Study of lipid spectrum indicators and markers of vascular inflammation in patients with stable stenocardia and diabetes mellitus

Objective: Hyperhomocysteinemia (HHcy) is a potent risk factor for diabetic cardiovascular diseases. We have previously reported that hyperhomocysteinemia potentiates type 1 diabetes mellitus-induced inflammatory monocyte differentiation, vascular dysfunction, and atherosclerosis. However, the effects of hyperhomocysteinemia on vascular inflammation in type 2 diabetes mellitus (T2DM) and the underlying mechanism are unknown. Approach and Results: Here, we demonstrate that hyperhomocysteinemia was induced by a high methionine diet in control mice (homocysteine 129 µmol/L), which was further worsened in T2DM db/db mice (homocysteine 180 µmol/L) with aggravated insulin intolerance. Hyperhomocysteinemia potentiated T2DM-induced mononuclear cell, monocyte, inflammatory monocyte (CD11b + Ly6C + ), and M1 macrophage differentiation in periphery and aorta, which were rescued by folic acid-based homocysteine-lowering therapy. Moreover, hyperhomocysteinemia exacerbated T2DM-impaired endothelial-dependent aortic relaxation to acetylcholine. Finally, transfusion of bone marrow cells depleted for Ly6C by Ly6c shRNA transduction improved insulin intolerance and endothelial-dependent aortic relaxation in hyperhomocysteinemia+T2DM mice. Conclusions: Hyperhomocysteinemia potentiated systemic and vessel wall inflammation and vascular dysfunction partially via inflammatory monocyte subset induction in T2DM. Inflammatory monocyte may be a novel therapeutic target for insulin resistance, inflammation, and cardiovascular complications in hyperhomocysteinemia+T2DM.

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Determinants of Increased Serum Calprotectin in Patients with Type 2 Diabetes Mellitus

International Journal of Molecular Sciences ◽

10.3390/ijms21218075 ◽

2020 ◽

Vol 21 (21) ◽

pp. 8075

Author(s):

Milou M. Oosterwijk ◽

Stephan J.L. Bakker ◽

Tom Nilsen ◽

Gerjan Navis ◽

Gozewijn D. Laverman

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Vascular Inflammation ◽

Strong Association ◽

Median Serum ◽

Potential Biomarker ◽

Calprotectin Level ◽

Peripheral Artery Diseases

Circulating calprotectin is a potential biomarker for endovascular inflammation in type 2 diabetes mellitus (T2DM). We investigated the determinants of calprotectin and its relationship with the presence of cardiovascular disease (CVD) in 362 T2DM patients included in the Diabetes and Lifestyle Cohort Twente-1 (DIALECT-1) study. Lifestyle exposures, including nutrition, were determined by validated questionnaires. CVD was defined as coronary artery diseases, strokes, and peripheral artery diseases. Median serum calprotectin levels were 1.04 mg/L [IQR: 0.73–1.46 mg/L] and were higher in women (1.11 mg/L) than men (0.96 mg/L, p = 0.007). Current smoking was a major independent determinant of circulating calprotectin, with a 51% higher calprotectin compared to never smoking (p < 0.001). Albuminuria (p = 0.011), former smoking (p = 0.023), and intake of mono- and disaccharides (p = 0.005) also contributed independently to circulating calprotectin. Each incremental increase in calprotectin level was associated with 1.36-times higher odds for CVD (95% CI 1.04–1.77, p = 0.026). In the current study, calprotectin was the only inflammatory parameter significantly associated with CVD. The strong association of circulating calprotectin with smoking, a well-known direct cause of vascular inflammation, and also with CVD, stresses the urge for further research to define its role as a biomarker in T2DM.

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Parameters of metabolism and hemostasis in patients with type 1 diabetes mellitus and microgiopathy of different severity

Diabetes Mellitus ◽

10.14341/2072-0351-5678 ◽

2010 ◽

Vol 13 (2) ◽

pp. 75-80

Author(s):

Galina Georgievna Petrik ◽

Svetlana Anatol'evna Pavlishchuk

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Pathological Process ◽

Comprehensive Analysis ◽

Platelet Volume ◽

Aggregation Activity ◽

Lipid Spectrum ◽

Altered Lipid

Aim. To identify risk factors of macro-microvascular lesions at different stages of pathological process in patients with type 1 diabetes mellitus andmicrogiopathy of different severity. Materials and methods. Comprehensive analysis of parameters of metabolism, hemogram, platelet and plasma hemostasis in 121 patients (67 menand 54 women of mean age 28.2?10.7 years) with type 1 diabetes mellitus and angiopathy of different severity. Results. All patients had hyperglycemia along with dysproteinemia and altered lipid spectrum. Mean platelet volume and aggregation activity increasedwhile activated partial thromboplastic time decreased compared with control subjects regardless of the presence and severity of microangiopathy. Conclusion. Morphological and functional characteristics of platelets and activated partial throm-boplastic time are differently related to metabolicchanges.

