Phenazines and cyclic lipopeptides produced by Pseudomonas sp. CMR12a are involved in the biological control of Pythium myriotylum on cocoyam (Xanthosoma sagittifolium)

2019 ◽  
Vol 129 ◽  
pp. 109-114 ◽  
Author(s):  
Feyisara Eyiwumi Oni ◽  
Oluwatoyin Faith Olorunleke ◽  
Monica Höfte
Keyword(s):  
Biological Control ◽  
Pseudomonas Sp ◽  
Pythium Myriotylum ◽  
Cyclic Lipopeptides ◽  
Xanthosoma Sagittifolium

scholarly journals Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4170 ◽  
Author(s):  
Olumide Owolabi Omoboye ◽  
Niels Geudens ◽  
Matthieu Duban ◽  
Mickaël Chevalier ◽  
Christophe Flahaut ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Genome Mining ◽  
Antagonistic Activity ◽  
Pyricularia Oryzae ◽  
Pseudomonas Sp ◽  
Pythium Myriotylum ◽  
Cyclic Lipopeptides ◽  
Peptide Synthetase ◽  
Kendrick Mass Defect

Pseudomonas species are metabolically robust, with capacity to produce secondary metabolites including cyclic lipopeptides (CLPs). Herein we conducted a chemical analysis of a crude CLP extract from the cocoyam rhizosphere-derived biocontrol strain Pseudomonas sp. COW3. We performed in silico analyses on its whole genome, and conducted in vitro antagonistic assay using the strain and purified CLPs. Via LC-MS and NMR, we elucidated the structures of four novel members of the bananamide group, named bananamides D-G. Besides variability in fatty acid length, bananamides D-G differ from previously described bananamides A-C and MD-0066 by the presence of a serine and aspartic acid at position 6 and 2, respectively. In addition, bananamide G has valine instead of isoleucine at position 8. Kendrick mass defect (KMD) allowed the assignment of molecular formulae to bananamides D and E. We unraveled a non-ribosomal peptide synthetase cluster banA, banB and banC which encodes the novel bananamide derivatives. Furthermore, COW3 displayed antagonistic activity and mycophagy against Pythium myriotylum, while it mainly showed mycophagy on Pyricularia oryzae. Purified bananamides D-G inhibited the growth of P. myriotylum and P. oryzae and caused hyphal distortion. Our study shows the complementarity of chemical analyses and genome mining in the discovery and elucidation of novel CLPs. In addition, structurally diverse bananamides differ in their antimicrobial activity.

scholarly journals Antibiotic and Biosurfactant Properties of Cyclic Lipopeptides Produced by Fluorescent Pseudomonas spp. from the Sugar Beet Rhizosphere

2002 ◽  
Vol 68 (7) ◽  
pp. 3416-3423 ◽  
Author(s):  
T. H. Nielsen ◽  
D. Sørensen ◽  
C. Tobiasen ◽  
J. B. Andersen ◽  
C. Christophersen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Biological Control ◽  
Cyclic Peptide ◽  
Agricultural Soils ◽  
Pathogenic Fungi ◽  
Growth Media ◽  
Single Strain ◽  
Cyclic Lipopeptides ◽  
Peptide Moiety

ABSTRACT Cyclic lipopeptides (CLPs) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent Pseudomonas spp. To provide new and efficient strains for the biological control of root-pathogenic fungi in agricultural crops, we isolated approximately 600 fluorescent Pseudomonas spp. from two different agricultural soils by using three different growth media. CLP production was observed in a large proportion of the strains (approximately 60%) inhabiting the sandy soil, compared to a low proportion (approximately 6%) in the loamy soil. Chemical structure analysis revealed that all CLPs could be clustered into two major groups, each consisting of four subgroups. The two major groups varied primarily in the number of amino acids in the cyclic peptide moiety, while each of the subgroups could be differentiated by substitutions of specific amino acids in the peptide moiety. Production of specific CLPs could be affiliated with Pseudomonas fluorescens strain groups belonging to biotype I, V, or VI. In vitro analysis using both purified CLPs and whole-cell P. fluorescens preparations demonstrated that all CLPs exhibited strong biosurfactant properties and that some also had antibiotic properties towards root-pathogenic microfungi. The CLP-producing P. fluorescens strains provide a useful resource for selection of biological control agents, whether a single strain or a consortium of strains was used to maximize the synergistic effect of multiple antagonistic traits in the inoculum.

