymes. The results therefore support the concept that LMC are an important source of increased collagenolytic activity in chronic hypoxia.

scholarly journals Chronic hypoxia differentially increases glutathione content and γ-glutamyl cysteine synthetase expression in fetal guinea pig organs

2008 ◽  
Vol 84 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Chien Oh ◽  
Yafeng Dong ◽  
Christopher Harman ◽  
Hugh E. Mighty ◽  
Jerome Kopelman ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Chronic Hypoxia ◽  
Cysteine Synthetase

scholarly journals Effects of N-Acetyl Cysteine on the Expression of Matrix Metalloproteinases 2 and 9 in the Lung Tissue of Rats Exposed to Cadmium

2020 ◽  
Keyword(s):  
Single Dose ◽  
Matrix Metalloproteinases ◽  
Lung Tissue ◽  
Continuous Exposure ◽  
Tissue Samples ◽  
Cd Toxicity ◽  
Mmp9 Expression ◽  
The Matrix ◽  
Continuous Dose

Objectives: This study aimed to investigate the effect of cadmium (Cd) on the matrix metalloproteinases (MMPs) -2 and -9 expression in the lung, and the role of N-acetylcysteine (NAC) in preserving the lung cells against Cd toxicity. Methods: The rats were randomly divided into five groups of G1 (control), G2 (single dose of Cd), G3 (continuous dose of Cd), G4 (single dose of Cd+NAC), and G5 (continuous dose of Cd+NAC). The level of Cd in the blood and lung tissue was measured by atomic absorption spectroscopy. Moreover, the expression of MMP2 and MMP9 genes was considered using RT-PCR. Results: Single and continuous exposure to Cd caused a significant increase in serum and the lung tissue of Cd in G2 (0.23±0.04 mg/L and 0.35±0.047 μg/g tissue) and G3 (0.50±0.068 mg/L and 0.81±0.063 μg/g tissue) groups, compared to other groups (P<0.001). The NAC supplementation significantly decreased Cd levels in the serum and lung tissue samples of rats exposed to single or continuous Cd (P<0.001). Furthermore, exposure to a single and continuous dose of Cd caused a significant increase in the MMP2 expression by 3.24-fold (P=0.003) and 11.9-fold (P<0.001), respectively. Additionally, single and continuous dose treatment of Cd led to a significant increase in the MMP9 expression by 3.20-fold (P=0.004) and 7.54-fold (P<0.001), respectively. The NAC treatments decreased the expression of MMP2 and MMP9 in the lung of rats exposed to single or continuous Cd. Conclusion: The Cd exposure was strongly associated with the accumulation of Cd and overexpression of MMP2 and MMP9 in the lung tissue. Moreover, the NAC can protect the lungs against Cd toxicity by decreasing Cd and down-regulating MMPs.

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