Sub-cellular partitioning of metals (Cd, Cu, Zn) in the gills of a freshwater bivalve, Pyganodon grandis: role of calcium concretions in metal sequestration

2005 ◽  
Vol 71 (4) ◽  
pp. 319-334 ◽  
Author(s):  
Emmanuelle Bonneris ◽  
Anik Giguère ◽  
Olivier Perceval ◽  
Thierry Buronfosse ◽  
Stéphane Masson ◽  
...  
Keyword(s):  
Freshwater Bivalve ◽  
Metal Sequestration ◽  
Pyganodon Grandis

Field transplantation of a freshwater bivalve, Pyganodon grandis, across a metal contamination gradient. I. Temporal changes in metallothionein and metal (Cd, Cu, and Zn) concentrations in soft tissues

1995 ◽  
Vol 52 (4) ◽  
pp. 690-702 ◽  
Author(s):  
Y. Couillard ◽  
P. G. C. Campbell ◽  
A. Tessier ◽  
J. C. Auclair ◽  
J. Pellerin-Massicotte
Keyword(s):  
Soft Tissues ◽  
Shell Growth ◽  
Mining Area ◽  
Metal Exposure ◽  
Steady Increase ◽  
Freshwater Bivalve ◽  
Slow Increase ◽  
Metal Levels ◽  
Pyganodon Grandis ◽  
Over Time

To test the response of the freshwater bivalve Pyganodon grandis (formerly Anodonta grandis) to increased metal exposure in the field, we transferred specimens (8 cm length; 4–6 years old) from a less to a more contaminated lake in the mining area of Rouyn-Noranda, in northwestern Québec. The transplanted bivalves were maintained in open enclosures placed in the bottom sediments of the contaminated lake. Up to 16 individuals were removed from pairs of enclosures at times t = 0 (June 1990), 5, 14, 30, 60, 90, and 400 d; tissue concentrations of metallothionein (MT) and metals were monitored over time. Measurements on control molluscs enclosed in their lake of origin showed that enclosure per se had no apparent effect on tissue [MT] or tissue metal levels, but did decrease shell growth. Metallothionein levels in specimens transplanted to the more contaminated lake showed a slow but steady increase with time; in contrast, MT levels in the control populations showed only modest seasonal fluctuations. The increase in MT over time in the transplanted bivalves was closely correlated with a similar slow increase in soft tissue [Cd]. We conclude that MT in the freshwater bivalve P. grandis is a promising biochemical indicator of metal exposure.

Modeling cadmium uptake from water and food by the freshwater bivalve Pyganodon grandis

2010 ◽  
Vol 67 (11) ◽  
pp. 1874-1888 ◽  
Author(s):  
Sophie Cooper ◽  
Landis Hare ◽  
Peter G.C. Campbell
Keyword(s):  
Digestive Gland ◽  
Dietary Exposure ◽  
Field Studies ◽  
Temporal Variations ◽  
Pseudokirchneriella Subcapitata ◽  
Relative Importance ◽  
Freshwater Bivalve ◽  
Cd Accumulation ◽  
Pyganodon Grandis ◽  
Biodynamic Model

For this study, the unionid bivalve Pyganodon grandis was either exposed to dissolved cadmium (Cd) or fed Cd-contaminated algae ( Pseudokirchneriella subcapitata ) during short-term laboratory experiments. Cadmium accumulated largely in the digestive gland after a dietary exposure, or in the gills after an aqueous exposure; in these latter experiments, Cd accumulation from the dissolved phase increased as bivalve filtration rates increased. The results of these uptake experiments were used to parameterize a biodynamic model, which was then used to estimate the relative importance of water and food as sources of Cd for this bivalve, and to predict steady state Cd concentrations in the gills and digestive gland of native bivalves. In comparisons between the simulations and data obtained from earlier field studies on P. grandis, the model adequately predicted Cd concentrations in P. grandis gills, except in Ca-rich lakes, whereas it tended to overestimate Cd concentrations in the digestive gland. The field simulations indicate that water is the main source of Cd for both the gills (relative importance water:food::99:1) and the digestive gland (water:food::80:20). These results will facilitate the interpretation of spatial and temporal variations in Cd concentrations in free-living P. grandis, which is a promising metal biomonitor.

