Triple mutation of Cys26, Trp35, and Cys126 in stromal ascorbate peroxidase confers H2O2 tolerance comparable to that of the cytosolic isoform

2008 ◽  
Vol 372 (4) ◽  
pp. 918-923 ◽  
Author(s):  
Sakihito Kitajima ◽  
Masahito Kitamura ◽  
Namiko Koja
Keyword(s):  
Ascorbate Peroxidase ◽  
Triple Mutation

Foliar Application of Abscisic Acid and Sulfonamide Compounds Induced Drought Tolerance in Watermelon

2015 ◽  
Vol 723 ◽  
pp. 705-710
Author(s):  
Wei Shun Cheng ◽  
Dan Li Zeng ◽  
Na Zhang ◽  
Hong Xia Zeng ◽  
Xian Feng Shi ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Ascorbate Peroxidase ◽  
Glycine Betaine ◽  
Foliar Application ◽  
Citrullus Lanatus ◽  
Recovery Phase ◽  
Daily Application

The effects of exogenous abscisic acid and two sulfonamide compounds: Sulfacetamide and Sulfasalazine were studied on tolerance of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus] under drought stress and compared with abscisic acid effects. Eight-week old plants were treated with ABA (10 and 25 mg/L), Sulfacetamide (25, 50 and 100 mg/L) and Sulfasalazine (25,50 and 100 mg/L). Solutions were sprayed daily and sampling was done at 0 h, 48 h, 96 h, 144 h and 48 h after re-watering (recovery phase or 192 h). Treated plants showed relatively greater drought tolerance. This indicates that, Sulfacetamide and Sulfasalazine may improve resistance in watermelon, like ABA, increasing levels of proline, glycine betaine and malondialdehyde and the activity of ascorbate peroxidase. Daily application of Sulfasalazine and Sulfacetamide during drought stress period was effective in increasing watermelon plants tolerance to drought as was ABA.

scholarly journals Change of Ascorbic Acid Level after Grafting of Tomato Seedlings

1999 ◽  
Vol 54 (9-10) ◽  
pp. 830-833 ◽  
Author(s):  
Akira Wadano ◽  
Mitsuharu Azeta ◽  
Shin-ichi Itotani ◽  
Ai Kanda ◽  
Toshio Iwaki ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Water Stress ◽  
Ascorbate Peroxidase ◽  
Phosphate Buffer ◽  
Acid Level ◽  
Active Oxygen ◽  
Ascorbic Acid Level ◽  
Electrochemical Detector ◽  
Tomato Seedlings ◽  
Severe Water Stress

Grafting is an easy way to produce a new seedling, which can tolerate against various stresses. During the acclimation after grafting, however, the seedlings still suffer a severe water stress. It is well known that water stress produces active oxygen to oxidize ascorbic acid. The concentration of ascorbic acid in the leaves was analyzed by HPLC equipped with an electrochemical detector. The column used was SP-120-5-ODS-BP (DAISO, JAPAN) and elution was performed with 0.1 ᴍ phosphate buffer, pH 3.0. After grafting the seedlings were acclimated under a 6-hr light/dark regimen. The content of ascorbic acid increased gradually during 2 days compared with control. The ascorbate peroxidase showed about constant activity, so the increase of ascorbic acid may be due to its requirement to cure the grafting

scholarly journals Kinetic study of the inactivation of ascorbate peroxidase by hydrogen peroxide

2000 ◽  
Vol 348 (2) ◽  
pp. 321 ◽  
Author(s):  
Alexander N.P. HINER ◽  
José Neptuno RODRÍGUEZ-LÓPEZ ◽  
Marino B. ARNAO ◽  
Emma LLOYD RAVEN ◽  
Francisco GARCÍA-CÁNOVAS ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Kinetic Study ◽  
Ascorbate Peroxidase
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