Inhibitory effect of Platycodon grandiflorum on TH1 and TH2 immune responses in a murine model of 2,4-dinitrofluorobenzene–induced atopic dermatitis–like skin lesions

2011 ◽  
Vol 106 (1) ◽  
pp. 54-61 ◽  
Author(s):  
Min-Soo Kim ◽  
Yun-Gyung Hur ◽  
Wan-Gi Kim ◽  
Byoung-Woo Park ◽  
Kyoo-Seok Ahn ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immune Responses ◽  
Murine Model ◽  
Skin Lesions ◽  
Inhibitory Effect ◽  
Platycodon Grandiflorum ◽  
Th1 And Th2

scholarly journals Acupoint Autohemotherapy Attenuates DNCB-induced Atopic Dermatitis Lesions by Regulating Th1/Th2 Cytokine Balance in BALB/c Mice

2021 ◽  
Author(s):  
Zhiwen Zeng ◽  
Jinquan Huang ◽  
Yong Chen ◽  
Xiao Yu ◽  
Wei Zhu ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immune Responses ◽  
Immunohistochemical Analysis ◽  
Skin Lesions ◽  
Inhibitory Effect ◽  
Serum Ige ◽  
Cytokine Balance ◽  
Autologous Whole Blood ◽  
Similar Inhibitory Effect ◽  
Ifn Γ

Abstract Objective Acupoint autohemotherapy (A-AHT) is considered an effective therapy for atopic dermatitis (AD) with few side-effects. Previous experiments showed the treatment had the potential to regulate T helper (Th) 1 and Th2 cytokines, like interferon (IFN)- gamma and interleukin (IL)- 4. This study focuses on the effects of A-AHT on the AD-like skin lesions through regulating Th1/Th2 immune responses. Methods The treatments of A-AHT, sham acupoint autohemotherapy and acupoint injection of normal saline were administered in the AD mice once every other day for 4 weeks. The total immunoglobulin (Ig) E, IL-4 and IFN-γ cytokine levels in the serum were examined after animal sacrifice. Th1/Th2 expression was analyzed in murine spleen cells via flow cytometry and immunohistochemical analysis of GATA-3 and T-bet in skin lesions were further assessed. Results Either type of repeated autologous whole blood (AWB) injection (into acupoint or sham acupoint) reduced the severity of AD-like symptoms and level of serum IgE. All of the three treatments had the similar inhibitory effect on levels of IL-4 and upregulation on the ratio of IFN-γ/IL-4, while differed on Th1/Th2 ratio as A-AHT regulates the body’s Th1/Th2 shift. This treatment also increased the related transcription factors T-bet expression, and upregulated T-bet/GATA3 ratio compared with the DNCB group. These differences were significant only in A-AHT group. Conclusion A-AHT effectively reduces AD symptoms and serum IgE levels in a mouse model and may act by regulating Th1/Th2 immune responses.

Immunostimulatory Sequence CpG Elicits Th1-Type Immune Responses in Inflammatory Skin Lesions in an Atopic Dermatitis Murine Model

2008 ◽  
Vol 147 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Guoying Wang ◽  
Nanna Fyhrquist-Vanni ◽  
Henrik Wolff ◽  
Marie-Caroline Dieu-Nosjean ◽  
Lajos Kemeny ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immune Responses ◽  
Murine Model ◽  
Skin Lesions ◽  
Inflammatory Skin

scholarly journals CURRENT CONCEPTS OF ATOPIC DERMATITIS IN CHILDREN: PROBLEMS AND PROSPECTS

2019 ◽  
Vol 20 (2) ◽  
pp. 99-107
Author(s):  
Galina I. Smirnova
Keyword(s):  
Atopic Dermatitis ◽  
Immune Responses ◽  
Proinflammatory Cytokines ◽  
Genetic Predisposition ◽  
Allergic Inflammation ◽  
Skin Lesions ◽  
Current Understanding ◽  
Epidermal Barrier ◽  
Barrier Integrity ◽  
Atopic Skin

There are presented modern data describing the current understanding of the pathogenesis of atopic dermatitis (AD): a genetic predisposition to atopy, disruptions of epidermal barrier integrity and a cascade of immune responses, contributing allergic inflammation in the skin. There are both described several mechanisms of acute and chronic phases of AD, the main directions of pathogenetically substantiated treatment of AD in children and indicated the prospects of new preparations specific blockers of proinflammatory cytokines involved in the development of AD - crisaborole, apremilast, dupilumab, lebrikizumab, tralokinumab, tezepelumab. There is especially presented in details external therapy of atopic skin lesions in children with the use of means of modern dermatological cosmetics.

scholarly journals Anti-Inflammatory Effect of Qingpeng Ointment in Atopic Dermatitis-Like Murine Model

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yun-Zhu Li ◽  
Xue-Yan Lu ◽  
Wei Jiang ◽  
Lin-Feng Li
Keyword(s):  
Atopic Dermatitis ◽  
Chinese Medicine ◽  
Mast Cells ◽  
Mrna Expression ◽  
Murine Model ◽  
Skin Lesions ◽  
Serum Immunoglobulin ◽  
Repeated Application ◽  
Immune Dysfunctions ◽  
Inflammatory Effect

