MALDI-TOF MS for rapid diagnosis of Anaerobiospirillum succiniciproducens, an unusual causative agent of bacteraemia in humans. Two case reports and literature review

Anaerobe ◽  
2019 ◽  
Vol 55 ◽  
pp. 130-135 ◽  
Author(s):  
Marina Alguacil-Guillen ◽  
Luis Ramos-Ruperto ◽  
Juan Carlos Ramos Ramos ◽  
Angel Robles-Marhuenda ◽  
Julio García-Rodriguez ◽  
...  
Keyword(s):  
Literature Review ◽  
Causative Agent ◽  
Case Reports ◽  
Rapid Diagnosis ◽  
Maldi Tof Ms ◽  
Anaerobiospirillum Succiniciproducens ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Identification of cereulide producingBacillus cereusby MALDI-TOF MS

2018 ◽  
Author(s):  
Sebastian Ulrich ◽  
Christoph Gottschalk ◽  
Richard Dietrich ◽  
Erwin Märtlbauer ◽  
Manfred Gareis
Keyword(s):  
Causative Agent ◽  
Food Chain ◽  
Reliable Method ◽  
Correct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Heat Stable ◽  
Food Borne ◽  
Reliable Methods ◽  
Tof Ms

AbstractTheBacillus(B.)cereusgroup is genetically highly homogenous and consists of nine recognized species which are present worldwide.B. cereussensu stricto play an important role in food-borne diseases by producing different toxins. Yet, only a small percentage ofB. cereusstrains are able to produce the heat stable depsipeptide cereulide, the causative agent of emetic food poisonings. To minimize the entry of emeticB. cereusinto the food chain, food business operators are dependent on efficient and reliable methods enabling the differentiation between emetic and non-emetic strains. Currently, only time-consuming cell bioassays, molecular methods and tandem mass spectrometry are available for this purpose. Thus, the aim of the present study was to establish a fast and reliable method for the differentiation between emetic and non-emetic strains by MALDI-TOF MS. Selected isolates/strains of theB. cereusgroup (total n=110, i.e. emetic n=45, non-emetic n=65) were cultured on sheep blood agar for 48h.Subsequently, the cultures were directly analyzed by MALDI-TOF MS without prior extraction steps (direct smear method). The samples were measured in linear positive ionization mode in the mass range ofm/z800 - 1,800 Da. Using ClinProTools 3.0 statistical software and flex analyst, a differentiation between emetic and non-emetic isolates was possible with a rate of correct identification of 99.1 % by means of the evaluation of two specific biomarkers (m/z1171 and 1187 Da).ImportanceBacillus(B.)cereusplays an important role in food-borne diseases due to the production of different toxins, e.g. the heat stable depsipeptide cereulide. Only a small number ofB. cereusstrains are able to produce this toxin, the causative agent of emetic food poisonings. To minimize the entry of emeticB. cereusinto the food chain, food business operators require efficient and reliable methods enabling the differentiation between emetic and non-emetic strains. The aim of the present study was to develop a fast and reliable method for the differentiation between emetic and non-emetic strains by MALDI-TOF MS. A differentiation between emetic and non-emetic isolates was possible with a rate of correct identification of 99.1 % by means of the evaluation of two specific biomarkers (m/z1171 and 1187 Da).

