Self-assembly formed by a short DNA probe pair: Application for highly sensitive mRNA species detection without reverse transcription

A highly sensitive electrochemical nanosensor was developed using covalent modification of a glassy carbon electrode (GCE) by self-assembly of a novel Schiff base.

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Lab in a tube: a fast-assembled colorimetric sensor for highly sensitive detection of oligonucleotides based on a hybridization chain reaction

RSC Advances ◽

10.1039/c5ra04613k ◽

2015 ◽

Vol 5 (55) ◽

pp. 44714-44721 ◽

Cited By ~ 8

Author(s):

Siqi Zhang ◽

Kun Wang ◽

Zhenyu Li ◽

Zhongmin Feng ◽

Ting Sun

Keyword(s):

Complex Formation ◽

Dna Sequence ◽

Self Assembly ◽

The Self ◽

Hybridization Chain Reaction ◽

Chain Reaction ◽

Highly Sensitive ◽

G Quadruplex ◽

Colored Product ◽

Highly Sensitive Detection

Upon adding THBV, the self-assembly of THBV with H1 allows the rest of the DNA sequence of H1 to accelerate H1–H2 complex formation. The G-quadruplex at the end of the H1–H2 complex could catalyze TMB into a colored product.

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Characterization of a fusion cDNA (RARA/myl) transcribed from the t(15;17) translocation breakpoint in acute promyelocytic leukemia

Molecular and Cellular Biology ◽

10.1128/mcb.12.2.800-810.1992 ◽

1992 ◽

Vol 12 (2) ◽

pp. 800-810

Author(s):

K S Chang ◽

S A Stass ◽

D T Chu ◽

L L Deaven ◽

J M Trujillo ◽

...

Keyword(s):

Cdna Library ◽

Acute Promyelocytic Leukemia ◽

Blot Analysis ◽

Blot Hybridization ◽

Promyelocytic Leukemia ◽

Dna Probe ◽

Translocation Breakpoint ◽

Chromosome 15 ◽

Dot Blot ◽

Mrna Species

A nonrandom chromosomal translocation breakpoint, t(15;17)(q22;q21), is found in almost all patients with acute promyelocytic leukemia (APL). Most of these breakpoints occur within the second intron of the retinoic acid receptor-alpha (RARA) gene. We screened a cDNA library of APL and have identified and sequenced a cDNA transcribed from the t(15;17) translocation breakpoint. The 5' end of cDNA p1715 consists of 503 bp of the RARA exon II sequence. A 1.76-kb cDNA without homology to any known gene available in GenBank was found truncated downstream. This cDNA sequence was assigned to chromosome 15 by dot blot hybridization of the flow cytometry-sorted chromosomes. We designate this fusion cDNA RARA/myl, which is different from myl/RARA reported by de The et al. (H. de The, C. Chomienne, M. Lanotte, L. Degos, and A. Dejean, Nature (London) 347:558-561, 1990). This result demonstrates that the two different types of hybrid mRNA can be transcribed from this breakpoint. We screened a non-APL cDNA library and identified a 2.8-kb myl cDNA. This cDNA is able to encode a polypeptide with a molecular weight of 78,450. Alternative splicing of the myl gene which resulted in myl proteins with different C terminals was found. Southern blot analysis of the genomic DNA isolated from 17 APL patients by using the myl DNA probe demonstrated that the myl gene in 12 samples was rearranged. Northern (RNA) blot analysis of RARA gene expression in two APL RNA samples showed abnormal mRNA species of 4.2 and 3.2 kb in one patient and of 4.8 and 3.8 kb in another patient; these were in addition to the normal mRNA species of 3.7 and 2.7-kb. The myl DNA probe detected a 2.6-kb abnormal mRNA in addition to the normal mRNA species of 3.2, 4.2, and 5.5 kb. Using the polymerase chain reaction, we demonstrated that both RARA/myl and myl/RARA were coexpressed in samples from three different APL patients. From this study, we conclude that the t(15;17) translocation breakpoint results in the transcription of two different fusion transcripts which are expected to be translated into fusion proteins.

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Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.01681-06 ◽

2006 ◽

Vol 45 (2) ◽

pp. 600-603 ◽

Cited By ~ 54

Author(s):

B. Hoffmann ◽

T. Harder ◽

E. Starick ◽

K. Depner ◽

O. Werner ◽

...

Keyword(s):

Avian Influenza ◽

Real Time ◽

Reverse Transcription ◽

Influenza A ◽

H5n1 Virus ◽

Reverse Transcription Pcr ◽

Highly Sensitive

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Self-assembly of ultrathin gold nanowires and single walled carbon nanotubes as a highly sensitive substrate for surface enhanced Raman spectroscopy

New Journal of Chemistry ◽

10.1039/c6nj00774k ◽

2016 ◽

Vol 40 (9) ◽

pp. 7286-7289 ◽

Cited By ~ 1

Author(s):

Yuanchao Zhang ◽

Jingquan Liu ◽

Da Li ◽

Fuhua Yan ◽

Xin Wang ◽

...

