Genetic predisposition to high anxiety- and depression-like behavior coincides with diminished DNA methylation in the adult rat amygdala

Epigenetic mechanisms such as DNA methylation elicit lasting changes in gene expression and likely mediate gene–environment interactions that shape brain development, behavior, and emotional health. Myriad environmental factors influence DNA methylation, including methyl donor content in the paternal diet, could influence methylation in offspring via changes in the paternal germ line. The present study examines the effects of paternal methyl donor dietary deficiency on offspring’s emotional behaviors, including anxiety, social interaction, and depression-like behavior. We previously found that rats bred to display high levels of anxiety- and depression-like behavior exhibit diminished DNA methylation in the amygdala. We also observed that depleting dietary methyl donor content exacerbated the rats’ already high levels of anxiety- and depression-like behavior. Here we sought to determine whether paternal dietary methyl donor depletion elicits intergenerational effects on first generation (F1) offspring’s behavior (potentially triggering a similar increase in anxiety- and/or depression-like behavior). Thus, adult male rats prone to high anxiety/depression-like behavior, were fed either a methyl donor depleted (DEP) or control (CON) diet for 5 weeks prior to mating. They were paired with females and resultant F1 male offspring were subjected to a behavioral test battery in adulthood. F1-DEP offspring showed a similar behavioral profile to the F0 males, including greater depression-like behavior in the forced swim test (FST) and increased anxiety-like behavior in the open field test (OFT). Future work will interrogate molecular changes in the brains of F1 offspring that mediate these intergenerational effects of paternal methyl donor dietary content on offspring emotional behavior.

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Maternal anxiety and depression in pregnancy and DNA methylation of the NR3C1 glucocorticoid receptor gene

Epigenomics ◽

10.2217/epi-2020-0022 ◽

2020 ◽

Author(s):

Alexandra E Dereix ◽

Rachel Ledyard ◽

Allyson M Redhunt ◽

Tessa R Bloomquist ◽

Kasey JM Brennan ◽

...

Keyword(s):

Dna Methylation ◽

Depressive Symptoms ◽

Trait Anxiety ◽

Cpg Island ◽

Depression Scale ◽

Maternal Anxiety ◽

Anxiety And Depression ◽

Pregnancy Anxiety ◽

Glucocorticoid Receptor Gene ◽

Island Shore

Aim: To quantify associations of anxiety and depression during pregnancy with differential cord blood DNA methylation of the glucorticoid receptor ( NR3C1). Materials & methods: Pregnancy anxiety, trait anxiety and depressive symptoms were collected using the Pregnancy Related Anxiety Scale, State-Trait Anxiety Index and Edinburgh Postnatal Depression Scale, respectively. NR3C1 methylation was determined at four methylation sites. Results: DNA methylation of CpG 1 in the NR3C1 CpG island shore was higher in infants born to women with high pregnancy anxiety (β 2.54, 95% CI: 0.49–4.58) and trait anxiety (β 1.68, 95% CI: 0.14–3.22). No significant association was found between depressive symptoms and NR3C1 methylation. Conclusion: We found that maternal anxiety was associated with increased NR3C1 CpG island shore methylation.

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Perinatal exposure to venlafaxine leads to lower anxiety and depression-like behavior in the adult rat offspring

Behavioural Pharmacology ◽

10.1097/fbp.0000000000000393 ◽

2018 ◽

Vol 29 (5) ◽

pp. 445-452 ◽

Cited By ~ 5

Author(s):

Eszter Bogi ◽

Kristina Belovicová ◽

Eduard Ujhazy ◽

Mojmír Mach ◽

Romana Koprdova ◽

...

Keyword(s):

Anxiety And Depression ◽

Perinatal Exposure ◽

Adult Rat ◽

Rat Offspring

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Tissue- and developmental-stage-specific methylation in the two kidney promoters of the rat γ-glutamyl transpeptidase gene

Biochemical Journal ◽

10.1042/bj2870691 ◽

1992 ◽

Vol 287 (3) ◽

pp. 691-694 ◽

Cited By ~ 5

Author(s):

J H Baik ◽

S Siegrist ◽

G Giuili ◽

O Lahuna ◽

F Bulle ◽

...

Keyword(s):

Dna Methylation ◽

Developmental Stage ◽

Rat Liver ◽

Kidney Development ◽

Rat Kidney ◽

Liver Development ◽

Gamma Glutamyl Transpeptidase ◽

Site Specific ◽

Adult Rat ◽

Glutamyl Transpeptidase

We have investigated, using DNA methylation patterning, the site-specific methylation of promoters I and II of the rat gamma-glutamyl transpeptidase gene. This analysis was done in fetal, newborn and adult rat kidney, in which promoters I and II are progressively active during development, as well as in rat liver, which never expresses mRNAs from these two promoters. During kidney development, a progressive demethylation occurs in the promoter I and II region, specially at the level of the most proximal MspI site of promoter II. A progressive reorganization of the methylated sites within the 5′ end of the gene also occurs during liver development.

