scholarly journals Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4170 ◽  
Author(s):  
Olumide Owolabi Omoboye ◽  
Niels Geudens ◽  
Matthieu Duban ◽  
Mickaël Chevalier ◽  
Christophe Flahaut ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Genome Mining ◽  
Antagonistic Activity ◽  
Pyricularia Oryzae ◽  
Pseudomonas Sp ◽  
Pythium Myriotylum ◽  
Cyclic Lipopeptides ◽  
Peptide Synthetase ◽  
Kendrick Mass Defect

Pseudomonas species are metabolically robust, with capacity to produce secondary metabolites including cyclic lipopeptides (CLPs). Herein we conducted a chemical analysis of a crude CLP extract from the cocoyam rhizosphere-derived biocontrol strain Pseudomonas sp. COW3. We performed in silico analyses on its whole genome, and conducted in vitro antagonistic assay using the strain and purified CLPs. Via LC-MS and NMR, we elucidated the structures of four novel members of the bananamide group, named bananamides D-G. Besides variability in fatty acid length, bananamides D-G differ from previously described bananamides A-C and MD-0066 by the presence of a serine and aspartic acid at position 6 and 2, respectively. In addition, bananamide G has valine instead of isoleucine at position 8. Kendrick mass defect (KMD) allowed the assignment of molecular formulae to bananamides D and E. We unraveled a non-ribosomal peptide synthetase cluster banA, banB and banC which encodes the novel bananamide derivatives. Furthermore, COW3 displayed antagonistic activity and mycophagy against Pythium myriotylum, while it mainly showed mycophagy on Pyricularia oryzae. Purified bananamides D-G inhibited the growth of P. myriotylum and P. oryzae and caused hyphal distortion. Our study shows the complementarity of chemical analyses and genome mining in the discovery and elucidation of novel CLPs. In addition, structurally diverse bananamides differ in their antimicrobial activity.

scholarly journals Antimicrobial activity of extracellular metabolites from antagonistic bacteria isolated from potato (Solanum phureja) crops

2014 ◽  
Vol 40 (3) ◽  
pp. 212-220 ◽  
Author(s):  
Sinar David Granada García ◽  
Antoni Rueda Lorza ◽  
Carlos Alberto Peláez
Keyword(s):  
Antimicrobial Activity ◽  
Cyclic Peptide ◽  
Biological Activities ◽  
Pathogenic Fungi ◽  
Antagonistic Activity ◽  
Solanum Phureja ◽  
Dual Culture ◽  
Crude Extracts ◽  
Extracellular Metabolites

Microorganisms for biological control are capable of producing active compounds that inhibit the development of phytopathogens, constituting a promising tool toob tain active principles that could replace synthetic pesticides. This study evaluatedtheability of severalpotentialbiocontrol microorganismsto produce active extracellular metabolites. In vitro antagonistic capability of 50 bacterial isolates from rhizospheric soils of "criolla" potato (Solanum phureja) was tested through dual culture in this plant with different plant pathogenic fungi and bacteria. Isolates that showed significantly higher antagonistic activity were fermented in liquid media and crude extracts from the supernatants had their biological activities assessed by optical density techniques. Inhibitory effecton tested pathogens was observed for concentrations between 0.5% and 1% of crude extracts. There was a correlation between the antimicrobial activity of extracts and the use of nutrient-rich media in bacteria fermentation. Using a bioguided method, a peptidic compound, active against Fusarium oxysporum, was obtained from the 7ANT04 strain (Pyrobaculum sp.). Analysis by nuclear magnetic resonance and liquid chromatography coupled to mass detector evidenced an 11-amino acid compound. Bioinformatic software using raw mass data confirmed the presence of a cyclic peptide conformed by 11 mostly non-standard amino acids.

scholarly journals Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 442 ◽  
Author(s):  
Massimo Iorizzo ◽  
Bruno Testa ◽  
Silvia Jane Lombardi ◽  
Sonia Ganassi ◽  
Mario Ianiro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biochemical Properties ◽  
Antagonistic Activity ◽  
Paenibacillus Larvae ◽  
Bacterial Disease ◽  
Osmotic Tolerance ◽  
Potential Benefits ◽  
Honeybee Health ◽  
Exopolysaccharides Production

