Systemic acropedal influence of endophyte seed treatment on Acyrthosiphon pisum and Aphis fabae offspring development and reproductive fitness

2012 ◽  
Vol 61 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Juliet Akello ◽  
Richard Sikora
Keyword(s):  
Seed Treatment ◽  
Acyrthosiphon Pisum ◽  
Reproductive Fitness ◽  
Aphis Fabae ◽  
Offspring Development

DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

1999 ◽  
Vol 131 (2) ◽  
pp. 229-230 ◽  
Author(s):  
C.K. Chan ◽  
D.J. Petersen ◽  
T.C. Vrain
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Acyrthosiphon Pisum ◽  
Random Amplified Polymorphic Dna ◽  
Aphis Gossypii ◽  
Aphis Fabae ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Laboratory Colonies ◽  
Polymerase Chain

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

scholarly journals Diversity of aphid pests on the crops in Kanchanpur district, Nepal

2020 ◽  
Vol 31 (1) ◽  
pp. 95-106
Author(s):  
Mahadev Bista
Keyword(s):  
Acyrthosiphon Pisum ◽  
Rhopalosiphum Padi ◽  
Aphis Gossypii ◽  
Aphis Fabae ◽  
Macrosiphum Euphorbiae ◽  
Agricultural Crops ◽  
Rhopalosiphum Maidis ◽  
Aphis Craccivora Koch ◽  
Macrosiphum Rosae ◽  
Different Parts

A survey was conducted to explore the aphid pests’ diversity in Kanchanpur district, far-western, Nepal from April 2018 to March 2019. A total of 15 aphid pests were collected and identified, viz. Acyrthosiphon pisum (Harris), Aphis craccivora (Koch), Aphis gossypii (Glover), Aphis fabae (Scopoli), Aphis nerii Boyer de Fonscolombe, Brevicoryne brassicae (Linnaeus), Lipaphis erysmi (Kaltenbach), Macrosiphum euphorbiae (Thomas), Macrosiphum rosae (Linnaeus), Myzus persicae (Sulzer), Rhopalosiphum maidis (Fitch), Rhopalosiphum padi (Linnaeus), Rhopalosiphum rufiadominale (Sasaki), Uroleucon compositae (Theobald), and the guava aphid. Exploration of aphid pests showed that they caused serious damages to agricultural crops by attacking different parts of plants.

scholarly journals Diversity of Bacteria Associated with Natural Aphid Populations

2003 ◽  
Vol 69 (12) ◽  
pp. 7216-7223 ◽  
Author(s):  
S. Haynes ◽  
A. C. Darby ◽  
T. J. Daniell ◽  
G. Webster ◽  
F. J. F. van Veen ◽  
...  
Keyword(s):  
Acyrthosiphon Pisum ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Natural Populations ◽  
Geographical Location ◽  
Laboratory Culture ◽  
Aphid Species ◽  
Aphis Fabae ◽  
Rrna Gene ◽  
Gradient Gel Electrophoresis ◽  
Aphid Populations

ABSTRACT The bacterial communities of aphids were investigated by terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis analysis of 16S rRNA gene fragments generated by PCR with general eubacterial primers. By both methods, theγ -proteobacterium Buchnera was detected in laboratory cultures of six parthenogenetic lines of the pea aphid Acyrthosiphon pisum and one line of the black bean aphid Aphis fabae, and one or more of four previously described bacterial taxa were also detected in all aphid lines except one of A. pisum. These latter bacteria, collectively known as secondary symbionts or accessory bacteria, comprised three taxa of γ-proteobacteria (R-type [PASS], T-type [PABS], and U-type [PAUS]) and a rickettsia (S-type [PAR]). Complementary analysis of aphids from natural populations of four aphid species (A. pisum [n= 74], Amphorophora rubi [n= 109], Aphis sarothamni [n= 42], and Microlophium carnosum [n= 101]) from a single geographical location revealed Buchnera and up to three taxa of accessory bacteria, but no other bacterial taxa, in each aphid. The prevalence of accessory bacterial taxa varied significantly among aphid species but not with the sampling month (between June and August 2000). These results indicate that the accessory bacterial taxa are distributed across multiple aphid species, although with variable prevalence, and that laboratory culture does not generally result in a shift in the bacterial community in aphids. Both the transmission patterns of the accessory bacteria between individual aphids and their impact on aphid fitness are suggested to influence the prevalence of accessory bacterial taxa in natural aphid populations.

scholarly journals Plum Pox in North America: Identification of Aphid Vectors and a Potential Role for Fruit in Virus Spread