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Effects of varicocele on DNA methylation pattern ofH19andSnrpngene in spermatozoa and behavioural characteristics of adult rat offspring

Andrologia ◽

10.1111/and.12591 ◽

2016 ◽

Vol 49 (1) ◽

pp. e12591

Author(s):

Q. Zhang ◽

F. Zhang ◽

H-H. Gao ◽

J-M. Zhang

Keyword(s):

Dna Methylation ◽

Methylation Pattern ◽

Adult Rat ◽

Rat Offspring ◽

Behavioural Characteristics ◽

Dna Methylation Pattern

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Association between psychological risk factors and ischaemic heart disease in the open population of a large industrial centre of Eastern Siberia

Siberian medical review ◽

10.20333/25000136-2021-5-47-53 ◽

2021 ◽

Vol 5 ◽

pp. 47-53

Author(s):

R.D. Peskovets ◽

◽

S.Yu. Shtarik ◽

Keyword(s):

Risk Factors ◽

Heart Disease ◽

Ischaemic Heart Disease ◽

Group Analysis ◽

Depression Scale ◽

Anxiety And Depression ◽

Female Group ◽

High Anxiety ◽

Open Population ◽

The City

Aim of study. To study gender-based association of high anxiety and depression with ischaemic heart disease exemplified by open population of the city of Krasnoyarsk. Material and methods. A representative sample formed from unoccupied population permanently residing in the city of Krasnoyarsk aged 25-64. Psychometric testing was performed via the Hospital Anxiety and Depression Scale (HADS), the prevalence of ischaemic heart disease was determined based on conventional epidemiological criteria. Results. High values of age-adjusted prevalence of anxiety and depression in the studied sample amounted to 40.1% and 35.0% respectively with no association with the gender. Ischaemic heart disease (IHD) was registered in 10.6% of the patients (15.8% male and 8.6% female subjects, p=0.092). Statistically significant association between IHD and high anxiety (OR=2.39; 95% CI 1.19-4.8; p=0.013) and depression (OR=2.1; 95% CI 1.1-4.1; p=0.031). Gender-discrete analysis has shown analogical associations of IHD with anxiety (OR=4.41; 95% CI 1.57-12.38; р=0.004) and depression (OR=2.91; 95% CI 1.20-7.10; р=0.027) in the female group. Analysis has shown absence of interrelation between the studied risk factors and IHD in the male group of patients. Conclusion. The factor of gender defines association between high anxiety and depression scores according to the HADS in open population of the city of Krasnoyarsk.

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Depression und Angst bei Glaukom

Karger Kompass Ophthalmologie ◽

10.1159/000518800 ◽

2021 ◽

pp. 1-2

Author(s):

Klaus Rohrschneider

Keyword(s):

Logistic Regression ◽

Regression Analysis ◽

Logistic Regression Analysis ◽

Anxiety And Depression ◽

High Anxiety ◽

Confidential Interval ◽

Disc Hemorrhage ◽

Anxiety Group ◽

Thickness Loss ◽

Rnfl Thickness

Glaucoma is considered a chronic disease that requires lifelong management. Chronic diseases are known to be highly associated with psychological disturbances such as depression and anxiety. There have also been many studies on association between anxiety or depression and glaucoma. The majority of these studies explained that the glaucoma diagnosis causes anxiety or depression. However, It is also necessary to evaluate whether the psychological disturbance itself affect glaucoma. Therefore, we investigated the association of anxiety and depression with glaucoma progression, and elucidate mechanisms underlying that. We included 251 eyes with open angle glaucoma who were followed up for at least 2 years in this retrospective case-control study. The Beck Anxiety Inventory (BAI) and Beck Depressive Inventory-II (BDI-II) were used to assess anxiety and depression in glaucoma patients. Patients were classified into groups (high-anxiety group; HA-G, low-anxiety group; LA-G, high-depression group; HD-G, low-depression group; LD-G) according to their score on the BAI or BDI-II (separately). In logistic regression analysis, disc hemorrhage, peak intraocular pressure (IOP) and RNFL thickness loss rate were significantly associated with high anxiety (p = 0.017, p = 0.046, p = 0.026). RNFL thinning rate and disc hemorrhage were significant factors associated with anxiety in multivariate models (p = 0.015, p = 0.019). Multivariate linear regression analysis showed a significant positive correlation between the rate of RNFL thickness loss and BAI score (B = 0.058; 95% confidential interval = 0.020–0.097; p = 0.003), and RNFL loss and IOP fluctuation (B = 0.092; 95% confidential interval = 0.030–0.154; p = 0.004). For the depression scale, visual field mean deviation and heart rate variability were significantly associated with high depression in multivariate logistic regression analysis (p = 0.003, p = 0.006). We suggest that anxiety increase the risk of glaucoma progression and they are also associated with IOP profile and disc hemorrhage.

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