Paenibacillus larvae is the causative agent of American foulbrood (AFB), a severe bacterial disease that affects larvae of honeybees. The present study evaluated, in vitro, antimicrobial activity of sixty-one Lactiplantibacillus plantarum strains, against P. larvae ATCC 9545. Five strains (P8, P25, P86, P95 and P100) that showed the greatest antagonism against P. larvae ATCC 9545 were selected for further physiological and biochemical characterizations. In particular, the hydrophobicity, auto-aggregation, exopolysaccharides production, osmotic tolerance, enzymatic activity and carbohydrate assimilation patterns were evaluated. The five L. plantarum selected strains showed suitable physical and biochemical properties for their use as probiotics in the honeybee diet. The selection and availability of new selected bacteria with good functional characteristics and with antagonistic activity against P. larvae opens up interesting perspectives for new biocontrol strategies of diseases such as AFB.

scholarly journals Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

2010 ◽  
Vol 75 (6) ◽  
pp. 739-747 ◽  
Author(s):  
Dinesh Bisht ◽  
Rajendra Padalia ◽  
Lalit Singh ◽  
Veena Pande ◽  
Priyanka Lal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antagonistic Activity ◽  
Significant Activity ◽  
Variable Degree ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

scholarly journals Antagonistic activity “in vitro” of some saprophytic fungi occurring on the phylloplane of rice, wheat and maize

2019 ◽  
Vol 3 (3) ◽  
Author(s):  
A. Mangiarotti ◽  
G. Del Frate ◽  
A. M. Picco ◽  
G. Caretta
Keyword(s):  
Botrytis Cinerea ◽  
Alternaria Alternata ◽  
Penicillium Chrysogenum ◽  
Fusarium Moniliforme ◽  
Antagonistic Activity ◽  
Pyricularia Oryzae ◽  
Gibberella Zeae ◽  
Aspergillus Clavatus ◽  
Stachybotrys Atra

Fue investigada la actividad antagónica in vitro de un total de 51 cepas pertenecientes a 16 géneros fúngicos detectados con mayor frecuencia en el filoplano del centeno, arroz y maíz.El antagonismo fue observado con el método de los cultivos duales en medios con agar, entre estos hongos y los fitopatógenos: Drechslera oryzae, Pyricularia oryzae y Helminthosporium maydis. Los más antagónicos son: Penicillium chrysogenum, P. thomii y Stachybotrys atra; ellos antagonizaron en particular con Botrytis cinerea, Fusarium moniliforme y Alternaria alternata. El antagonismo más exitoso contra los tres patógenos mencionados fue con P. chrysogenum y P. charlesii, quienes fueron notoriamente activos. Las cepas de Chaetomium globosum, Gibberella zeae, Fusarium moniliforme, Trichothecium roseum y B. cinerea presentan una actividad antagónica contra H. maydis; Cladosporium cladosporioides contra P. oryzae y D. oryzae; Aspergillus clavatus frente a D. oryzae. Los resultados son discutidos en relación al control biológico de estos patógenos de cereales

Uji Antagonis Pseudomonas sp. Asal Endofit Perakaran Padi Terhadap Penyakit Blas (Pyricularia oryzae) Secara In Vitro

Agrologia ◽  
2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Andree Saylendra ◽  
Tubagus Bahtiar Rusbana ◽  
Linda Herdiani
Keyword(s):  
Pyricularia Oryzae ◽  
In Vitro Testing ◽  
Antagonistic Bacteria ◽  
Pseudomonas Sp ◽  
Test Results ◽  
Clear Zone ◽  
Pseudomonas Spp ◽  
Randomized Design ◽  
Completely Randomized Design

