Clogging constitutes a major operational issue for treatment wetlands. The rest period is a key feature of French Vertical Flow (VF) treatment wetlands and serves to mitigate clogging. An ex-situ drying experiment was performed to mimic the rest period and record structural changes in the porous media using X-ray Computed Tomography (CT). Samples containing the deposit and gravel layers of a first stage French VF treatment wetland were extracted and left to dry in a control environment. Based on CT scans, three phases were identified (voids, biosolids, and gravels). The impact of the rest period was assessed by means of different pore-scale variables. Ultimately, the volume of biosolids had reduced to 58% of its initial value, the deposit layer thickness dropped to 68% of its initial value, and the void/biosolid specific surface area ratio increased from a minimum value of 1.1 to a maximum of 4.2. Cracks greater than 3 mm developed at the uppermost part of the deposit layer, while, in the gravel layer, the rise in void volume corresponds to pores smaller than 2 mm in diameter. Lastly, the air-filled microporosity is estimated to have increased by 0.11 v/v.
Structural changes that occur upon photolysis of the Fe(II)a3–CO complex in the cytochrome ba3-oxidase of Thermus thermophilus: A combined X-ray crystallographic and infrared spectral study demonstrates CO binding to CuB
