Deciphering bacteria associated with a pre-parasitic stage of the root-knot nematode Meloidogyne hapla in nemato-suppressive and nemato-conducive soils

The northern root-knot nematode, Meloidogyne hapla, is a major pest of many crop species. The objective of the study was to determine how M. hapla population dynamics is affected by two precrops, i.e., Trifolium pratense and Medicago sativa, in three crop durations: one, two and three years of continuous cultivation. Moreover, we set ourselves the task of evaluating the effect of the legume precrop soil on the growth of the succeeding tomato plant (Solanum lycopersicum) and on the nematode population. The experiment was performed outdoors in pots with naturally infected soil. Both precrop species investigated were found to modify the J2 nematode population density in the soil. The galls and nematode females with egg masses were observed on the roots of both studied plant species at the end of each growing season. They appeared to be more abundant on the red clover roots than on those of the alfalfa. The obtained data indicate that the spring soil sampling is more appropriate for the estimation of the M. hapla population density in the red clover precrop soil. The legume precrop soil had a limiting effect on tomato growth and fruit yield. The nematode population negatively influenced tomato growth. The experiment revealed that tomato plants could be planted in alfalfa precrop soil following at least three years of continuous alfalfa cultivation. The same cannot be said of the cultivation of red clover as a precrop for tomatoes.

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Interaction of Meloidogyne hapla and Verticillium dahliae, and the chemical control of wilt in strawberry

Australian Journal of Experimental Agriculture ◽

10.1071/ea9700493 ◽

1970 ◽

Vol 10 (45) ◽

pp. 493 ◽

Cited By ~ 2

Author(s):

JW Meagher ◽

PT Jenkins

Keyword(s):

Broad Spectrum ◽

Verticillium Dahliae ◽

Methyl Bromide ◽

Chemical Control ◽

Soil Fumigation ◽

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Methyl Isothiocyanate ◽

Severity Of Disease ◽

Verticillium Dahliae Kleb

In a field experiment with strawberries, pre-plant treatments with broad-spectrum fumigants methyl bromide-chloropicrin (450 kg/ha) or methyl isothiocyanate-dichloropropene (500 l/ha) (and 300 l/ha) controlled wilt caused by Verticillium dahliae Kleb and resulted in increased yields. Soil fumigation with the nematicide ethylene dibromidz (105 l/ha) also improved yields. It controlled the root-knot nematode (Meloidogyne hapla Chitwood), delayed the onset of wilt symptoms and reduced the severity of disease. This indicated a nematode-fungus interaction and is the first report of a Meloidogyne-Verticillium interaction in strawberry.

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Using plant essences as alternative mean for northern root-knot nematode (Meloidogyne hapla) management

Journal of Pest Science ◽

10.1007/s10340-010-0287-4 ◽

2010 ◽

Vol 83 (3) ◽

pp. 217-221 ◽

Cited By ~ 17

Author(s):

Ondřej Douda ◽

Miloslav Zouhar ◽

Jana Mazáková ◽

Eva Nováková ◽

Roman Pavela

Keyword(s):

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Plant Essences

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First Report of the Root-Knot Nematode Meloidogyne hapla Parasitizing Roses in Ethiopia

Plant Disease ◽

10.1094/pdis-04-14-0383-pdn ◽

2014 ◽

Vol 98 (9) ◽

pp. 1286-1286 ◽

Cited By ~ 5

Author(s):

Beira H. Meressa ◽

H. Heuer ◽

H.-W. Dehne ◽

J. Hallmann

Keyword(s):

Addis Ababa ◽

Sequence Similarity ◽

Infected Plant ◽

Export Market ◽

Economic Losses ◽

Parasitic Nematodes ◽

Morphological Identification ◽

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Damage Potential

Meloidogyne hapla is one of the most damaging plant-parasitic nematodes in temperate regions. This nematode has a wide host range with more than 500 plant taxa including roses. In Ethiopia, rose production has developed over the past 10 years to the second most important export market after coffee. Considering the high damage potential of M. hapla, infestation of roses in Ethiopia with this nematode could result in major economic losses. Therefore, awareness of this nematode species is extremely important. During two surveys conducted in August 2011 and April 2012, M. hapla was detected in soil samples from six out of nine rose producing farms located in the districts of Ziway, Holleta, Sebeta, and Menagesha. At infested farms, rose plants appeared stunted and less productive and often showed symptoms of chlorosis and wilting. Identification was based on morphological and morphometrical characters of females, males, and second-stage juveniles, which were all within the range of variability known for this species (4). Shape of juvenile stylet knobs, shape of male head, and perennial pattern of the females with characteristic punctuations between the anus and tail terminus were also typical for M. hapla. The morphological identification was confirmed by sequence analysis of the D2-D3 expansion segment of the 28S rDNA gene following amplification with the primers D2A (5′-ACAAGTACCGTGAGGGAAAGTT-3′) and D3B (5′-TCGGAAGGAACCAGCTACTA-3′) (1). PCR products were purified and sequenced at the Macrogene sequencing facility service (Amsterdam, The Netherlands). Sequences were deposited in GenBank (KJ645427 to 33). The sequences were compared with previously published sequences in NCBI database and showed 96 to 100% sequence similarity with M. hapla accession nos. GQ130139, DQ328685, KF430798, and DQ145641. Unfortunately, comparison of sequences did not provide further information about the origin of this Ethiopian population, if it is native to Ethiopia or was imported with infected plant material. To the best of our knowledge, this is the first record of M. hapla occurring in Ethiopia. M. hapla is known as a serious pest of roses in colder climate regions. In Africa, it was previously reported from Tanzania (3) and South Africa (2). Thus, it appears that this species has now become also established in Ethiopia at higher altitudes (1,400 to 2,100 m above sea level) within the urban hinterland of Addis Ababa. References: (1) Baldwin et al. Mol. Phy. Evol. 8:248, 1887. (2) J. H. O'Bannon. Institute Agri. Res. 29, 1975. (3) E. Onkendi and L. N. Moleleki. Eur. J. Pl. Pathol. 136:1, 2013. (4) A. G. Whitehead. Trans. Zool. Soc. Lon.31:263, 1968.

