Bioinsecticide Production from Cigarette Wastes

Tobacco, one of the most significant nonfood crops, is critical to agriculture worldwide. The tobacco processing business creates a significant amount of hazardous tobacco waste containing nicotine, and only a tiny portion of it gets recycled. Nicotine, the primary component of tobacco products such as cigarettes, is an alkaloid and can be used as an insecticide. This research aims to extract nicotine from discarded cigarette butts and utilize it as an insecticide. Extraction, emulsification, and efficiency testing on cabbage aphids are all part of the procedure. The initial extraction tests used a solvent combination of ethanol and methanol in various ratios, with a 3 : 1 ratio yielding the best results. Temperature (30–60°C), extraction length (4–6 hrs), and sodium hydroxide concentration (1–3 M) are the independent variables studied for extraction parameters, and the optimal conditions are determined using Design-Expert, response surface approach central composite design (RSM-CCD). In addition, artificial neural network (ANN) studies with MATLAB were used to accurately forecast extraction yield. The extracted product was evaluated using a gas chromatography–mass spectrometry (GC-MS) and a UV/visible spectrophotometer. The ideal crude extract yield and nicotine content were 17.75 and 3.26%, respectively, at the optimal conditions of temperature 60°C, time 4 hrs, and NaOH concentration 2.83 M with desirability of 0.832. The nicotine extracted was emulsified by combining the crude extract with a combination of palm oil and surfactants. Density, viscosity, pH, flash point, and surface tension of the emulsified concentration were measured and reported as 1.01 ± 0.01 g/ml, 585.33 ± 2.52 mPa s, 9.37 ± 0.03, 87.96 C, and 34.10 mN/m, respectively. On the cabbage aphid, the emulsified concentrated extract performed best at a ratio of 1 : 100 (emulsified concentrated to solvent).

Specialist aphids feeding causes local activation of salicylic and jasmonic acid signaling in Arabidopsis veins

Aphids, the phloem sap feeders, probe into leaf tissues and activate a complex network of plant defence responses. Phytohormonal signaling plays a major role in this network; however, the dynamics of the signals spreading is yet to be clarified. Despite the growing knowledge about transcriptomic changes upon infestation, results often differ due to sampling, varying strongly between the tissues collected at the single feeding site, individual leaves, pooled infested leaves, or whole plant rosettes. This study focuses on activation of salicylic and jasmonic acid signals in Arabidopsis leaves during infestation by cabbage aphid (Brevicoryne brassicae) in high spatio-temporal resolution. We used genetically encoded fluorescent biosensors, histochemistry and qRT-PCR to precisely map activation of distinct branches of phytohormonal signaling. We found a rapid induction of salicylic and jasmonic acid signaling markers in cells surrounding stylet puncture, co-localizing with callose deposition. For both PR1 and JAZ10 we detected activation at 24 hpi, increasing and spreading along the veins until 72 hpi and, to a lesser extent, within the epidermal pavement cells. The SA signaling wave appeared in parallel with JA-associated, and continued to increase in time. Our results first show a local activation of SA- and JA-related responses after stylet penetration of Arabidopsis leaves and bring a detailed insight into the spatio-temporal complexity of plant defence activation during specialist aphid attack.

Diversity of Arthropods and Population Dynamics of Diamondback Moth Visiting Organically Cultivated Cabbage

The biodiversity of insects visiting cabbage and population dynamics of diamondback moth was studies on Golden acre variety of cabbage. The experiment was conducted at experimental plot of Ramakrishna Mission Vivekananda Educational and Research Institute, Morabadi, Ranchi, during October to January, 2018-19. During the experiment, Bihar hairy caterpillar, Diamondback moth, Cabbage aphid, Cabbage maggot, Cabbage butterfly, Tobacco caterpillar, Ladybird beetle, Rice bug, Cricket and Spider were noticed. The population of diamondback moth was positively correlated with maximum, minimum, average temperature and maximum relative humidity and negatively correlated with minimum RH, wind speed; rainfall. The highest larval population of was recorded during 1st week of January (1st Standard Meteorological Week, SMW).

Bioefficacy of entomopathogenic mycoformulations and effect of adjuvants against nymphs of cabbage aphid, Brevicornye brassicae (Linnaeus)

Cabbage aphid, Brevicornye brassicae (Linnaeus) is one of the major insect pests of cabbage. Chemical control is mostly used to manage this pest. The present investigation was conducted to evaluate an environmentally benign approach for the pest management through different entomopathogenic mycoformulations against nymphs of B. brassicae to identify effective mycoformulation. The effect of mycoformulations supplemented with different adjuvants against nymphs of B. Brassicae was also evaluated under laboratory conditions. The bioassay studies of fungal bioformulation against nymphs of B. brassicae recorded maximum cumulative per cent mortality (53.33%) in talc formulation of L. lecanii MTCC 956 and commercial L. Lecanii formulation @12g L-1 and these were at par with each other. Maximum percent reduction of nymphal population over control 54.66 and 51.33 per cent was recorded @12 g L-1in talc formulation of L. lecanii (MTCC 956) and commercial L. Lecanii formulation ten days after treatment respectively. Laboratory studies undertaken to evaluate talc formulation of L. lecanii MTCC 956 lecanii with and without adjuvants against the nymphs of B. brassicae recorded maximum percent increase in mortality over control (7.89%) in L. lecanii formulation + tween80@1%. Results of this study signified that L. lecanii formulation, when supplemented with adjuvants enhanced the growth of L. lecanii and increased mortality of nymphs of B. brassicae and could be used as a part of the integrated pest management program.

Testing of bio-rational and synthetic pesticides to manage cabbage aphid (Brevicoryne brassicae L.) in cabbage field at Rampur, Chitwan, Nepal

Cabbage aphid (Brevicoryne brassicae L.) is an important pest of cabbage which reduces the yield and quality of the cabbage head. Farmers haven been using chemical pesticides to manage them but unfortunately these practices are toxic for human health, biodiversity and the environment. The study was conducted to test the efficacy of different bio-rational insecticides along with the chemical insecticide. ‘Green Coronet’ cabbage variety was used and the field experiment was laid out in the experimental farm of Agriculture and Forestry University (AFU), Rampur, Chitwan during the winter season of 2014. The Experiment was designed in randomized complete block design with having 7 treatments (bio-rational insecticides with chemical and control) and 3 replications. Plot size was 5.76 m2 (2.4m×2.4m) and spacing of 1 m was maintained between each blocks and plots. Field experiment showed that the highest reduction of cabbage aphid was obtained in Dimethoate (30 EC) treated plot followed by Derisom treated plot. The highest yield of cabbage head was obtained in Dimethoate treated plots (66.47 mt/ha) which was significantly at par with the Derisom (58.79 mt/ ha) treated plots. The yield for other treated plots were 47.60 mt/ha for Margosom, 43.77 mt/ha for Verticillium, 41.63 mt/ ha for Cow urine, 36.77 mt/ ha for Spinosad and control (33.45 mt/ ha) in terms of cabbage head yield. And, at the same time, natural enemies’ population was significantly lower to Dimethoate treated plots compared to bio-rational insecticides. Thus, Derisom (Derris based botanical) might be the best viable alternative in eco-friendly management of cabbage aphid considering cabbage head yield and protection of natural enemies. It was also evident from the research that Margosom (Neem based botanical) was found beneficial not only to conserve natural enemies in the cabbage field but also to minimize cabbage aphid population.