Parasitism of oospores of Pythium spp. by strains of Actinoplanes spp.

1993 ◽  
Vol 39 (10) ◽  
pp. 964-972 ◽  
Author(s):  
N. I. Khan ◽  
A. B. Filonow ◽  
L. L. Singleton ◽  
M. E. Payton
Keyword(s):  
Biological Control ◽  
Host Specificity ◽  
Pythium Ultimum ◽  
Pythium Myriotylum ◽  
Pythium Irregulare ◽  
Pythium Arrhenomanes ◽  
Parasite Specificity ◽  
Host Parasite ◽  
First Time

Strains of Actinoplanes spp. were evaluated for their in vitro parasitism of oospores of Pythium aphanidermatum, Pythium arrhenomanes, Pythium irregulare, Pythium myriotylum, and Pythium ultimum. Oospores of Pythium arrhenomanes, Pythium irregulare, and Pythium myriotylum were identified for the first time as hosts of Actinoplanes spp. Newly recorded parasites of oospores of Pythium spp. were Actinoplanes azureus, Actinoplanes brasiliensis, Actinoplanes caeruleus, Actinoplanes ferrugineus, Actinoplanes ianthinogenes, Actinoplanes italicus, Actinoplanes minutisporangius, Actinoplanes rectilineatus, Actinoplanes teichomyceticus, Actinoplanes utahensis, Actinoplanes violaceous, Actinoplanes yunnahenis, plus 15 strains of Actinoplanes yet to be speciated. Parasitized oospores had disorganized cytoplasms and hyphae of Actinoplanes sp. emerging from them. Infection of oospores in vitro varied from 0 to > 90%. Strains also were very active parasites of oospores in sterile soils. When added to nonsterile soils, several strains increased (p = 0.05) the level of oospore parasitism compared with nonsupplemented soils. Strains of Actinoplanes spp. exhibited a host specificity for species of Pythium in vitro and in soil. Sporulation of Actinoplanes sp. from infected oospores incubated on soil was frequent and more abundant than that observed in vitro.Key words: Pythium spp., Actinoplanes spp., actinomycetes, biological control, host–parasite specificity.

Biological control of damping-off of sugar-beet and cotton with Chaetomium globosum or a fluorescent Pseudomonas sp.

1988 ◽  
Vol 34 (5) ◽  
pp. 631-637 ◽  
Author(s):  
D. Walther ◽  
D. Gindrat
Keyword(s):  
Biological Control ◽  
Sugar Beet ◽  
Seed Treatment ◽  
Pythium Ultimum ◽  
Chaetomium Globosum ◽  
Pseudomonas Sp ◽  
Damping Off ◽  
Antifungal Metabolites ◽  
Fluorescent Pseudomonas

Seed treatment with ascospores of Chaetomium globosum reduced damping-off of sugar-beet caused by seed-borne Phoma betae and soil-borne Pythium ultimum or Rhizoctonia solani in growth chamber experiments. Seed treatment with a fluorescent Pseudomonas sp. controlled Ph. betae and P. ultimum but not R. solani. Coating cotton seeds with ascospores controlled P. ultimum and R. solani damping-off. In some experiments, biological seed treatments were equally or more effective than seed treatment with captan. However, greater variability in disease control occurred with the antagonists than with captan. Fifty percent of freshly harvested ascospores of C. globosum germinated in 8 h on water agar. When ascospores were stored under air-dried conditions for 3 days to 2.5 years, germination increased to > 90%. Under same storage conditions, survival of Pseudomonas sp. was detected after 4 months. Antagonistic activities observed in vitro were hyphal coiling of C. globosum on R. solani, and mycostasis was induced by C. globosum or Pseudomonas sp. on agar and soil. The presumed cause of mycostasis is the diffusible antifungal metabolites which may also be involved in the biological control of damping-off.

