Advanced Glycation End-Products affect transcription factors regulating insulin gene expression

2010 ◽  
Vol 395 (1) ◽  
pp. 122-125 ◽  
Author(s):  
A. Puddu ◽  
D. Storace ◽  
P. Odetti ◽  
G.L. Viviani
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Advanced Glycation End Products ◽  
Insulin Gene ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Insulin Gene Expression

scholarly journals Advanced Glycation End Products Inhibit Production and Activity of Matrix Metalloproteinase-2 in Human Umbilical Vein Endothelial Cells

2007 ◽  
Vol 35 (5) ◽  
pp. 709-715 ◽  
Author(s):  
L Gao ◽  
L Kang ◽  
Q Chen ◽  
C Chen ◽  
B Xu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Conditioned Medium ◽  
Advanced Glycation End Products ◽  
Umbilical Vein ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Umbilical Vein Endothelial Cells ◽  
Expression And Activity

The aim of this study was to investigate the effects of advanced glycation end products (AGEs) on the expression and activity of matrix metalloproteinases-2 (MMP-2) in human umbilical vein endothelial cells (HUVECs). Cultured HUVECs were incubated with various concentrations of AGEs-modified albumin or unmodified albumin for different time periods. Protein and gene expression of MMP-2 and the receptor for AGEs (RAGE) were measured by Western blot and reverse transcription-polymerase chain reaction, respectively. The activity of MMP-2 in the conditioned medium was measured by gelatin zymography. The AGE-modified albumin inhibited MMP-2 but increased RAGE protein and gene expression in HUVECs in a concentration- and time-dependent manner. An inhibition of MMP-2 activity was also detected in the conditioned medium of HUVECs incubated with AGEs-modified albumin. In conclusion, AGEs inhibited the expression and activity of MMP-2 in HUVECs; this may be mediated through upregulation of RAGE.

scholarly journals Potential of an Interorgan Network Mediated by Toxic Advanced Glycation End-Products in a Rat Model

Nutrients ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Shinya Inoue ◽  
Takanobu Takata ◽  
Yusuke Nakazawa ◽  
Yuka Nakamura ◽  
Xin Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Advanced Glycation End Products ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Specific Gene ◽  
Advanced Glycation ◽  
High Fructose ◽  
Glycation End Products ◽  
End Products ◽  
Liver And Kidney

Excessive intake of glucose and fructose in beverages and foods containing high-fructose corn syrup (HFCS) plays a significant role in the progression of lifestyle-related diseases (LSRD). Glyceraldehyde-derived advanced glycation end-products (AGEs), which have been designated as toxic AGEs (TAGE), are involved in LSRD progression. Understanding of the mechanisms underlying the effects of TAGE on gene expression in the kidneys remains limited. In this study, DNA microarray analysis and quantitative real-time polymerase chain reaction (PCR) were used to investigate whether HFCS-consuming Wister rats generated increased intracellular serum TAGE levels, as well as the potential role of TAGE in liver and kidney dysfunction. HFCS consumption resulted in significant accumulation of TAGE in the serum and liver of rats, and induced changes in gene expression in the kidneys without TAGE accumulation or upregulation of receptor for AGEs (RAGE) upregulation. Changes in specific gene expression profiles in the kidney were more correlated with TAGE levels in the liver tissue than in the serum. These findings suggest a direct or indirect interaction may be present between the liver and kidneys that does not involve serum TAGE or RAGE. The involvement of internal signal transduction factors such as exosomes or cytokines without IL-1β and TNF-α is suggested to contribute to the observed changes in kidney gene expression.

Sign in / Sign up

Export Citation Format

Share Document