The purpose of this research was to determine the antagonistic bacteria Pseudomonas spp. of paddy roots endophytic against disease blast (Pycularia oryzae) in vitro. This research was conducted at the Laboratory of Agroecology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa on April to June 2015. This research implemented experimental one factor that arranged in Completely Randomized Design with three replications. The treatment consisted of 9 isolates of Pseudomonas sp. which was screened from endophytic roots of paddy plants.  The test  results  showed  that  antagonistic in  vitro  testing could inhibit the growth P. oryzae. Inhibition of 9 isolates to the growth Pseudomonas spp. had the significant clear zone. Isolate Ps 17 (58.89%), Ps 22 (54.44%), Ps 37 (52.06%) and Ps 39 (61.42%) have the largest inhibition. The results showed that the bacteria Pseudomonas sp. on this research is potential on controlling disease blast in paddy plant.

scholarly journals Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere

2020 ◽  
Vol 8 (7) ◽  
pp. 1079
Author(s):  
Feyisara E. Oni ◽  
Niels Geudens ◽  
Amayana Adiobo ◽  
Olumide O. Omoboye ◽  
Elsie A. Enow ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activities ◽  
Gene Clusters ◽  
Evolutionary Divergence ◽  
Biosynthetic Gene Clusters ◽  
Genomic Locus ◽  
White Line ◽  
Cyclic Lipopeptides ◽  
Peptide Synthetase ◽  
Complete Lysis

Pseudomonas cyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced by Pseudomonas strains isolated from the cocoyam rhizosphere in Cameroon and Nigeria. LC-MS and NMR analyses showed that the Pseudomonas sp. COR52 and A2W4.9 produce pseudodesmin and viscosinamide, respectively. These CLPs belong to the Viscosin group characterized by a nonapeptidic moiety with a 7-membered macrocycle. Similar to other Viscosin-group CLPs, the initiatory non-ribosomal peptide synthetase (NRPS) gene of the viscosinamide BGC is situated remotely from the other two NRPS genes. In contrast, the pseudodesmin genes are all clustered in a single genomic locus. Nano- to micromolar levels of pseudodesmin and viscosinamide led to the hyphal distortion and/or disintegration of Rhizoctonia solani AG2-2 and Pythium myriotylum CMR1, whereas similar levels of White Line-Inducing Principle (WLIP), another member of the Viscosin group, resulted in complete lysis of both soil-borne phytopathogens. In addition to the identification of the biosynthetic genes of these two CLPs and the demonstration of their interaction with soil-borne pathogens, this study provides further insights regarding evolutionary divergence within the Viscosin group.

scholarly journals Production of DAPG and HCN by Pseudomonas sp. LBUM300 Contributes to the Biological Control of Bacterial Canker of Tomato

2012 ◽  
Vol 102 (10) ◽  
pp. 967-973 ◽  
Author(s):  
Carine Lanteigne ◽  
Vijay J. Gadkar ◽  
Thérèse Wallon ◽  
Amy Novinscak ◽  
Martin Filion
Keyword(s):  
Biological Control ◽  
Quantitative Polymerase Chain Reaction ◽  
Antagonistic Activity ◽  
Economic Losses ◽  
Bacterial Canker ◽  
Pseudomonas Sp ◽  
In Planta ◽  
Tomato Production ◽  
Bacterial Canker Of Tomato

Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis is known to cause significant economic losses to tomato production worldwide. Biological control has been proposed as an alternative to current chemical containment methods, which are often inefficient and may leave adverse effects on the environment. However, only little headway has so far been made in developing biocontrol strategies against C. michiganensis subsp. michiganensis. To address this knowledge gap, we investigated the antagonistic capacity of PCA, produced by Pseudomonas sp. LBUM223, and DAPG and HCN, both produced by Pseudomonas sp. LBUM300, on C. michiganensis subsp. michiganensis under in vitro and in planta conditions. Nonsynthesizing isogenic mutants of the producer strains were also developed to further dissect the role of each individual metabolite on C. michiganensis subsp. michiganensis biological control. Novel specific quantitative polymerase chain reaction TaqMan assays allowed quantification of C. michiganensis subsp. michiganensis in tomato plants and rhizospheric soil. Pseudomonas spp. LBUM223 and LBUM300 significantly repressed C. michiganensis subsp. michiganensis growth in vitro, while their respective nonproducing mutants showed less or no significant antagonistic activity. In planta, only Pseudomonas sp. LBUM300 was capable of significantly reducing disease development and C. michiganensis subsp. michiganensis rhizospheric population, suggesting that the production of both DAPG and HCN was involved. In summary, simultaneous DAPG/HCN production by Pseudomonas sp. LBUM300 shows great potential for controlling bacterial canker of tomato.

