Genomic structure and multiple alternative transcripts of the mouse TRAD/RAD51L3/RAD51D gene, a member of the recA/RAD51 gene family

AbstractPowdery mildew (PM) caused by Podosphaera aphanis is a major fungal disease in cultivated strawberry. Mildew Resistance Locus O (MLO) is a gene family described for having conserved seven-transmembrane domains. Induced loss-of-function in specific MLO genes can confer durable and broad resistance against PM pathogens. However, the underlying biological role of MLO genes in strawberry is still unknown. In the present study, the genomic structure of MLO genes were characterized in both diploid (Fragaria vesca) and octoploid strawberry (Fragaria ×ananassa), and the potential sources of MLO-mediated susceptibility were identified. Twenty MLO-like sequences were identified in F. vesca, with sixty-eight in F. ×ananassa. Phylogenetic analysis divides strawberry MLO genes into eight different clades, in which three FveMLO and ten FaMLO genes were grouped together with the functionally known MLO susceptibility. Out of ten FaMLO genes, FaMLO17-2 and FaMLO17-3 showed the highest similarity to the known susceptibility MLO proteins. Gene expression analysis of FaMLO genes was conducted using a multi-parental segregating population. Three expression quantitative trait loci (eQTL) were substantially associated with MLO transcript levels in mature fruits, suggesting discrete genetic control of susceptibility. These results are a critical first step in understanding allele function of MLO genes, and are necessary for further genetic studies of PM resistance in cultivated strawberry.

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Protein disulphide isomerase family in bread wheat (Triticum aestivum L.): genomic structure, synteny conservation and phylogenetic analysis

Plant Genetic Resources ◽

10.1017/s1479262111000232 ◽

2011 ◽

Vol 9 (2) ◽

pp. 342-346 ◽

Cited By ~ 1

Author(s):

E. d'Aloisio ◽

A. R. Paolacci ◽

A. P. Dhanapal ◽

O. A. Tanzarella ◽

E. Porceddu ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Gene Family ◽

Bread Wheat ◽

Plant Species ◽

Genomic Structure ◽

Triticum Aestivum L ◽

Protein Disulphide Isomerase ◽

Homoeologous Genes ◽

Genes Encoding ◽

High Level

Eight genes encoding protein disulphide isomerase (PDI)-like proteins in bread wheat were cloned and characterized and their genomic structure was compared with that of homoeologous genes isolated from other plant species. Fourteen wheat cDNA sequences of PDI-like genes were amplified and cloned; eight of them were relative to distinct PDI-like genes, whereas six corresponded to homoeologous sequences. Also, the genomic sequences of the eight non-homoeologous genes were amplified and cloned. Phylogenetic analysis, which included eight genes encoding PDI-like proteins and the gene encoding the typical PDI, assigned at least one of them to each of the eight major clades identified in the phylogenetic tree of the PDI gene family of plants. The close chromosome synteny between wheat and rice was confirmed by the location of the homoeologous genes of the PDI family in syntenic regions of the two species. Within the same phylogenetic group, a high level of conservation, in terms of sequence homology, genomic structure and domain organization, was detected between wheat and the other plant species. The high level of conservation of sequence and genomic organization within the PDI gene family, even between distant plant species, might be ascribed to the key metabolic roles of their protein products.

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Porcine kallikrein gene family: Genomic structure, mapping, and differential expression analysis

Genomics ◽

10.1016/j.ygeno.2006.11.010 ◽

2007 ◽

Vol 89 (3) ◽

pp. 429-438 ◽

Cited By ~ 8

Author(s):

S.C. Fernando ◽

F.Z. Najar ◽

X. Guo ◽

L. Zhou ◽

Y. Fu ◽

...

Keyword(s):

Gene Family ◽

Differential Expression ◽

Expression Analysis ◽

Genomic Structure ◽

Differential Expression Analysis ◽

Structure Mapping

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Genomic structure and transcriptional regulation of the penaeidin gene family from Litopenaeus vannamei

Gene ◽

10.1016/j.gene.2005.11.028 ◽

2006 ◽

Vol 371 (1) ◽

pp. 75-83 ◽

Cited By ~ 63

Author(s):

Nuala A. O'Leary ◽

Paul S. Gross

Keyword(s):

Transcriptional Regulation ◽

Gene Family ◽

Litopenaeus Vannamei ◽

Genomic Structure

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Complex 5′ Genomic Structure of the Human Prolactin Receptor: Multiple Alternative Exons 1 and Promoter Utilization

Endocrinology ◽

10.1210/endo.143.6.8949 ◽

2002 ◽

Vol 143 (6) ◽

pp. 2139-2142 ◽

Cited By ~ 21

Author(s):

Zhang-Zhi Hu ◽

Li Zhuang ◽

Jianping Meng ◽

Chon-Hwa Tsai-Morris ◽

Maria L. Dufau

Keyword(s):

Genomic Structure ◽

Prolactin Receptor ◽

Exon 2 ◽

Multiple Promoters ◽

Exon 1 ◽

Multiple Alternative ◽

Alternatively Spliced ◽

Genomic Regions ◽

Basal Transcriptional Activity ◽

Half Site

Abstract Transcription of the prolactin receptor (PRLR) is under the control of multiple promoters. Following the recent demonstration of the human non-coding exon 1, hE1N (hE1N1) and the generic exon 1 hE13, we have identified their promoters and characterized four other novel human exons 1 (hE1N2–5) that are alternatively spliced to a common non-coding exon 2 in human tissues and breast cancer cells. Genomic regions containing these exons, and 5′-flanking and intronic sequences, were determined and their order was established in chromosome 5p14-13. Promoters utilized in the transcription of previously characterized PRLR exons 1 species hE13 (hPII) and hE1N1 (hPN1) were found to employ distinct mechanisms for controlling hPRLR transcription. hPIII requires C/EBPβ and Sp1/Sp3 for basal transcriptional activity, while hPN1 activity is conferred by domains containing an Ets element and an NR half-site. The complex promoter control system that governs transcription of the hPRLR in multiple tissues is of relevance for studies on the regulation of PRLR expression in physiological and pathological states.

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