Validation of a multiplex qPCR assay for detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythia in subgingival plaque samples. A comparison with anaerobic culture

2019 ◽  
Vol 102 ◽  
pp. 199-204 ◽  
Author(s):  
Mª José Marín ◽  
Nagore Ambrosio ◽  
Ana O’Connor ◽  
David Herrera ◽  
Mariano Sanz ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Qpcr Assay ◽  
Subgingival Plaque ◽  
Anaerobic Culture ◽  
Tannerella Forsythia ◽  
Multiplex Qpcr ◽  
Detection And Quantification

scholarly journals PERIODONTOPHATOGENIC BACTERIA OF THE MAIN FACTORS OF EMERGENCE AND DEVELOPMENT OF PERIODONTITIS

2017 ◽  
pp. 101-112 ◽  
Author(s):  
V. N. Tsarev ◽  
E. N. Nikolaeva ◽  
E. V. Ippolitov
Keyword(s):  
Porphyromonas Gingivalis ◽  
Quantitative Evaluation ◽  
Inflammatory Diseases ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Actinobacillus Actinomycetemcomitans ◽  
Tannerella Forsythia ◽  
Main Factors ◽  
Level Of Importance ◽  
Development Risk

Presents a modern understanding of the role of periodontophatogenic bacteria types: Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Tannerella forsythia (Bacteroides forsythus) and Porphyromonas gingivalis as indicators of periodontitis emergence and development risk. Therminological definitions are given of factors, indicators and markers of risk of periodontium inflammatory diseases development that show the level of importance of their association with the disease as well as the quantitative evaluation of these associations.

scholarly journals Relative quantification of Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Aggregatibacter actinomycetemcomitans high-risk bacterial species in Romanian patients evaluated for periodontal disease

2017 ◽  
Author(s):  
Cosac Ion Constantin ◽  
Ionica Consuel ◽  
Ratiu Attila Cristian ◽  
Savu Lorand
Keyword(s):  
Periodontal Disease ◽  
Porphyromonas Gingivalis ◽  
Gingival Crevicular Fluid ◽  
Bacterial Species ◽  
Bacterial Load ◽  
Clinical Signs ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Treponema Denticola ◽  
Tannerella Forsythia ◽  
Complex Species

AbstractThree bacterial species pertaining to the red complex (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) and Aggregatibacter actinomycetemcomitans were investigated in relation to the incidence and severity of periodontal disease. A total of 259 patients were included in this study, 179 being diagnosed with periodontal disease. The gingival crevicular fluid samples were obtained from periodontal pockets and the presence and levels of target bacteria were assessed following DNA extraction and real-time quantitative PCR. Our results account for significant positive associations between the number of bacterial species from the red complex coexisting within a patient and several clinical signs (gingival bleeding, inflammation and bone deterioration). A similar positive association was found between bacterial load of the red complex species and the clinical Case Type diagnostic of the periodontal disease, as well as the probing depth with the most evident results for T. denticola. In conclusion, our study, a first for the Romanian population, confirms previous results found elsewhere and finds a possible regional pathogenic specificity for T. denticola as a major factor for periodontitis severity.

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