Kidney, skeletal muscle and myocardium as potential target sites of Pygeum africanum toxicity in Wistar rats

2019 ◽  
Vol 17 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Natasa Duborija-Kovacevic ◽  
Zdenko Tomic
Keyword(s):  
Skeletal Muscle ◽  
Wistar Rats ◽  
Potential Target ◽  
Target Sites ◽  
Pygeum Africanum

scholarly journals Differences in the Role of HDACs 4 and 5 in the Modulation of Processes Regulating MAFbx and MuRF1 Expression during Muscle Unloading

2020 ◽  
Vol 21 (13) ◽  
pp. 4815 ◽  
Author(s):  
Ekaterina P. Mochalova ◽  
Svetlana P. Belova ◽  
Tatiana Y. Kostrominova ◽  
Boris S. Shenkman ◽  
Tatiana L. Nemirovskaya
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Histone Deacetylases ◽  
Wistar Rats ◽  
Hindlimb Suspension ◽  
Skeletal Muscle Atrophy ◽  
E3 Ligases ◽  
Male Wistar Rats ◽  
Muscle Unloading

Unloading leads to skeletal muscle atrophy via the upregulation of MuRF-1 and MAFbx E3-ligases expression. Reportedly, histone deacetylases (HDACs) 4 and 5 may regulate the expression of MuRF1 and MAFbx. To examine the HDAC-dependent mechanisms involved in the control of E3-ubiquitin ligases expression at the early stages of muscle unloading we used HDACs 4 and 5 inhibitor LMK-235 and HDAC 4 inhibitor Tasqinimod (Tq). Male Wistar rats were divided into four groups (eight rats per group): nontreated control (C), three days of unloading/hindlimb suspension (HS) and three days HS with HDACs inhibitor LMK-235 (HSLMK) or Tq (HSTq). Treatment with LMK-235 diminished unloading-induced of MAFbx, myogenin (MYOG), ubiquitin and calpain-1 mRNA expression (p < 0.05). Tq administration had no effect on the expression of E3-ligases. The mRNA expression of MuRF1 and MAFbx was significantly increased in both HS and HSTq groups (1.5 and 4.0 folds, respectively; p < 0.05) when compared with the C group. It is concluded that during three days of muscle unloading: (1) the HDACs 4 and 5 participate in the regulation of MAFbx expression as well as the expression of MYOG, ubiquitin and calpain-1; (2) the inhibition of HDAC 4 has no effect on MAFbx expression. Therefore, HDAC 5 is perhaps more important for the regulation of MAFbx expression than HDAC 4.

Burn Injury Induces Skeletal Muscle Degeneration, Inflammatory Host Response, and Oxidative Stress in Wistar Rats

2015 ◽  
Vol 36 (3) ◽  
pp. 428-433 ◽  
Author(s):  
Nathalia Trasmonte da Silva ◽  
Hananiah Tardivo Quintana ◽  
Jeferson André Bortolin ◽  
Daniel Araki Ribeiro ◽  
Flavia de Oliveira
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Host Response ◽  
Wistar Rats ◽  
Burn Injury ◽  
Muscle Degeneration ◽  
And Oxidative Stress

scholarly journals Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

2016 ◽  
Vol 34 (5) ◽  
pp. 187-193 ◽  
Author(s):  
Mayna Adabbo ◽  
Fernanda Rossi Paolillo ◽  
Paulo Sérgio Bossini ◽  
Natalia Camargo Rodrigues ◽  
Vanderlei Salvador Bagnato ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Laser Therapy ◽  
Wistar Rats ◽  
Treadmill Training ◽  
Low Level Laser Therapy ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

scholarly journals Changes in bioavailability and tissue distribution of zinc caused by magnesium deficiency in rats

1994 ◽  
Vol 72 (2) ◽  
pp. 315-323 ◽  
Author(s):  
Elena Planells ◽  
Pilar Aranda ◽  
Ana Lerma ◽  
Juan Llopis
Keyword(s):  
Skeletal Muscle ◽  
Tissue Distribution ◽  
Intestinal Absorption ◽  
Whole Blood ◽  
Wistar Rats ◽  
Magnesium Deficiency ◽  
Dry Weight ◽  
Deficient Diet ◽  
Mg Deficiency ◽  
Zn Concentration

The effect of a Mg-deficient diet (200 mg Mg/kg feed) on the bioavailability of dietary Zn and the concentration of this cation in plasma, whole blood, skeletal muscle, kidney, heart and brain of Wistar rats was studied after 7, 35, 42, 49, 56, 63 and 70 d. Mg deficiency significantly decreased Zn in whole blood on day 42 of the experiment, but there was no significant change in plasma Zn throughout the 70 d study period. The Mg-deficient diet significantly increased intestinal absorption of Zn, Zn balance, and Zn concentration in femur and kidney, but decreased Zn concentration in the heart despite the increase in dry weight of this organ. No change was found in brain Zn concentration.

scholarly journals Effect of Resistance Exercise and Vitamin C Intake on Expression of Telomerase Reverse Transcriptase and Telomere Repeat Binding Factor-2 Genes and the Diameter and Number of Myofibrils in Old Rats

2021 ◽  
Vol 10 (4) ◽  
pp. 396-409
Author(s):  
Yousef Shirkhani ◽  
◽  
Maghsoud Peeri ◽  
Mohammad Ali Azarbayjani ◽  
Hassan Matinhomaee ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin C ◽  
Resistance Exercise ◽  
Wistar Rats ◽  
Telomere Repeat ◽  
Vitamin Intake ◽  
Binding Factor ◽  
Male Wistar Rats ◽  
Old Rats ◽  
The Difference

Objective: Cell aging is one of the most important and fundamental step in cellular behavior and reduces muscle mass and myofibrils. This study aims to investigate the effect of resistance exercise along with vitamin C consumption on the expression of Telomerase Reverse Transcriptase (TERT) and Telomere Repeat Binding Factor-2 (TRF2) genes and the diameter and number of skeletal muscle myofibrils in old male Wistar rats. Methods: This is an experimental study using control groups. Twenty-five male Wistar rats (280-320 g and aged 24 weeks) were randomly divided into five groups of young control, old+resistance exercise, old+vitamin intake, old+resistance exercise+vitamin intake, and old control. In the supplementation groups, rats received liposomal vitamin C daily by gavage per body weight. Weight attached to the tail gradually increased during 8 weeks of exercise (weeks 1 and 2 by 50%; weeks 3 and 4 by 50%; weeks 5 and 6 by 75%; weeks 7 and 8 by 100% of total body weight). Exercises were performed at 3-5 sets of 8-12 repetitions, 3 or 4 days a week. The Quantitative polymerase chain reaction method was used to evaluate the expression of TERT and TRF2 genes in muscle tissue. One-way ANOVA was used to examine the difference between the groups and Tukey’s post hoc test was used to determine between which groups the difference was significant. Results: Aging significantly reduced the expression of TERT, TRF2 genes and the diameter and number of myofibrils in skeletal muscle of rats (P=0.001). Resistance exercise along with vitamin C intake had no significant effect on TERT and TRF2 expression (P≥0.05), but caused a significant increase in the diameter and number of myofibrils (P= 0.001). Conclusion: Eight weeks of resistance exercise along with vitamin C supplementation can significantly increase the number and diameter of skeletal muscle myofibrils in old rats.

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