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Liquid bioformulations for the management of root-knot nematode, Meloidogyne hapla that infects carrot

Crop Protection ◽

10.1016/j.cropro.2018.08.022 ◽

2018 ◽

Vol 114 ◽

pp. 155-161 ◽

Cited By ~ 4

Author(s):

Seenivasan Nagachandrabose

Keyword(s):

Meloidogyne Hapla ◽

Root Knot Nematode

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Irrigation and Rootstocks to Manage Northern Root-Knot Nematode During Wine Grape Vineyard Establishment

Plant Health Progress ◽

10.1094/php-06-21-0097-rs ◽

2021 ◽

Author(s):

Katherine East ◽

Inga Zasada ◽

R. Paul Schreiner ◽

Michelle Marie Moyer

Keyword(s):

Late Summer ◽

Field Trials ◽

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Cultural Management ◽

Host Status ◽

Management Approaches ◽

Eastern Washington ◽

Establishment Phase

Vineyard replanting in Washington state can be negatively impacted by the plant-parasitic nematode Meloidogyne hapla. Chemically-focused nematode management programs do not offer long-term suppression, however, this may be achieved through the adoption of cultural approaches such as rootstocks and irrigation. Nematode-resistant rootstocks are used extensively in other regions, but many have not been tested against M. hapla. Vineyards in eastern Washington are irrigated, so manipulating available soil water may also impact nematode development. In 2017, two field trials were established in eastern Washington to evaluate the effects of: 1) late-summer water limitation on M. hapla population development, and 2) host status of 1103 Paulsen, 3309 Couderc and Matador rootstocks for M. hapla. The efficacy of these cultural management approaches was evaluated under three initial M. hapla densities (0, 50, and 250 M. hapla J2 per 250 g soil) in both trials. Reducing irrigation to manage M. hapla infestation of grape roots was ineffective and may cause harm to the vines by inducing too much water stress. Conversely, rootstocks effectively reduced population densities of M. hapla. Overall, rootstocks show the most promise as a cultural tool to manage M. hapla during the establishment phase in Washington vineyards.

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Rapid and visual detection of Meloidogyne hapla using recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay

Plant Disease ◽

10.1094/pdis-06-20-1345-re ◽

2020 ◽

Author(s):

Zhiqiang Song ◽

Xiai Yang ◽

Xiaowei Zhang ◽

Mingbao Luan ◽

Bing Guo ◽

...

Keyword(s):

Rapid Detection ◽

Lateral Flow ◽

Recombinase Polymerase Amplification ◽

Economic Losses ◽

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Resource Limited ◽

Lateral Flow Dipstick ◽

Wide Range ◽

And Control

The northern root-knot nematode, Meloidogyne hapla, is a biotrophic parasite that infects many crops and causes severe economic losses worldwide. Rapid and accurate detection of M. hapla is crucial for disease forecasting and control. We developed a recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay for rapid detection of M. hapla. The primers and a probe were designed based on the effector gene 16D10 sequence and were highly specific to M. hapla. The RPA reaction was performed at a wide range of temperatures from 25 to 45°C within 5 to 25 min, and the amplicon was visualized directly on the LFD within 5 min. The detection limits of the RPA-LFD assay were 10-3 female and 10-2 J2/0.5 g of soil, which was 10 times more sensitive than the conventional PCR assay. In addition, the RPA-LFD assay can detect M. hapla from infested plant roots and soil samples, and the entire detection process can be completed within 1.5 h. These results indicate that the RPA-LFD assay is a simple, rapid, specific, sensitive, and visual method that can be used for rapid detection of M. hapla in the field and in resource-limited conditions.

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The diverse nematicidal properties and biocontrol efficacy of Bacillus thuringiensis Cry6A against the root-knot nematode Meloidogyne hapla

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2014.12.011 ◽

2015 ◽

Vol 125 ◽

pp. 73-80 ◽

Cited By ~ 26

Author(s):

Ziquan Yu ◽

Jing Xiong ◽

Qiaoni Zhou ◽

Haiyan Luo ◽

Shengbiao Hu ◽

...

Keyword(s):

Bacillus Thuringiensis ◽

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Biocontrol Efficacy

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Effects of DCPA on Seedling Infection by Root-Knot Nematode

Weed Science ◽

10.1017/s004317450003650x ◽

1974 ◽

Vol 22 (1) ◽

pp. 51-54 ◽

Cited By ~ 4

Author(s):

R. Kent Romney ◽

J. Lamar Anderson ◽

Gerald D. Griffin

Keyword(s):

Root Exudate ◽

Cell Structure ◽

Meloidogyne Hapla ◽

Root Knot Nematode ◽

Fibrous Root ◽

Untreated Soil ◽

Nematode Larvae ◽

Soil Adherence ◽

Altered Cell ◽

Seedling Infection

Onion (Allium cepaL.) and bean (Phaseolus vulgarisL.) seedlings grown in soil treated with DCPA (dimethyl tetrachloroterephthalate) were less susceptible to northern root-knot nematode (Meloidogyne haplaChitwood) infection than seedlings grown in untreated soil. DCPA treatment of nematode larvae did not alter their pathogenicity. Resistance could be correlated with altered cell structure of the root epidermis, increased root exudate as evidenced by soil adherence, reduced fibrous root development, or both.

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