Genetic variation of Aedes aegypti mosquitoes across Thailand based on nuclear DNA sequences

Abstract Aphis craccivora Koch (Hemiptera: Aphididae) or cowpea aphid is a polyphagous insect pest that feeds on a variety of leguminous plants. We determined the contribution of host-associated genetic differentiation on population structure using the sequence data generated from analysis of mitochondrial cytochrome b oxidase (Cytb) and nuclear elongation factor-1 alpha (EF1-alpha) of A. craccivora collected from cultivated yardlong bean [Vigna unguiculata (L.) Walp. ssp. sesquipedalis (L.) H. Ohashi.] (Fabales: Fabaceae) and winged bean [Psophocarpus tetragonolobus (L.) D.C.] (Fabales: Fabaceae). Phylogenetic and haplotype network analyses revealed no evidence of strong host plant or geographical clustering in both the mitochondrial and nuclear gene dataset. A moderate, low-magnitude genetic distance (FST) between host plants and geographical localities was found in this study. An analysis of molecular variance (AMOVA) revealed that host plant and geography do not influence the structure of genetic variation in A. craccivora populations. Genetic variation between host plants at a location and host plants among locations demonstrated no consistent result for population subdivision of A. craccivora. These results suggest that geographical location and host plants do not significantly influence the genetic structure of A. craccivora, and this might be due to their high reproductive (parthenogenesis) ability and high migration (airborne) between host plants and regions of the country.

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Molecular markers confirm the presence of the alien snowflake coral Carijoa riisei (Octocorallia: Alcyonacea) in Cabo Verde Islands (Central-East Atlantic).

10.21203/rs.3.rs-366544/v1 ◽

2021 ◽

Author(s):

Evandro Pires Lopes ◽

Rui Freitas ◽

Raquel Xavier ◽

João Deus Soares ◽

António M Santos

Keyword(s):

Genetic Variation ◽

Molecular Markers ◽

Dna Sequences ◽

Nuclear Dna ◽

East Atlantic ◽

Bottleneck Effect ◽

Cabo Verde ◽

Coastal Marine ◽

Carijoa Riisei ◽

Potential Origin

Abstract The snowflake coral Carijoa riisei is one of the most invasive and successfully adapting species in the coastal marine environment. This species can disperse through ballast waters or fouling on the hull of ships, which allows it to expand very quickly to areas where it is not native. Recently C. riisei was identified in the Porto Grande Bay in São Vicente island in Cabo Verde Islands. We analysed mitochondrial and nuclear DNA sequences from specimens collected at this locality to determine the potential origin of this invasion. Lack of genetic variation in both mitochondrial and nuclear genes suggest a very recent invasion (bottleneck effect) but did not permit to establish its origin with total confidence.

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Genetic differentiation of Southeast Asian Paragonimus Braun, 1899 (Digenea: Paragonimidae) and genetic variation in the Paragonimus heterotremus complex examined by nuclear DNA sequences

Infection Genetics and Evolution ◽

10.1016/j.meegid.2021.104761 ◽

2021 ◽

Vol 90 ◽

pp. 104761

Author(s):

Chairat Tantrawatpan ◽

Sumonta Tapdara ◽

Takeshi Agatsuma ◽

Oranuch Sanpool ◽

Pewpan M. Intapan ◽

...

Keyword(s):

Genetic Variation ◽

Genetic Differentiation ◽

Dna Sequences ◽

Nuclear Dna ◽

Southeast Asian

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Genetic variation within Saurodactylus and its phylogenetic relationships within the Gekkonoidea estimated from mitochondrial and nuclear DNA sequences

Amphibia-Reptilia ◽

10.1163/156853808783431406 ◽

2008 ◽

Vol 29 (1) ◽

pp. 25-34 ◽

Cited By ~ 9

Author(s):

David James Harris ◽

Catarina Rato

Keyword(s):

Genetic Variation ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

Nuclear Dna ◽

Taxonomic Position ◽

Gene Region ◽

12S Rrna ◽

Nuclear Marker ◽

Nuclear Markers ◽

Mtdna Variability

Abstract Phylogenetic relationships of the three morphological forms within the gecko genus Saurodactylus were estimated using mtDNA (12S rRNA and ND4) sequences. High between morphological forms variation (up to 25% with ND4), confirms that all three deserve specific status. Saurodactylus mauritanicus and Saurodactylus brosseti are strongly supported as sister taxa. Our results again highlight the extremely high mtDNA variability almost universally reported from within gecko species. The position of Saurodactylus within the Gekkonoidea was also investigated. Although considered as a member of the sphaerodactyl geckos, its taxonomic position is still highly uncertain. Evaluation of C-mos nuclear DNA sequences supports many of the recent taxonomic rearrangements within the Gekkonoidea. Using this marker, Saurodactylus is paraphyletic, with S. mauritanicus and S. brosseti sister taxa to Teratoscincus przewalskii rather than Saurodactylus fasciatus. This is supported by a further nuclear marker, RAG1, although for this gene region sampling is more limited. Based on this paraphyly, supported by two independent nuclear markers, we suggest it likely that Saurodactylus will need to be partitioned into two genera, pending further investigations.

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Detection of Flaviviral-Like DNA Sequences in Aedes aegypti (Diptera: Culicidae) Collected From Argentina

Journal of Medical Entomology ◽

10.1093/jme/tjab073 ◽

2021 ◽

Author(s):

Melisa B Bonica ◽

Dario E Balcazar ◽

Ailen Chuchuy ◽

Jorge A Barneche ◽

Carolina Torres ◽

...

Keyword(s):

Public Health ◽

Aedes Aegypti ◽

Dna Sequences ◽

Immature Stages ◽

Public Health Burden ◽

The Past ◽

Health Burden ◽

The World ◽

Aegypti Genome ◽

Dengue Epidemics

Abstract Diseases caused by flaviviruses are a major public health burden across the world. In the past decades, South America has suffered dengue epidemics, the re-emergence of yellow fever and St. Louis encephalitis viruses, and the introduction of West Nile and Zika viruses. Many insect-specific flaviviruses (ISFs) that cannot replicate in vertebrate cells have recently been described. In this study, we analyzed field-collected mosquito samples from six different ecoregions of Argentina to detect flaviviruses. We did not find any RNA belonging to pathogenic flaviviruses or ISFs in adults or immature stages. However, flaviviral-like DNA similar to flavivirus NS5 region was detected in 83–100% of Aedes aegypti (L.). Despite being previously described as an ancient element in the Ae. aegypti genome, the flaviviral-like DNA sequence was not detected in all Ae. aegypti samples and sequences obtained did not form a monophyletic group, possibly reflecting the genetic diversity of mosquito populations in Argentina.

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