2004 ◽  
Vol 94 (8) ◽  
pp. 868-874 ◽  
Author(s):  
Frederick Gildow ◽  
Vern Damsteegt ◽  
Andrew Stone ◽  
William Schneider ◽  
Douglas Luster ◽  
...  
Keyword(s):  
Myzus Persicae ◽  
Acyrthosiphon Pisum ◽  
Rhopalosiphum Padi ◽  
Aphid Species ◽  
Aphis Fabae ◽  
Macrosiphum Euphorbiae ◽  
Plum Pox Virus ◽  
Long Distance ◽  
Metopolophium Dirhodum ◽  
Aphid Vectors

Thirteen aphid species were tested for their ability to transmit Pennsylvania isolates of Plum pox virus (PPV) collected in Columbia (PENN-3), Franklin (PENN-4), and York (PENN-7) Counties, PA. Four species, Aphis fabae, A. spiraecola, Brachycaudus persicae, and Myzus persicae, consistently transmitted PPV in preliminary transmission tests. Two species, Metopolophium dirhodum and Rhopalosiphum padi, were occasional inefficient vectors. Toxoptera citricida, from Florida, also was an effective vector but it does not occur in major stone-fruit-growing states. Species not transmitting PPV in parallel tests included Acyrthosiphon pisum, Aphis glycines, Aulacorthum solani, Macrosiphum euphorbiae, Rhopalosiphum maidis, and Sitobion avenae. When given a 3-day probing access period simultaneously on PPV-infected peach seedlings and healthy peach seedlings, Myzus persicae, Aphis spiraecola, A. fabae, and B. persicae transmitted PPV to 63, 31, 38, and 32% of the healthy peach seedlings, respectively. When given a similar probing period on PPV-infected peach fruit and healthy peach seedlings, the same aphid species transmitted PPV to 50, 35, 0, and 0% of seedlings, respectively. Results support the hypothesis of secondary PPV spread by indigenous aphids in Pennsylvania, and suggest that PPV-infected fruit has the potential to function as a virus source for long-distance dispersal.

EFFECTS OF THE CHEMOSTERILANT THIOTEPA ON THE SEXUPARAE AND SEXUALES OF THE PEA APHID, ACYRTHOSIPHON PISUM (HOMOPTERA: APHIDIDAE)

1983 ◽  
Vol 115 (2) ◽  
pp. 215-217
Author(s):  
M. L. Sharma ◽  
L. M. Thériault
Keyword(s):  
Myzus Persicae ◽  
Acyrthosiphon Pisum ◽  
Brevicoryne Brassicae ◽  
Pea Aphid ◽  
Aphis Fabae ◽  
Macrosiphum Euphorbiae ◽  
Partial Sterility

Several chemosterilants have been tested against insects (Borkovec 1966). As a result, a total or partial sterility and decrease in fecundity have been noticed in several insects. Amongst Aphididae, Brevicoryne brassicae L., Myzus persicae Sulzer, Aphis fabae Scop., Macrosiphum euphorbiae Thomas, and Acyrthosiphon pisum (Harris) have been tested to determine the effects of chemosterilants like tepa, thiotepa, and metepa (Bonnemaison 1966; Bhalla and Robinson 1966, 1968; Chawla et al. 1973, 1974; Sharma et al. 1980; Steffan and Stüben 1976). In all previous work, either viviparae or their nymphal instars were the object of chemosterilant treatment and up to 95% reduction in fecundity was observed. Nothing to date has been described on the effect of chemosterilants on the sexuparae or sexuales of Aphididae. To continue with the results obtained by Sharma and Thériault (1980) and Sharma et al. (1980) on the viviparae, this paper describes the effects of 0.5% thiotepa [Tris (1-aziridinyl) phosphine sulphide] on sexuparae of the pea aphid, Acyrthosiphon pisum (Harris), and their progeny.

scholarly journals CYP4 and CYP6 gene variability in genome of Aphis fabae mordvilkoi Börner & Janisch, 1922

2018 ◽  
Vol 22 ◽  
pp. 108-112
Author(s):  
N. V. Voronova ◽  
M. M. Varabyova ◽  
Yu. V. Bondarenko
Keyword(s):  
Acyrthosiphon Pisum ◽  
Single Gene ◽  
Aphis Fabae ◽  
Whole Genome ◽  
Trophic Specialization ◽  
University Of Utah ◽  
Genome Data ◽  
Gene Copies ◽  
Genomic Core ◽  
Gene Variability