scholarly journals Antiplasmodial activity and genome mining study of marine-derived Streptomyces sp. GMY01

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Ema Damayanti ◽  
Jaka Widada ◽  
Achmad Dinoto ◽  
Mustofa
Keyword(s):  
Polyketide Synthase ◽  
Genome Mining ◽  
Gene Clusters ◽  
Antiplasmodial Activity ◽  
Active Compounds ◽  
Streptomyces Sp ◽  
Ic50 Value ◽  
Peptide Synthetase ◽  
Genes Encoding

High resistance to chloroquine in most malaria-endemic area in the world leads to the need for new antimalaria drugs. Marine bacterium Streptomyces  is the source for potential new antimalarial molecules. This research aimed to investigate antiplasmodial activity of marine-derived of Streptomyces sp. GMY01 and to identify potential active compounds using genome mining study. In vitro antiplasmodial activity assays using flow cytometry method showed that the ethyl acetate extract of this bacterium had high antiplasmodial activity (IC50 value of 1.183 µg/mL) on Plasmodium falciparum FCR3. Genome mining analysis of whole-genome sequences using antiSMASH 6.0 beta version revealed that Streptomyces sp. GMY01 had 28 biosynthetic gene clusters (BGCs), including the genes encoding polyketide synthase, non-ribosomal peptide synthetase, terpene, lanthipeptide, bacteriocin, butyrolactone, ectoin, siderophore, and others. The known BGCs were predicted to be involved in the production of known compounds from gene clusters ranged from 5 to 100% similarity. Ongoing purification and elucidation of the structures will allow identification of the active compounds produced by marine-derived Streptomyces sp. GMY01.

scholarly journals Isolation and Characterisation of Probiotics for Antagonising Cariogenic Bacterium Streptococcus mutans and Preventing Biofilm Formation

2016 ◽  
Author(s):  
Pei-Pei Lin ◽  
You-Miin Hsieh ◽  
Cheng-Chih Tsai
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Antagonistic Activity ◽  
Lactobacillus Pentosus ◽  
Chewing Gums ◽  
Dependent Cell ◽  
Cariogenic Bacterium

Consumption of refined foods and beverages high in sugar make the teeth susceptible to the formation of biofilm, and lead to dental caries and diseases of the oral cavity such as periodontitis and periodontal disease. The aim of the present study was to determine the ability of selected probiotics to inhibit growth and biofilm formation by the cariogenic bacterium Streptococcus mutans in vitro. We screened strains of latic acid bacteria (LAB) (n=120) from the Bioresources Collection and Research Center (BCRC), saliva of healthy adults and infant stool. The antimicrobial activity of LAB in vitro was evaluated by agar spot culture and co-culture of the S. mutans strains. We determined the effect of heating and dilution factors (2- and 4-fold) on the antagonistic activity of LAB. Antagonistic substances in the spent culture suspensions (SCS) of LAB were precipitated by extraction with ammonium sulphate and chloroform to characterise the protein and lipophilic fractions. Results of co-culturing show that the SCS of the three LAB strains (Lactobacillus pentosus 13-1, 13-4 and Lactobacillus crispatus BCRC 14618) subjected to heat treatment showed significantly high antimicrobial activity. We found that substances produced by L. pentosus 13-4 which have the potential to exhibit antimicrobial properties might be lipophilic proteins. Additionally, we infer that the mechanism of reducing biofilm formation by Lactobacillus strains is associated with sucrose-dependent cell–cell adhesion and the gtfC level of glucosyltransferases (Gtfs) in the biofilm. Native LAB strains screened in our study may be used in chewing gums and other processed foods for preventing tooth decay.

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