Qingpeng ointment (QP) is a Chinese medicine which has been used in treatment of atopic dermatitis (AD) in China. AD-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene (DNFB) on shaved backs. The mice were then treated for 2 weeks with QP of different concentrations and Mometasone Furoate cream (MF), respectively. Macroscopic and microscopic changes of the skin lesions were observed after the treatment. The levels of serum immunoglobulin (Ig) E, tissue interferon (IFN)-γ, and interleukin (IL)-4 and IL-17A and the levels of involucrin, filaggrin, and kallikrein7 in epidermis were measured. The results show severe dermatitis with immune profiles similar to human acute AD. A significant infiltration of CD4+T and mast cells was observed in dermis of lesion but inhibited by QP after a 2-week treatment with it. The production of IgE, IL-4 and the mRNA expression of IL-17A were also suppressed, but the level of IFN-γwas increased. MF suppressed all production of these cytokines and IgE. Accordingly, the mechanism of QP on AD might correlate with its ability of modulating the immune dysfunctions rather than suppressing them. It had no effect on expressions of involucrin and filaggrin, except that its vehicle decreased the level of kallikrein7.

Inhibitory effect of 5,6-dihydroergosteol-glucoside on atopic dermatitis-like skin lesions via suppression of NF-κB and STAT activation.

2015 ◽  
Vol 79 (3) ◽  
pp. 252-261 ◽  
Author(s):  
Mira Jung ◽  
Tae Hoon Lee ◽  
Hyun Jeoung Oh ◽  
Hakwon Kim ◽  
Youngsook Son ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Skin Lesions ◽  
Inhibitory Effect

scholarly journals Inhibitory Effect ofNelumbo nucifera(Gaertn.) on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Rajendra Karki ◽  
Myung-A Jung ◽  
Keuk-Jun Kim ◽  
Dong-Wook Kim
Keyword(s):  
Atopic Dermatitis ◽  
Severity Score ◽  
Histopathological Examination ◽  
Skin Lesions ◽  
Transepidermal Water Loss ◽  
Inhibitory Effect ◽  
Clinical Severity ◽  
Inflammatory Skin Disease ◽  
Level Measurement ◽  
Different Doses

Atopic dermatitis (AD) is a chronic inflammatory skin disease which has a complex etiology that encompasses immunologic responses. The study was carried out to examine the effect ofNelumbo nucifera(Gaertn.) leaf (NL) on the AD-like skin lesion induced by repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) on the dorsal skin of NC/Nga mice. Three different doses of NL (5, 25, and 50 mg/mice/day) were administered orally from the day of sensitization with DNCB for 4 weeks. The efficacy of NL was judged by histopathological examination, blood IgE level, measurement of transepidermal water loss (TEWL), scratching behavior, and skin severity score. NL resulted in the suppression of clinical severity score, TEWL, scratching behavior, and blood IgE level. Histopathologic analyses revealed that thickening of the epidermis and mast cell degranulation was significantly reduced in NL group. These results suggest that NL may be a useful natural resource for the management of AD.

scholarly journals Improvement of Atopic Dermatitis-Like Skin Lesions by Platycodon grandiflorum Fermented by Lactobacillus plantarum in NC/Nga Mice

2012 ◽  
Vol 35 (8) ◽  
pp. 1222-1229 ◽  
Author(s):  
Min-Soo Kim ◽  
Wan-Gi Kim ◽  
Hwan-Suck Chung ◽  
Byoung-Woo Park ◽  
Kyoo-Seok Ahn ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Lactobacillus Plantarum ◽  
Skin Lesions ◽  
Platycodon Grandiflorum

scholarly journals Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hoyoung Lee ◽  
Jun Kyoung Lee ◽  
Hyekyung Ha ◽  
Mee-Young Lee ◽  
Chang-Seob Seo ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Skin Lesions ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Hacat Cells ◽  
Angelicae Dahuricae Radix

We examined whether Angelicae Dahuricae Radix (AR) suppresses the development of atopic dermatitis (AD)-like skin lesions induced byDermatophagoides farinaein NC/Nga mice. To investigate the effect of AR, we measured the AD severity score, measured plasma levels of IgE and histamine, and performed histological analysis in NC/Nga mice. We also confirmed the anti-inflammatory effects of AR by measuring TARC/CCL17 production from LPS-treated RAW 264.7 cells and mRNA levels of TARC and MDC/CCL22 in TNF-α/IFN-γ-treated HaCaT cells. 10 mg/day of AR extract was applied for 4 weeks to NC/Nga mice. Both the AR extract and 0.1% tacrolimus suppressed the development of AD-like skin lesions and reduced dermatitis scores of the back and ear skin. AR extracts caused an inhibition of histological changes induced by repeated application ofD. farinaeand a reduction of IgE and histamine levels in plasma (P<0.05). Furthermore, NO production in LPS-treated RAW 264.7 cells was diminished in a dose-dependent manner, and hTARC production and TARC and MDC mRNA levels in TNF-α/IFN-γ-treated HaCaT cells were diminished by AR. The inhibitory effect of AR on NO, TARC and MDC production may be associated with the suppression of AD-like skin lesions inD. farinae-induced NC/Nga mice.

Specific substance of Maruyama (SSM) suppresses immune responses in atopic dermatitis-like skin lesions in DS-Nh mice by modulating dendritic cell functions

2011 ◽  
Vol 63 (3) ◽  
pp. 184-190 ◽  
Author(s):  
Tsuyoshi Mitsuishi ◽  
Kenji Kabashima ◽  
Hideaki Tanizaki ◽  
Ikuroh Ohsawa ◽  
Fumino Oda ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Dendritic Cell ◽  
Immune Responses ◽  
Skin Lesions ◽  
Cell Functions ◽  
Specific Substance
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