scholarly journals A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1086
Author(s):  
Matěj Pánek ◽  
Aleš Hanáček ◽  
Jana Wenzlová ◽  
Marie Maňasová ◽  
Miloslav Zouhar
Keyword(s):  
Biological Control ◽  
Plant Pathogen ◽  
Environmental Conditions ◽  
Biological Agents ◽  
Pseudomonas Sp ◽  
Phytophthora Cactorum ◽  
Biological Control Agents ◽  
Pythium Oligandrum ◽  
Clonostachys Rosea ◽  
Strawberry Cultivars

A comparison of the ability of commercially produced biological control agents—Contans, Gliorex, Hirundo, Polyversum, Prometheus, Clonoplus, Integral Pro and Xilon GR, completed with an isolate of Clonostachys rosea and of Pseudomonas sp.—to protect strawberry plants against Phytophthora cactorum was performed. The experiment was performed on strawberry cultivars Sonata, Karmen, and Wendy—cultivated in a cultivating room and greenhouse. The health of plants was affected negatively by the pathogen in all variants of biological agents used, but differences were seen in the rates of this decrease. The results revealed the ability of some tested agents to improve the growth of plants in the absence of the pathogen; the preparation Polyversum (Pythium oligandrum) was the most beneficial, in both the presence and absence of the pathogen. Contrarily, some agents alone decreased the health of plants; Integral Pro (Bacillus subtillis) and a strain of Pseudomonas sp. caused a deterioration in the health of the plants, even in the absence of a pathogen. The results of our analysis demonstrate the varied usefulness of all agents under unified environmental conditions; their effect seems to be dependent on the conditions and on the combination of the genotypes of all three participants in the interaction: plant–pathogen–antagonist.

scholarly journals Draft Genome Sequence of a Kale (Brassica oleracea L.) Root Endophyte, Pseudomonas sp. Strain C9

2017 ◽  
Vol 5 (15) ◽  
Author(s):  
Aurelie Laugraud ◽  
Sandra Young ◽  
Emily Gerard ◽  
Maureen O’Callaghan ◽  
Steven Wakelin
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Brassica Oleracea ◽  
Growth Promotion ◽  
Draft Genome ◽  
Pseudomonas Sp ◽  
Root Endophyte ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Brassica Oleracea L

ABSTRACT Pseudomonas sp. strain C9 is a plant growth–promoting bacterium isolated from the root tissue of Brassica oleracea L. grown in soil from Marlborough, New Zealand. Its draft genome of 6,350,161 bp contains genes associated with plant growth promotion and biological control.

scholarly journals Efecto de la aplicación de abonos orgánicos en la supresión de Pythium myriotylum en plantas de tiquisque (Xanthosoma sagittifolium)

2010 ◽  
Author(s):  
Silvia Artavia ◽  
Lidieth Uribe ◽  
Francisco Saborío ◽  
Luis Felipe Arauz ◽  
Leida Castro
Keyword(s):  
Pythium Myriotylum ◽  
Xanthosoma Sagittifolium ◽  
El Sistema

Los abonos orgánicos ejercen efecto supresivo sobre patógenos de planta; su capacidad supresora varía de acuerdo al tipo de abono y al sistema planta-patógeno. Se evaluó el efecto supresor de diferentes abonos orgánicos en el sistema tiquisque-Pythium myriotylum. Se determinó la madurez, estabilidad, y actividad microbiana de los diferentes abonos, así como el efecto del tipo de compostaje (compost vs vermicompost), el material de origen (estiércol vs broza de café) y el grado de madurez, sobre la supresión del patógeno. El efecto supresivo sobre el desarrollo de la enfermedad en plantas de tiquisque se estableció por medio de una escala visual de síntomas a los 3, 6 y 9 días del transplante. Los abonos presentaron relaciones C/N entre 6 y 15 y ninguno afectó la germinación o la longitud de las raíces de plántulas de pepino. Los abonos maduros clasificaron como estables y los inmaduros, con excepción del compost de broza, como inestables. La menor incidencia de pudrición de raíces por P. myriotylum se obtuvo con el uso de vermicompost a base de estiércol maduro, mientras que la menor severidad de la enfermedad ocurrió cuando el suelo se enmendó con el compost y el vermicompost maduro a base de estiércol. En términos generales los abonos obtenidos a partir de estiércol fueron más supresivos, y presentaron una mayor actividad microbiana, que los producidos a base de broza de café. Se concluye que el tipo de compostaje, el origen y el grado de madurez tienen influencia sobre la capacidad supresora.

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