Production and single step purification of cyclodextrin glycosyltransferase from alkalophilic Bacillus firmus by ion exchange chromatography

2008 ◽  
Vol 39 (3) ◽  
pp. 510-515 ◽  
Author(s):  
Laxman S. Savergave ◽  
Santosh S. Dhule ◽  
Vitthal V. Jogdand ◽  
Sanjay N. Nene ◽  
Ramchandra V. Gadre
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step ◽  
Bacillus Firmus ◽  
Cyclodextrin Glycosyltransferase ◽  
Single Step Purification ◽  
Alkalophilic Bacillus

scholarly journals A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense.

1994 ◽  
Vol 299 (3) ◽  
pp. 825-829 ◽  
Author(s):  
U Gerber ◽  
U Jucknischke ◽  
S Putzien ◽  
H L Fuchsbauer
Keyword(s):  
Ion Exchange ◽  
Storage Stability ◽  
Culture Fluid ◽  
Strong Acid ◽  
Weak Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step ◽  
Simple Method ◽  
High Yields

Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.

Expanded and fixed bed ion exchange chromatography for the recovery of C-phycocyanin in a single step by using lysed cells

2014 ◽  
Vol 93 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Caroline Costa Moraes ◽  
Luisa Sala ◽  
Joana da Costa Ores ◽  
Anna Rafaela Cavalcante Braga ◽  
Jorge Alberto Vieira Costa ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Fixed Bed ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step

Purification of C-Phycocyanin from Spirulina platensis by Single-Step Ion-Exchange Chromatography

2011 ◽  
Vol 73 (3-4) ◽  
pp. 291-296 ◽  
Author(s):  
Xiaoxia Liao ◽  
Bochao Zhang ◽  
Xiaoqin Wang ◽  
Huidan Yan ◽  
Xuewu Zhang
Keyword(s):  
Ion Exchange ◽  
Spirulina Platensis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step

Single-step protein purification by back flush in ion exchange chromatography

2009 ◽  
Vol 392 (2) ◽  
pp. 174-176 ◽  
Author(s):  
Ming-Kai Chern ◽  
Wei-Jyh Shiah ◽  
Jyun-Jie Chen ◽  
Tzung-You Tsai ◽  
Hsin-Yin Lin ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Protein Purification ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step

Studies on an Inhibitor of Plasminogen Activation in Human Serum

1973 ◽  
Vol 30 (02) ◽  
pp. 414-424 ◽  
Author(s):  
Ulla Hedner
Keyword(s):  
Ion Exchange ◽  
Human Serum ◽  
Antithrombin Iii ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Specific Adsorption ◽  
Partial Purification ◽  
Plasminogen Activation ◽  
Preparative Electrophoresis

SummaryA procedure is described for partial purification of an inhibitor of the activation of plasminogen by urokinase and streptokinase. The method involves specific adsorption of contammants, ion-exchange chromatography on DEAE-Sephadex, gel filtration on Sephadex G-200 and preparative electrophoresis. The inhibitor fraction contained no antiplasmin, no plasminogen, no α1-antitrypsin, no antithrombin-III and was shown not to be α2 M or inter-α-inhibitor. It contained traces of prothrombin and cerulo-plasmin. An antiserum against the inhibitor fraction capable of neutralising the inhibitor in serum was raised in rabbits.

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