scholarly journals APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT

2017 ◽  
pp. 9-17
Author(s):  
D. V. Ulshina ◽  
D. A. Kovalev ◽  
D. G. Ponomarenko ◽  
D. V. Rusanova ◽  
N. M. Shvetsova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Causative Agent ◽  
Brucella Melitensis ◽  
Time Of Flight ◽  
Protein Profiling ◽  
Blood Samples ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Aim.Study the possibility to apply time-of-flight mass-spectrometry for detection of causative agent of brucellosis in blood. Materials and methods. Brucella strains: 5 Brucella melitensis and 21 Brucella abortus. Protein profiling in linear mode on MALD1-TOF mass-spectrometer Microflex «Bruker Daltonics». Results. Technique for disinfection and preparation of blood samples was modified and optimized for MALDI-TOF MS analysis. 120 representative protein profiles of sera extract were obtained that contain brucellosis causative agent. A resulting peak-list (super-spectrum) of the studied protein fraction of blood extract of a conditionally healthy human within the studied group was formed and analyzed. Conclusion. A scheme of brucella detection in blood samples by MALDI-TOF MS is proposed, based on detection of a complex of 15 genus-specific fragments. Signals on mass-spectra of extracts of leukocyte fraction of blood, artificially contaminated with brucellosis causative agents are characterized.

scholarly journals A case of CAPD peritonitis in which Streptococcus vestibularis was found to be the causative agent by MALDI‒TOF MS

2021 ◽  
Vol 54 (10) ◽  
pp. 523-528
Author(s):  
Junko Ukai ◽  
Yukihiro Kimura ◽  
Tomomi Tomita ◽  
Kotono Kobori ◽  
Suzuka Kawamura ◽  
...  
Keyword(s):  
Causative Agent ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals STUDY OF THE MASS-SPECTORS’ FEATURES OF SPORES AND VEGETATIVE FORMS OF BACILLUS ANTHRACIS BY THE METHOD OF TIME OF FLIGHT MASS-SPECTROMETRY

2018 ◽  
pp. 66-72
Author(s):  
D. V. Ulshina ◽  
E. I. Eremenko ◽  
D. A. Kovalev ◽  
A. G. Ryazanova ◽  
I. V. Kuznetsova ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Causative Agent ◽  
Protein Profiling ◽  
Protein Profiles ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Vegetative Form ◽  
Maldi Tof Mass Spectra ◽  
Tof Ms

Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.

MALDI-TOF MS in Anaerobiospirillum succiniciproducens bacteremia: A report of 4 cases in different hosts

Anaerobe ◽  
2018 ◽  
Vol 54 ◽  
pp. 267-270 ◽  
Author(s):  
B. Fox ◽  
M.A. Berger ◽  
M. Roncallo ◽  
L. Pinoche ◽  
M.E. Ibáñez ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Anaerobiospirillum Succiniciproducens ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Enterococcus hirae bacteremia associated with acute pyelonephritis in a patient with alcoholic cirrhosis: a case report and literature review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tomoaki Nakamura ◽  
Kazuhiro Ishikawa ◽  
Takahiro Matsuo ◽  
Fujimi Kawai ◽  
Yuki Uehara ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Literature Review ◽  
Urinary Tract Infections ◽  
Alcoholic Cirrhosis ◽  
Enterococcus Hirae ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tract Infections ◽  
Tof Ms

Abstract Background Infections caused by Enterococcus hirae are common in animals, with instances of transmission to humans being rare. Further, few cases have been reported in humans because of the difficulty in identifying the bacteria. Herein, we report a case of pyelonephritis caused by E. hirae bacteremia and conduct a literature review on E. hirae bacteremia. Case presentation A 57-year-old male patient with alcoholic cirrhosis and neurogenic bladder presented with fever and chills that had persisted for 3 days. Physical examination revealed tenderness of the right costovertebral angle. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of the patient’s blood and urine samples revealed the presence of E. hirae, and pyelonephritis was diagnosed. The patient was treated successfully with intravenous ampicillin followed by oral linezolid for a total of three weeks. Conclusion The literature review we conducted revealed that E. hirae bacteremia is frequently reported in urinary tract infections, biliary tract infections, and infective endocarditis and is more likely to occur in patients with diabetes, liver cirrhosis, and chronic kidney disease. However, mortality is not common because of the high antimicrobial susceptibility of E. hirae. With the advancements in MALDI-TOF MS, the number of reports of E. hirae infections has also increased, and clinicians need to consider E. hirae as a possible causative pathogen of urinary tract infections in patients with known risk factors.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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