Field transplantation of a freshwater bivalve, Pyganodon grandis, across a metal contamination gradient. II. Metallothionein response to Cd and Zn exposure, evidence for cytotoxicity, and links to effects at higher levels of biological organization

1995 ◽  
Vol 52 (4) ◽  
pp. 703-715 ◽  
Author(s):  
Y. Couillard ◽  
P. G. C. Campbell ◽  
J. G. Auclair ◽  
J. Pellerin-Massicotte
Keyword(s):  
Gill Tissue ◽  
Weight Fraction ◽  
Condition Index ◽  
Mining Area ◽  
Low Molecular Weight ◽  
Biological Organization ◽  
Freshwater Bivalve ◽  
Cellular Toxicity ◽  
Pyganodon Grandis ◽  
Anodonta Grandis

To examine links between the metallothionein (MT) status of an organism and its general health, we transplanted adult specimens of the freshwater bivalve Pyganodon grandis (formerly Anodonta grandis) from a less to a more contaminated lake in the mining area of Rouyn-Noranda, in northwestern Québec. The transplanted bivalves were maintained in open enclosures placed in the bottom sediments of the contaminated lake; in addition, indigenous specimens were maintained in control enclosures in their lake of origin. Up to 16 individuals were removed from pairs of enclosures at times t = 0 (June 1990), 5, 14, 30, 60, 90, and 400 d. Excised gill tissue was analyzed for metallothionein, Cd, Cu, Zn, Ca, and malondialdehyde (MDA), a product of lipid peroxidation. Metal partitioning in the gill cytosol, as determined on a subset of gill samples from transplanted molluscs, changed markedly during the experiment. After 400 d, Cd was present in the low molecular weight fraction of the gill cytosol, and symptoms of cellular toxicity were detected in the transplanted molluscs (elevated [MDA] and [Ca]). At the whole organism level, the marked transplanted bivalves grew more slowly over the 400-d experiment than did marked control bivalves in Lake Opasatica, and their condition index deteriorated over time.

Changes in subcellular metal partitioning in the gills of freshwater bivalves (Pyganodon grandis) living along an environmental cadmium gradient

1999 ◽  
Vol 56 (5) ◽  
pp. 774-784 ◽  
Author(s):  
Dacheng Wang ◽  
Yves Couillard ◽  
G C Campbell ◽  
Pierre Jolicoeur
Keyword(s):  
Mining Area ◽  
Size Exclusion ◽  
Metal Exposure ◽  
Relative Molecular Mass ◽  
Biological Organization ◽  
Freshwater Bivalve ◽  
Exclusion Chromatography ◽  
Saturation Method ◽  
Pyganodon Grandis ◽  
Different Levels

To test the response of the freshwater bivalve Pyganodon grandis to increased metal exposure in the field, specimens were collected from 10 lakes located along a known metal gradient in a mining area in northwestern Québec. Total gill concentrations of metallothionein (MT) were determined by the 203Hg saturation method for molluscs from each lake, and the distribution of Cd among various cytosolic ligands, including MT, was determined by size-exclusion chromatography. Gill MT concentrations responded to environmental exposure to Cd but not to Cu or Zn exposure; these spatial variations along the Cd gradient were more important than the seasonal summer variations in gill MT concentrations. Bivalves exposed to concentrations of dissolved free Cd2+ higher than ~1 nM in the external medium exhibited a marked increase of Cd in the low relative molecular mass ligand pool. Symptoms of toxic effects at different levels of biological organization were associated with this biochemical anomaly.

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