Keyword(s):

Carbon Nanotubes ◽

Raman Spectroscopy ◽

Self Assembly ◽

Single Walled Carbon Nanotubes ◽

Surface Enhanced Raman Spectroscopy ◽

Gold Nanowires ◽

Highly Sensitive ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Walled Carbon Nanotubes

Self-assembly of ultrathin gold nanowires and single-walled carbon nanotubes as highly sensitive substrates for surface enhanced Raman spectroscopy.

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Dye encapsulated zinc-based metal-organic framework as a dual-emission sensor for highly sensitive detection of antibiotics

Dalton Transactions ◽

10.1039/d1dt03950d ◽

2021 ◽

Author(s):

Kang Wang ◽

Yuhan Duan ◽

Jiajing Chen ◽

Haiying Wang ◽

Hui-Yan Liu

Keyword(s):

Self Assembly ◽

Metal Organic Framework ◽

Sensitive Detection ◽

Dual Emission ◽

Highly Sensitive ◽

Metal Organic ◽

Highly Sensitive Detection ◽

Organic Framework

Self-assembly of two Zn-MOFs, [Zn2L(DMF)3]•H2O•5DMF (1) and [Zn2L(H2O)2]•4H2O•3DMF (2) were achieved with an amide-functionalized tetracarboxylate ligand under similar conditions. Incorporated amide groups make the tetratopic linkers exhibit different configurations, tetrahedron...

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Graphene Templated DNA Arrays and Biotin-Streptavidin Sensitive Bio-Transistors Patterned by Dynamic Self-Assembly of Polymeric Films Confined within a Roll-on-Plate Geometry

Nanomaterials ◽

10.3390/nano10081468 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1468

Author(s):

Sangheon Jeon ◽

Jihye Lee ◽

Rowoon Park ◽

Jeonghwa Jeong ◽

Min Chan Shin ◽

...

Keyword(s):

Graphene Oxide ◽

Self Assembly ◽

Patterned Surfaces ◽

Dna Arrays ◽

Simple Strategy ◽

Modified Dna ◽

Highly Sensitive ◽

Wide Range ◽

Surface Chemistries ◽

Plate Geometry

Patterning of surfaces with a simple strategy provides insights into the functional interfaces by suitable modification of the surface by novel techniques. Especially, highly ordered structural topographies and chemical features from the wide range of interfaces have been considered as important characteristics to understand the complex relationship between the surface chemistries and biological systems. Here, we report a simple fabrication method to create patterned surfaces over large areas using evaporative self-assembly that is designed to produce a sacrificial template and lithographic etch masks of polymeric stripe patterns, ranging from micrometer to nanoscale. By facilitating a roll-on-plate geometry, the periodically patterned surface structures formed by repetitive slip-stick motions were thoroughly examined to be used for the deposition of the Au nanoparticles decorated graphene oxide (i.e., AuNPs, ~21 nm) and the formation of conductive graphene channels. The fluorescently labeled thiol-modified DNA was applied on the patterned arrays of graphene oxide (GO)/AuNPs, and biotin-streptavidin sensitive devices built with graphene-based transistors (GFETs, effective mobility of ~320 cm2 V−1 s−1) were demonstrated as examples of the platform for the next-generation biosensors with the high sensing response up to ~1 nM of target analyte (i.e., streptavidin). Our strategy suggests that the stripe patterned arrays of polymer films as sacrificial templates can be a simple route to creating highly sensitive biointerfaces and highlighting the development of new chemically patterned surfaces composed of graphene-based nanomaterials.

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A novel fluorescent peptidyl probe for highly sensitive and selective ratiometric detection of Cd(ii) in aqueous and bio-samplesviametal ion-mediated self-assembly

New Journal of Chemistry ◽

10.1039/c8nj02298d ◽

2018 ◽

Vol 42 (22) ◽

pp. 18143-18151 ◽

Cited By ~ 5

Author(s):

Kwan Ho Jung ◽

Semin Oh ◽

Joohee Park ◽

Yu Jin Park ◽

See-Hyoung Park ◽

...

Keyword(s):

Self Assembly ◽

Live Cells ◽

Self Assembling ◽

Highly Sensitive ◽

Ratiometric Detection

A fluorescent peptidyl probe based on a Cd(ii)-triggered self-assembling process was proposed for ratiometric detection for Cd(ii) in urine and live cells.

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