Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies

Superficial bladder cancer is often treated by removing the cancerous portion of the bladder wall combined with immuno-chemotherapy; in more extreme cases, it is often necessary to remove the entire bladder wall. This diagnosis brings an obvious need for bladder tissue replacement designs with a high degree of efficacy. Since bladder cells are accustomed to interacting with extracellular matrix proteins having dimensions on the nanometer scale, this study aimed to design the next generation of tissue-engineered bladder replacement constructs with nanometer (less than 100 nm) surface features. For this purpose, porous and biodegradable PLGA and PU scaffolds were treated with various concentrations of NaOH or HNO3, respectively, for various periods of time to create nanometer surface roughness. Resulting surface properties were characterized using SEM (to visualize scaffold properties) and BET. Cell experiments conducted on these polymeric scaffolds provided evidence of enhanced bladder smooth muscle cell attachment, growth, and elastin/collagen production (critical extracellular matrix proteins in the bladder tissue regeneration process) as surface feature dimensions were reduced into the nanometer regime. In vivo augmentation surgeries with nano-structured PLGA and PU patches will provide further information regarding total bladder capacity, anastomotic integrity, burst pressure, epithelialization, muscular ingrowth, and neovascularization. In vitro and in vivo proof of material usefulness and technique would provide urologists with a readily accessible graft for bladder tissue replacement applications.

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Biomimetic titanium implant coated with extracellular matrix enhances and accelerates osteogenesis

Nanomedicine ◽

10.2217/nnm-2020-0047 ◽

2020 ◽

Vol 15 (18) ◽

pp. 1779-1793

Author(s):

Yu Wu ◽

Haikuo Tang ◽

Lin Liu ◽

Qianting He ◽

Luodan Zhao ◽

...

Keyword(s):

Bone Marrow ◽

Extracellular Matrix ◽

Stromal Cells ◽

Titanium Surface ◽

Titanium Implant ◽

Titanium Implants ◽

In Vivo Studies ◽

Calcium Deposition

Aim: To evaluate the biological function of titanium implants coated with cell-derived mineralized extracellular matrix, which mimics a bony microenvironment. Materials & methods: A biomimetic titanium implant was fabricated primarily by modifying the titanium surface with TiO2 nanotubes or sand-blasted, acid-etched topography, then was coated with mineralized extracellular matrix constructed by culturing bone marrow mesenchymal stromal cells. The osteogenic ability of biomimetic titanium surface in vitro and in vivo were evaluated. Results: In vitro and in vivo studies revealed that the biomimetic titanium implant enhanced and accelerated osteogenesis of bone marrow stromal cells by increasing cell proliferation and calcium deposition. Conclusion: By combining surface topography modification with biological coating, the results provided a valuable method to produce biomimetic titanium implants with excellent osteogenic ability.

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In vitro and in vivo studies on the metabolism of 1,3-diaminobenzene: Comparison of metabolites formed by the perfused rat liver, primary rat hepatocyte cultures, hepatic rat microsomes and the whole rat

Toxicology in Vitro ◽

10.1016/0887-2333(89)90001-5 ◽

1989 ◽

Vol 3 (3) ◽

pp. 167-174 ◽

Cited By ~ 11

Author(s):

H.C. Bisgaard ◽

H.R. Lam

Keyword(s):

Rat Liver ◽

In Vivo Studies ◽

Perfused Rat Liver ◽

Rat Hepatocyte ◽

Hepatocyte Cultures

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Nanofiber Systems as Herbal Bioactive Compounds Carriers: Current Applications in Healthcare

Pharmaceutics ◽

10.3390/pharmaceutics14010191 ◽

2022 ◽

Vol 14 (1) ◽

pp. 191

Author(s):

Kathya Huesca-Urióstegui ◽

Elsy J. García-Valderrama ◽

Janet A. Gutierrez-Uribe ◽

Marilena Antunes-Ricardo ◽

Daniel Guajardo-Flores

Keyword(s):

Extracellular Matrix ◽

Bioactive Compounds ◽

Herbal Medicines ◽

Bioactive Compound ◽

High Specific Surface Area ◽

In Vivo Studies ◽

Natural Polymers ◽

Healthcare Applications

Nanofibers have emerged as a potential novel platform due to their physicochemical properties for healthcare applications. Nanofibers’ advantages rely on their high specific surface-area-to-volume and highly porous mesh. Their peculiar assembly allows cell accommodation, nutrient infiltration, gas exchange, waste excretion, high drug release rate, and stable structure. This review provided comprehensive information on the design and development of natural-based polymer nanofibers with the incorporation of herbal medicines for the treatment of common diseases and their in vivo studies. Natural and synthetic polymers have been widely used for the fabrication of nanofibers capable of mimicking extracellular matrix structure. Among them, natural polymers are preferred because of their biocompatibility, biodegradability, and similarity with extracellular matrix proteins. Herbal bioactive compounds from natural extracts have raised special interest due to their prominent beneficial properties in healthcare. Nanofiber properties allow these systems to serve as bioactive compound carriers to generate functional matrices with antimicrobial, anti-inflammatory, antioxidant, antiseptic, anti-viral, and other properties which have been studied in vitro and in vivo, mostly to prove their wound healing capacity and anti-inflammation properties.

