Soybean glycinin caused NADPH-oxidase-regulated ROS overproduction and decreased ROS elimination capacity in the mid and distal intestine of juvenile grass carp (Ctenopharyngodon idella)

Aquaculture ◽  
2020 ◽  
Vol 516 ◽  
pp. 734651 ◽  
Author(s):  
Ya-lin Zhang ◽  
Wei-Dan Jiang ◽  
Xu-dong Duan ◽  
Lin Feng ◽  
Pei Wu ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Grass Carp ◽  
Elimination Capacity ◽  
Ctenopharyngodon Idella ◽  
Distal Intestine ◽  
Grass Carp Ctenopharyngodon Idella

scholarly journals Effects of Dietary Zearalenone on Oxidative Stress, Cell Apoptosis, and Tight Junction in the Intestine of Juvenile Grass Carp (Ctenopharyngodon idella)

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 333 ◽  
Author(s):  
Ya-Li Wang ◽  
Xiao-Qiu Zhou ◽  
Wei-Dan Jiang ◽  
Pei Wu ◽  
Yang Liu ◽  
...  
Keyword(s):  
Tight Junctions ◽  
Grass Carp ◽  
Structural Integrity ◽  
Ctenopharyngodon Idella ◽  
Related Factor ◽  
Distal Intestine ◽  
Fish Intestine ◽  
Mitogen Activated Protein ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Proximal Intestine

Zearalenone (ZEA) is a prevalent mycotoxin with high toxicity in animals. In order to study its effect on juvenile grass carp (Ctenopharyngodon idella), six diets supplemented with different levels of ZEA (0, 535, 1041, 1548, 2002, and 2507 μg/kg diet) for 10 weeks were studied to assess its toxicity on intestinal structural integrity and potential mechanisms of action. Our report firstly proved that ZEA led to growth retardation and body deformity, and impaired the intestinal structural integrity of juvenile grass carp, as revealed by the following findings: (1) ZEA accumulated in the intestine and caused histopathological lesions; (2) ZEA resulted in oxidative injury, apoptosis, and breached tight junctions in the fish intestine, which were probably associated with Nuclear factor-erythroid 2-related factor 2 (Nrf2), p38 mitogen activated protein kinases (p38MAPK), and myosin light chain kinase (MLCK) signaling pathways, respectively. ZEA had no influence on the antioxidant gene levels of Kelch-like ECH associating protein 1 (Keap1)b (rather than Keap1a), glutathione-S-transferase (GST)P1, GSTP2 (not in the distal intestine (DI)), tight junctions occludin, claudin-c (not in the proximal intestine (PI)), or claudin-3c (not in the mid intestine (MI) or DI).

scholarly journals Threonine deficiency decreased intestinal immunity and aggravated inflammation associated withNF-κBandtarget of rapamycinsignalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection withAeromonas hydrophila

2017 ◽  
Vol 118 (2) ◽  
pp. 92-108 ◽  
Author(s):  
Yu-Wen Dong ◽  
Wei-Dan Jiang ◽  
Yang Liu ◽  
Pei Wu ◽  
Jun Jiang ◽  
...  
Keyword(s):  
G Protein ◽  
Grass Carp ◽  
Transforming Growth Factor ◽  
Ctenopharyngodon Idella ◽  
Mrna Levels ◽  
Intestinal Immunity ◽  
Distal Intestine ◽  
Tnf Α ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Proximal Intestine

AbstractThis study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six iso-nitrogenous semi-purified diets containing graded levels of threonine (3·99–21·66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged withAeromonas hydrophilafor 14 d. Results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozyme activities (except in the distal intestine), acid phosphatase activities, complement 3 (C3) and C4 contents and IgM contents (except in the proximal intestine (PI)), and it down-regulated the transcript abundances ofliver-expressed antimicrobial peptide(LEAP)-2A,LEAP-2B,hepcidin, IgZ, IgMandβ-defensin1(except in the PI) (P<0·05); (2) could up-regulate intestinal pro-inflammatory cytokinesTNF-α,IL-1β,IL-6, IL-8andIL-17DmRNA levels partly related toNF-κBsignalling; (3) could down-regulate intestinal anti-inflammatory cytokinetransforming growth factor(TGF)-β1,TGF-β2,IL-4/13A(notIL-4/13B) andIL-10mRNA levels partly by target of rapamycin signalling. Finally, on the basis of the specific growth rate, against the enteritis morbidity and IgM contents, the optimum threonine requirements were estimated to be 14·53 g threonine/kg diet (4·48 g threonine/100 g protein), 15.05 g threonine/kg diet (4·64 g threonine/100 g protein) and 15·17 g threonine/kg diet (4·68 g threonine/100 g protein), respectively.

scholarly journals Oral Vaccination of Grass Carp (Ctenopharyngodon idella) with Baculovirus-Expressed Grass Carp Reovirus (GCRV) Proteins Induces Protective Immunity against GCRV Infection

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 41
Author(s):  
Changyong Mu ◽  
Qiwang Zhong ◽  
Yan Meng ◽  
Yong Zhou ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Grass Carp ◽  
Oral Vaccine ◽  
Serum Antibody ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Oral Vaccination ◽  
Grass Carp Reovirus ◽  
Silkworm Pupae ◽  
Grass Carp Ctenopharyngodon Idella

The grass carp reovirus (GCRV) causes severe hemorrhagic disease with high mortality and leads to serious economic losses in the grass carp (Ctenopharyngodon idella) industry in China. Oral vaccine has been proven to be an effective method to provide protection against fish viruses. In this study, a recombinant baculovirus BmNPV-VP35-VP4 was generated to express VP35 and VP4 proteins from GCRV type Ⅱ via Bac-to-Bac baculovirus expression system. The expression of recombinant VP35-VP4 protein (rVP35-VP4) in Bombyx mori embryo cells (BmE) and silkworm pupae was confirmed by Western blotting and immunofluorescence assay (IFA) after infection with BmNPV-VP35-VP4. To vaccinate the grass carp by oral route, the silkworm pupae expressing the rVP35-VP4 proteins were converted into a powder after freeze-drying, added to artificial feed at 5% and fed to grass carp (18 ± 1.5 g) for six weeks, and the immune response and protective efficacy in grass carp after oral vaccination trial was thoroughly investigated. This included blood cell counting and classification, serum antibody titer detection, immune-related gene expression and the relative percent survival rate in immunized grass carp. The results of blood cell counts show that the number of white blood cells in the peripheral blood of immunized grass carp increased significantly from 14 to 28 days post-immunization (dpi). The differential leukocyte count of neutrophils and monocytes were significantly higher than those in the control group at 14 dpi. Additionally, the number of lymphocytes increased significantly and reached a peak at 28 dpi. The serum antibody levels were significantly increased at Day 14 and continued until 42 days post-vaccination. The mRNA expression levels of immune-related genes (IFN-1, TLR22, IL-1β, MHC I, Mx and IgM) were significantly upregulated in liver, spleen, kidney and hindgut after immunization. Four weeks post-immunization, fish were challenged with virulent GCRV by intraperitoneal injection. The results of this challenge study show that orally immunized group exhibited a survival rate of 60% and relative percent survival (RPS) of 56%, whereas the control group had a survival rate of 13% and RPS of 4%. Taken together, our results demonstrate that the silkworm pupae powder containing baculovirus-expressed VP35-VP4 proteins could induce both non-specific and specific immune responses and protect grass carp against GCRV infection, suggesting it could be used as an oral vaccine.

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