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Lipid profile and markers of vascular inflammation in patients with stable angina in the presence and absence of type 2 diabetes mellitus

Diabetes Mellitus ◽

10.14341/7959 ◽

2017 ◽

Vol 20 (3) ◽

pp. 194-200 ◽

Cited By ~ 1

Author(s):

Tatiana I. Petelina ◽

Natalia A. Musikhina ◽

Liudmila I. Gapom ◽

Irina V. Емеneva ◽

Elena A. Gorbatenko

Keyword(s):

Diabetes Mellitus ◽

Lipid Profile ◽

Stable Angina ◽

Inflammatory Markers ◽

Vascular Inflammation ◽

Increased Risk ◽

Presence And Absence ◽

Group 2 ◽

Group 1

Background. The mechanisms underlying the close relationship between diabetes mellitus (DM) and coronary artery disease (CAD) have not been fully understood. The pathophysiological processes of vascular inflammation that accelerate and enhance the development of atherosclerotic vascular lesions and their complications warrant further study. Aims. To compare lipid profiles and inflammatory markers in patients with CAD and stable angina in the presence and absence of type 2 DM. Materials and methods. A total of 169 patients were examined: Group 1 included 123 patients with CAD but without DM; Group 2 consisted of 46 patients with CAD and DM. The biochemical parameters were estimated before coronary angiography. Results. The lipid profile in both groups of patients revealed elevated levels of atherogenic markers (TС, LDL, VLDLP and TG). Patients in Group 2 had significantly increased levels of vascular inflammatory markers (hs-CRP, homocysteine, IL-1), TNF-, MMP-9 and endothelin-1 compared to those in Group 1. Conclusions. Our results indicate that the vascular inflammatory response was more pronounced in patients with CAD and DM and that they have an increased risk of developing adverse vascular complications.

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COVID-19 and the Heart and Vasculature

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvbaha.120.314513 ◽

2020 ◽

Vol 40 (9) ◽

pp. 2045-2053 ◽

Cited By ~ 1

Author(s):

Bernard S. Kadosh ◽

Michael S. Garshick ◽

Juan Gaztanaga ◽

Kathryn J. Moore ◽

Jonathan D. Newman ◽

...

Keyword(s):

Diabetes Mellitus ◽

Heart Disease ◽

Vascular Inflammation ◽

Severe Disease ◽

Therapeutic Interventions ◽

Cardiometabolic Disease ◽

Synergistic Activation ◽

Translational Studies ◽

Novel Therapeutic ◽

Scientific Framework

The coronavirus disease 2019 (COVID-19) pandemic presents an unprecedented challenge and opportunity for translational investigators to rapidly develop safe and effective therapeutic interventions. Greater risk of severe disease in COVID-19 patients with comorbid diabetes mellitus, obesity, and heart disease may be attributable to synergistic activation of vascular inflammation pathways associated with both COVID-19 and cardiometabolic disease. This mechanistic link provides a scientific framework for translational studies of drugs developed for treatment of cardiometabolic disease as novel therapeutic interventions to mitigate inflammation and improve outcomes in patients with COVID-19.

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Arterial hypertension and type 2 diabetes mellitus in patients with metabolic disorders: Their impact on the lipid spectrum

Arterial’naya Gipertenziya (Arterial Hypertension) ◽

10.18705/1607-419x-2003-9-2-67-70 ◽

2003 ◽

Vol 9 (2) ◽

pp. 67-70 ◽

Cited By ~ 1

Author(s):

A. I. Kuzin ◽

M. A. Cherednikova ◽

A. A. Vasilyev ◽

O. V. Kamerer

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Arterial Hypertension ◽

Metabolic Disorders ◽

The Body ◽

Cholesterol Levels ◽

Type 2 Dm ◽

Lipid Spectrum

The study was undertaken to examine the specific features of the lipid spectrum in patients with metabolic disorders in the absence or presence of arterial hypertension (AH) and type 2 diabetes mellitus (DM) The study covered 176 patients with metabolic disorders, 138 patients were diagnosed as having AH, of them 39 patients had type 2 DM Clinical and laboratory studies involved the estimation of the body mass index, waist/hip ratio, and lipid spectrum All the patients were divided into 3 groups I) 38 without AH and type 2 DM, 2) 99 with isolated AH without type 2 DM, 3) 39 with AH and type 2 DM The higher severity of abdominal obesity was associated with enhanced dyslipidemia atherogemcity The development of AH m the presence of metabolic disorders was accompanied by higher total cholesterol levels, which may be considered as aggravated endothelial dysfunction Its concurrence with type 2 DM was attended by transformation of metabolic to diabetic dyslipidemia The study has yielded a new index that characterizes dyslipidemia atherogemcity in patients with metabolic disorders

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Blood lipid spectrum in patients with non-insulin-dependent diabetes mellitus and coronary heart disease

Problems of Endocrinology ◽

10.14341/probl199844310-12 ◽

1998 ◽

Vol 44 (3) ◽

pp. 10-12 ◽

Cited By ~ 2

Author(s):

L. V. Kurashvili ◽

E. A. Semechkina ◽

T. S. Adonina ◽

G. F. Zueva ◽

I. R. Zakharova

Keyword(s):

Diabetes Mellitus ◽

Coronary Heart Disease ◽

Blood Lipid ◽

Insulin Dependent Diabetes Mellitus ◽

Dependent Diabetes Mellitus ◽

Carbohydrate And Lipid Metabolism ◽

Structural Disorders ◽

Coronary Patients ◽

Insulin Dependent ◽

Lipid Spectrum

Cholesterol and triglyceride levels in HDL are compared in diabetics and coronary patients. Carbohydrate and lipid metabolism are compared by universal manual and semiautomated methods. Measurements of individual components of the serum and of HDLP fraction in both groups showed that HDL fraction is rich in triglycerides in both diseases, that is, HDL are modified and acquire a certain role in triglyceride metabolism. Measurements of triglycerides and cholesterol in HDL fraction helps detect structural disorders in this fraction in diabetics and coronary patients; this method is more informative than measurements of total cholesterol in the blood.

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