Aim. To estimate the variability of genes of 4th and 6th families of CYP450, which were extracted from the whole genome data of Aphis fabae mordvilkoi collected from Philadelphus coronaries L. in Belarus. Methods. The whole genome sequencing was carried out in the University of Utah DNA Sequencing and Genomic Core Facilities (USA). CYP4 and CYP6 gene sequences were extracted from the whole genome data by sequential mapping the whole genome reads to CYP4 and CYP6 CDSs of three reference genomes (Acyrthosiphon pisum Harris, 1776, Myzus persicae (Sulžer, 1776) и Diuraphis noxia (MordvilkoexKurdjumov, 1913)). All found uniqueversion of assembling were taken as a single gene. Results. In A. fabae mordvilkoi genome we found out 31 CYP4 genes and 24 from them were copies of CYP4C1s. We also found out 19 CYP6 gene sand 8 from them were identified as CYP6A13s. Variability of nucleotide an damino acid sequences of CYP4 and CYP6 CDSs were high. Conclusions. In A. fabae mordvilkoi genome most CYP4genes were identified as CYP4C1 and most CYP6 genes were CYP6A13s. Other CYP4 and CYP6 were mostly presented as single copies of different genes.Keywords: aphids, cytochrome p450, Aphis fabae, trophic specialization, gene copies.

The influence of natural enemies on wing induction in Aphis fabae and Megoura viciae (Hemiptera: Aphididae)

2007 ◽  
Vol 98 (1) ◽  
pp. 59-62 ◽  
Author(s):  
G. Kunert ◽  
K. Schmoock-Ortlepp ◽  
U. Reissmann ◽  
S. Creutzburg ◽  
W.W. Weisser
Keyword(s):  
Natural Enemies ◽  
Acyrthosiphon Pisum ◽  
Aphis Gossypii ◽  
Aphid Species ◽  
Pea Aphid ◽  
Aphis Fabae ◽  
Chrysoperla Carnea ◽  
Cotton Aphid ◽  
Aphid Density ◽  
Megoura Viciae

AbstractPrevious studies have shown that the aphid species, Aphis fabae Scopoli and Megoura viciae Buckton, do not produce winged offspring in the presence of natural enemies, in contrast to results for the pea aphid (Acyrthosiphon pisum (Harris)) and the cotton aphid (Aphis gossypii Glover); but these studies did not involve exposing aphids directly to natural enemies. We exposed colonies of both A. fabae and M. viciae to foraging lacewing (Chrysoperla carnea (Stephens)) larvae and found that the predators did not induce winged morphs among offspring compared to unexposed controls. Colonies of A. fabae responded to an increase in aphid density with increasing winged morph production, while such response was not found for M. viciae. We suggest that different aphid species differ in their susceptibility to natural enemy attack, as well as in their sensitivity to contact.

EFFECTS OF CONTACT DURATION OF 0.5% THIOTEPA ON THE ADULT VIVIPARAE AND SUBSEQUENT GENERATIONS OF THE PEA APHID, ACYRTHOSIPHON PISUM (HOMOPTERA: APHIDIDAE)

1980 ◽  
Vol 112 (12) ◽  
pp. 1315-1316 ◽  
Author(s):  
M. L. Sharma ◽  
L. M. Thériault ◽  
A. Gareau
Keyword(s):  
Acyrthosiphon Pisum ◽  
Fourth Instar ◽  
Pea Aphid ◽  
Aphis Fabae ◽  
Reproductive Capacity ◽  
Considerable Reduction ◽  
Contact Duration ◽  
Cabbage Aphid ◽  
Delayed Effects ◽  
The Third

The compound tris (1-aziridinyl) phosphine sulphide, known by the trivial name thiotepa, has been tested against few aphids. Considerable reduction in fecundity occurred due to thiotepa treatment of the cabbage aphid, Brevicoryne brassicae (L.) (Bonnemaison 1966). In 0.3% thiotepa-treated Aphis fabae Scop. a 90% decrease in reproductive capacity was observed by Steffan and Stüben (1976). Sharma and Thériault (1980) tested 0.5% thiotepa on various nymphal instars of the pea aphid, Acyrthosiphon pisum (Harris), and observed up to 83% decrease in fecundity of those treated in first and second instars. The third and fourth instar aphids were much less influenced by thiotepa. The effects of thiotepa on the adult viviparae of the pea aphid are unknown and nothing has been recorded on the transmissibility of thiotepa-caused sterility to progeny. Further to the results of Sharma and Thériault (1980) this paper describes the effects of two contact periods with 0.5% thiotepa on the adult viviparae of A. pisum and delayed effects on subsequent generations.

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