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Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

Pharmaceutics ◽

10.3390/pharmaceutics13091469 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1469

Author(s):

Julie R. Youssef ◽

Nabila A. Boraie ◽

Heba F. Ibrahim ◽

Fatma A. Ismail ◽

Riham M. El-Moslemany

Keyword(s):

Wound Healing ◽

Skin Regeneration ◽

In Vivo Studies ◽

Dermal Substitute ◽

Full Thickness ◽

In Vitro Characterization ◽

Polymeric Scaffolds ◽

Chitosan Composite

Skin restoration following full-thickness injury poses significant clinical challenges including inflammation and scarring. Medicated scaffolds formulated from natural bioactive polymers present an attractive platform for promoting wound healing. Glibenclamide was formulated in collagen/chitosan composite scaffolds to fulfill this aim. Glibenclamide was forged into nanocrystals with optimized colloidal properties (particle size of 352.2 nm, and polydispersity index of 0.29) using Kolliphor as a stabilizer to allow loading into the hydrophilic polymeric matrix. Scaffolds were prepared by the freeze drying method using different total polymer contents (3–6%) and collagen/chitosan ratios (0.25–2). A total polymer content of 3% at a collagen/chitosan ratio of 2:1 (SCGL3-2) was selected based on the results of in vitro characterization including the swelling index (1095.21), porosity (94.08%), mechanical strength, rate of degradation and in vitro drug release. SCGL3-2 was shown to be hemocompatible based on the results of protein binding, blood clotting and percentage hemolysis assays. In vitro cell culture studies on HSF cells demonstrated the biocompatibility of nanocrystals and SCGL3-2. In vivo studies on a rat model of a full-thickness wound presented rapid closure with enhanced histological and immunohistochemical parameters, revealing the success of the scaffold in reducing inflammation and promoting wound healing without scar formation. Hence, SCGL3-2 could be considered a potential dermal substitute for skin regeneration.

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Traversing the basement membrane in vivo: A diversity of strategies

The Journal of Cell Biology ◽

10.1083/jcb.201311112 ◽

2014 ◽

Vol 204 (3) ◽

pp. 291-302 ◽

Cited By ~ 105

Author(s):

Laura C. Kelley ◽

Lauren L. Lohmer ◽

Elliott J. Hagedorn ◽

David R. Sherwood

Keyword(s):

Extracellular Matrix ◽

Basement Membrane ◽

Metastatic Disease ◽

Tissue Level ◽

In Vitro Studies ◽

In Vivo Studies ◽

Leukocyte Trafficking ◽

Diverse Range

The basement membrane is a dense, highly cross-linked, sheet-like extracellular matrix that underlies all epithelia and endothelia in multicellular animals. During development, leukocyte trafficking, and metastatic disease, cells cross the basement membrane to disperse and enter new tissues. Based largely on in vitro studies, cells have been thought to use proteases to dissolve and traverse this formidable obstacle. Surprisingly, recent in vivo studies have uncovered a remarkably diverse range of cellular- and tissue-level strategies beyond proteolysis that cells use to navigate through the basement membrane. These fascinating and unexpected mechanisms have increased our understanding of how cells cross this matrix barrier in physiological and disease settings.

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Stachydrine ameliorates the progression of intervertebral disc degeneration via the PI3K/Akt/NF-κB signaling pathway: in vitro and in vivo studies

Food & Function ◽

10.1039/d0fo02323j ◽

2020 ◽

Vol 11 (12) ◽

pp. 10864-10875

Author(s):

Zhenxuan Shao ◽

Jiajie Lu ◽

Chenxi Zhang ◽

Guoling Zeng ◽

Boda Chen ◽

...

Keyword(s):

Extracellular Matrix ◽

Intervertebral Disc ◽

Signaling Pathway ◽

Disc Degeneration ◽

Intervertebral Disc Degeneration ◽

Inflammatory Responses ◽

In Vivo Studies ◽

Matrix Degradation

Stachydrine ameliorates inflammatory responses and extracellular matrix degradation, via the PI3K/Akt/NF-κB signalling pathway in the progression of intervertebral disc degeneration.

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